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Structure paper

TitleA structural basis for streptomycin-induced misreading of the genetic code.
Journal, issue, pagesNat Commun, Vol. 4, Page 1355-1355, Year 2013
Publish dateFeb 16, 2012 (structure data deposition date)
AuthorsDemirci, H. / Murphy, F. / Murphy, E. / Gregory, S.T. / Dahlberg, A.E. / Jogl, G.
External linksNat Commun / PubMed:23322043
MethodsX-ray diffraction
Resolution3.249 - 3.969 Å
Structure data

PDB-4dr1:
Crystal structure of the apo 30S ribosomal subunit from Thermus thermophilus (HB8)
Method: X-RAY DIFFRACTION / Resolution: 3.6 Å

PDB-4dr2:
Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with multiple copies of paromomycin molecules bound
Method: X-RAY DIFFRACTION / Resolution: 3.249 Å

PDB-4dr3:
Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with streptomycin bound
Method: X-RAY DIFFRACTION / Resolution: 3.348 Å

PDB-4dr4:
Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with codon, cognate transfer RNA anticodon stem-loop and multiple copies of paromomycin molecules bound
Method: X-RAY DIFFRACTION / Resolution: 3.969 Å

PDB-4dr5:
Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with codon, crystallographically disordered cognate transfer RNA anticodon stem-loop and streptomycin bound
Method: X-RAY DIFFRACTION / Resolution: 3.45 Å

PDB-4dr6:
Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with codon, near-cognate transfer RNA anticodon stem-loop mismatched at the first codon position and streptomycin bound
Method: X-RAY DIFFRACTION / Resolution: 3.3 Å

PDB-4dr7:
Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with codon, crystallographically disordered near-cognate transfer RNA anticodon stem-loop mismatched at the second codon position, and streptomycin bound
Method: X-RAY DIFFRACTION / Resolution: 3.75 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-PAR:
PAROMOMYCIN / Antimicrobial, medication*YM

ChemComp-SRY:
STREPTOMYCIN / medication, antibiotic*YM

Source
  • thermus thermophilus (bacteria)
KeywordsRIBOSOME / decoding / Streptomycin / RNA structure / antibiotic resistance / RIBOSOME/ANTIBIOTIC / RIBOSOME-ANTIBIOTIC complex

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