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Yorodumi- PDB-4cyk: Structural basis for binding of Pan3 to Pan2 and its function in ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4cyk | ||||||
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Title | Structural basis for binding of Pan3 to Pan2 and its function in mRNA recruitment and deadenylation | ||||||
Components | PAB-DEPENDENT POLY(A)-SPECIFIC RIBONUCLEASE SUBUNIT PAN3 | ||||||
Keywords | TRANSCRIPTION / PAN3P / PAN2P / POLYA / RNA / DEADENYLATION | ||||||
Function / homology | Function and homology information PAN complex / Deadenylation of mRNA / nuclear-transcribed mRNA poly(A) tail shortening / poly(A) binding / postreplication repair / P-body / mRNA processing / protein kinase activity / DNA repair / RNA binding ...PAN complex / Deadenylation of mRNA / nuclear-transcribed mRNA poly(A) tail shortening / poly(A) binding / postreplication repair / P-body / mRNA processing / protein kinase activity / DNA repair / RNA binding / ATP binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | SACCHAROMYCES CEREVISIAE (brewer's yeast) | ||||||
Method | SOLUTION NMR / CNS 1.2 | ||||||
Authors | Wolf, J. / Valkov, E. / Allen, M.D. / Meineke, B. / Gordiyenko, Y. / McLaughlin, S.H. / Olsen, T.M. / Robinson, C.V. / Bycroft, M. / Stewart, M. / Passmore, L.A. | ||||||
Citation | Journal: Embo J. / Year: 2014 Title: Structural Basis for Pan3 Binding to Pan2 and its Function in Mrna Recruitment and Deadenylation. Authors: Wolf, J. / Valkov, E. / Allen, M.D. / Meineke, B. / Gordiyenko, Y. / Mclaughlin, S.H. / Olsen, T.M. / Robinson, C.V. / Bycroft, M. / Stewart, M. / Passmore, L.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4cyk.cif.gz | 255.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4cyk.ent.gz | 211.7 KB | Display | PDB format |
PDBx/mmJSON format | 4cyk.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4cyk_validation.pdf.gz | 257.7 KB | Display | wwPDB validaton report |
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Full document | 4cyk_full_validation.pdf.gz | 363.6 KB | Display | |
Data in XML | 4cyk_validation.xml.gz | 17.8 KB | Display | |
Data in CIF | 4cyk_validation.cif.gz | 28 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cy/4cyk ftp://data.pdbj.org/pub/pdb/validation_reports/cy/4cyk | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 4741.405 Da / Num. of mol.: 1 / Fragment: RESIDUES 1-41 Source method: isolated from a genetically manipulated source Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast) Plasmid: PHLTV / Production host: ESCHERICHIA COLI B41 (bacteria) / References: UniProt: P36102 |
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#2: Chemical | ChemComp-ZN / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR details | Text: THE STRUCTURE WAS DETERMINED USING HOMONUCLEAR AND TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N-LABELED PROTEIN |
-Sample preparation
Details | Contents: 90% WATER / 10% D2O |
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Sample conditions | Ionic strength: 100 mM / Label: conditions_1 / pH: 7.0 / Pressure: 1 atm / Temperature: 298.0 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: CNS 1.2 / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. | ||||||||||||
NMR representative | Selection criteria: fewest violations | ||||||||||||
NMR ensemble | Conformer selection criteria: NO VIOLATIONS / Conformers calculated total number: 20 / Conformers submitted total number: 20 |