+Open data
-Basic information
Entry | Database: PDB / ID: 2l93 | ||||||
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Title | Solution structure of the C-terminal domain of Salmonella H-NS | ||||||
Components | DNA-binding protein H-NS | ||||||
Keywords | DNA BINDING PROTEIN / H-NS / AT hook | ||||||
Function / homology | Function and homology information bent DNA binding / nucleoid / DNA-binding transcription repressor activity / negative regulation of gene expression, epigenetic / minor groove of adenine-thymine-rich DNA binding / protein-DNA complex / structural constituent of chromatin / protein dimerization activity / transcription cis-regulatory region binding / negative regulation of DNA-templated transcription ...bent DNA binding / nucleoid / DNA-binding transcription repressor activity / negative regulation of gene expression, epigenetic / minor groove of adenine-thymine-rich DNA binding / protein-DNA complex / structural constituent of chromatin / protein dimerization activity / transcription cis-regulatory region binding / negative regulation of DNA-templated transcription / DNA binding / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | Salmonella typhimurium (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Li, Y. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2011 Title: Structural basis for recognition of AT-rich DNA by unrelated xenogeneic silencing proteins Authors: Gordon, B.R.G. / Li, Y. / Cote, A. / Weirauch, M.T. / Ding, P. / Hughes, T.R. / Navarre, W.W. / Xia, B. / Liu, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2l93.cif.gz | 307.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2l93.ent.gz | 255.9 KB | Display | PDB format |
PDBx/mmJSON format | 2l93.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2l93_validation.pdf.gz | 401.8 KB | Display | wwPDB validaton report |
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Full document | 2l93_full_validation.pdf.gz | 476.7 KB | Display | |
Data in XML | 2l93_validation.xml.gz | 15.4 KB | Display | |
Data in CIF | 2l93_validation.cif.gz | 26.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l9/2l93 ftp://data.pdbj.org/pub/pdb/validation_reports/l9/2l93 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 6393.120 Da / Num. of mol.: 1 / Fragment: C-terminal domain, residues 91-137 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Salmonella typhimurium (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: P0A1S2 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 2D 1H-15N HSQC |
-Sample preparation
Details | Contents: 50mM [U-100% 13C; U-100% 15N] sodium phosphate; 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 50 mM / Component: sodium phosphate-1 / Isotopic labeling: [U-100% 13C; U-100% 15N] |
Sample conditions | Ionic strength: 0.05 / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 / Representative conformer: 1 |