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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 4cz7 | ||||||
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タイトル | Truncated tetramerization domain of zebrafish p53 (crystal form III) | ||||||
![]() | CELLULAR TUMOR ANTIGEN P53 | ||||||
![]() | CELL CYCLE / TUMOR SUPPRESSOR / TRANSCRIPTION FACTOR PROTEIN EVOLUTION / DANIO RERIO | ||||||
機能・相同性 | ![]() regulation of oogenesis / Regulation of TP53 Expression / Regulation of TP53 Activity through Acetylation / Regulation of TP53 Activity through Association with Co-factors / Regulation of TP53 Activity through Methylation / RUNX3 regulates CDKN1A transcription / Regulation of TP53 Activity through Phosphorylation / PKR-mediated signaling / Autodegradation of the E3 ubiquitin ligase COP1 / G2/M Checkpoints ...regulation of oogenesis / Regulation of TP53 Expression / Regulation of TP53 Activity through Acetylation / Regulation of TP53 Activity through Association with Co-factors / Regulation of TP53 Activity through Methylation / RUNX3 regulates CDKN1A transcription / Regulation of TP53 Activity through Phosphorylation / PKR-mediated signaling / Autodegradation of the E3 ubiquitin ligase COP1 / G2/M Checkpoints / Ovarian tumor domain proteases / epithelium development / Ub-specific processing proteases / Oxidative Stress Induced Senescence / Oncogene Induced Senescence / Regulation of TP53 Degradation / Stabilization of p53 / The role of GTSE1 in G2/M progression after G2 checkpoint / intestinal epithelial structure maintenance / programmed cell death involved in cell development / hematopoietic stem cell homeostasis / epidermis morphogenesis / apoptotic process involved in blood vessel morphogenesis / negative regulation of cell division / retrotransposon silencing / regulation of double-strand break repair / apoptotic process involved in development / response to methylmercury / definitive hemopoiesis / regulation of intrinsic apoptotic signaling pathway by p53 class mediator / DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / hematopoietic stem cell proliferation / erythrocyte maturation / negative regulation of cell cycle / response to X-ray / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / anatomical structure morphogenesis / erythrocyte development / positive regulation of cell cycle / response to UV / intrinsic apoptotic signaling pathway / negative regulation of angiogenesis / transforming growth factor beta receptor signaling pathway / DNA damage checkpoint signaling / promoter-specific chromatin binding / protein tetramerization / regulation of protein stability / autophagy / cellular senescence / positive regulation of neuron apoptotic process / regulation of cell population proliferation / cellular response to hypoxia / spermatogenesis / sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific / cell cycle / positive regulation of apoptotic process / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / apoptotic process / DNA damage response / chromatin / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / mitochondrion / identical protein binding / nucleus / metal ion binding / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Joerger, A.C. | ||||||
![]() | ![]() タイトル: Tracing the Evolution of the P53 Tetramerization Domain 著者: Joerger, A.C. / Wilcken, R. / Andreeva, A. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 93.5 KB | 表示 | ![]() |
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PDB形式 | ![]() | 75.3 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 480.6 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 480.9 KB | 表示 | |
XML形式データ | ![]() | 10.3 KB | 表示 | |
CIF形式データ | ![]() | 14.2 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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2 | ![]()
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単位格子 |
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要素
#1: タンパク質・ペプチド | 分子量: 3799.270 Da / 分子数: 6 / 断片: TRUNCATED TETRAMERIZATION DOMAIN, RESIDUES 302-331 / 由来タイプ: 組換発現 由来: (組換発現) ![]() ![]() 発現宿主: ![]() ![]() #2: 化合物 | #3: 化合物 | ChemComp-GOL / | #4: 水 | ChemComp-HOH / | 配列の詳細 | ADDITIONAL | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2 Å3/Da / 溶媒含有率: 38 % / 解説: NONE |
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結晶化 | 温度: 293 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 5 詳細: SITTING DROP VAPOR DIFFUSION AT 20 DEGREE C; PROTEIN SOLUTION: 17 MG/ML IN 20 MM TRIS PH 7.5, 50 MM NACL, 5 MM DTT; CRYSTALLIZATION BUFFER: 1.8 M SODIUM/POTASSIUM PHOSPHATE, PH 5.0. |
-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.8266 Å / 相対比: 1 |
反射 | 解像度: 1.1→27.7 Å / Num. obs: 68831 / % possible obs: 94.8 % / Observed criterion σ(I): 2 / 冗長度: 3.5 % / Rmerge(I) obs: 0.06 / Net I/σ(I): 12.1 |
反射 シェル | 解像度: 1.1→1.16 Å / 冗長度: 3.3 % / Rmerge(I) obs: 0.17 / Mean I/σ(I) obs: 6.5 / % possible all: 87.4 |
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解析
ソフトウェア | 名称: REFMAC / バージョン: 5.8.0069 / 分類: 精密化 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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精密化 | 構造決定の手法: ![]()
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溶媒の処理 | イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 12.823 Å2
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精密化ステップ | サイクル: LAST / 解像度: 1.1→27.7 Å
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拘束条件 |
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