- PDB-4d1m: Tetramerization domain of zebrafish p53 (crystal form II) -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 4d1m
Title
Tetramerization domain of zebrafish p53 (crystal form II)
Components
CELLULAR TUMOR ANTIGEN P53
Keywords
TRANSCRIPTION / P53 FAMILY / TUMOR SUPPRESSOR / TRANSCRIPTION FACTOR / TETRAMER / PROTEIN EVOLUTION
Function / homology
Function and homology information
epithelium development / anatomical structure morphogenesis / protein tetramerization / DNA-binding transcription factor activity, RNA polymerase II-specific / cell cycle / RNA polymerase II cis-regulatory region sequence-specific DNA binding / apoptotic process / identical protein binding / nucleus / metal ion binding / cytoplasm Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 59 / Source method: isolated from a natural source / Formula: H2O
Sequence details
ADDITIONAL GGS SEQUENCE AT THE N TERMINUS AS A RESULT OF CLONING STRATEGY
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.6 Å3/Da / Density % sol: 53 % / Description: NONE
Crystal grow
Temperature: 293 K / Method: vapor diffusion, sitting drop Details: SITTING DROP VAPOR DIFFUSION AT 20 DEGREE C; PROTEIN SOLUTION: 12 MG/ML IN 20 MM TRIS PH 7.5, 50 MM NACL, 5 MM DTT; CRYSTALLIZATION BUFFER: 160 MM ZINC ACETATE, 80 MM SODIUM CACODYLATE, PH 6. ...Details: SITTING DROP VAPOR DIFFUSION AT 20 DEGREE C; PROTEIN SOLUTION: 12 MG/ML IN 20 MM TRIS PH 7.5, 50 MM NACL, 5 MM DTT; CRYSTALLIZATION BUFFER: 160 MM ZINC ACETATE, 80 MM SODIUM CACODYLATE, PH 6.5, 12% (W/V) POLYETHYLENE GLYCOL 8,000 AND 19% (W/V) GLYCEROL