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- PDB-4cwm: High-glycosylation crystal structure of the bifunctional endonucl... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4cwm | |||||||||
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Title | High-glycosylation crystal structure of the bifunctional endonuclease (AtBFN2) from Arabidopsis thaliana | |||||||||
![]() | ENDONUCLEASE 2 | |||||||||
![]() | HYDROLASE / SSDNA BINDING | |||||||||
Function / homology | ![]() T/G mismatch-specific endonuclease activity / Aspergillus nuclease S1 / double-stranded DNA endonuclease activity / single-stranded DNA endodeoxyribonuclease activity / DNA catabolic process / RNA endonuclease activity / endonuclease activity / nucleic acid binding / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Yu, T.-F. / Maestre-Reyna, M. / Ko, C.-Y. / Ko, T.-P. / Sun, Y.-J. / Lin, T.-Y. / Shaw, J.-F. / Wang, A.H.-J. | |||||||||
![]() | ![]() Title: Structural Insights of the Ssdna Binding Site in the Multifunctional Endonuclease Atbfn2 from Arabidopsis Thaliana. Authors: Yu, T. / Maestre-Reyna, M. / Ko, C. / Ko, T. / Sun, Y. / Lin, T. / Shaw, J. / Wang, A.H. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 134.1 KB | Display | ![]() |
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PDB format | ![]() | 103.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.2 MB | Display | ![]() |
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Full document | ![]() | 2.3 MB | Display | |
Data in XML | ![]() | 27.6 KB | Display | |
Data in CIF | ![]() | 40.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4cxoC ![]() 4cxpC ![]() 4cxvC ![]() 3w52S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 30606.156 Da / Num. of mol.: 2 / Fragment: RESIDUES 28-290 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Sugars , 3 types, 6 molecules
#2: Polysaccharide | Source method: isolated from a genetically manipulated source #3: Polysaccharide | Source method: isolated from a genetically manipulated source #4: Polysaccharide | Source method: isolated from a genetically manipulated source |
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-Non-polymers , 2 types, 463 molecules ![](data/chem/img/ZN.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/HOH.gif)
#5: Chemical | ChemComp-ZN / #6: Water | ChemComp-HOH / | |
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-Details
Nonpolymer details | ALPHA-D-MANNOSE (MAN): PART OF N-GLYCANS ZINC ION (ZN2): PART OF THE TRINUCLEAR ACTIVE SITE N- ...ALPHA-D-MANNOSE (MAN): PART OF N-GLYCANS ZINC ION (ZN2): PART OF THE TRINUCLEAR |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.19 Å3/Da / Density % sol: 43.81 % / Description: NONE |
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Crystal grow | pH: 8.5 Details: 0.1 M TRIS PH 8.5 0.2 M SODIUM ACETATE 30% W/V PEG4000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM / Detector: CCD / Date: Oct 10, 2012 / Details: MIRRORS |
Radiation | Monochromator: FIXED-EXIT DOUBLE CRYSTAL MONOCHROMATOR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.09→23.28 Å / Num. obs: 28226 / % possible obs: 97.3 % / Observed criterion σ(I): -3 / Redundancy: 3.9 % / Rmerge(I) obs: 0.01 / Net I/σ(I): 14.7 |
Reflection shell | Resolution: 2.09→2.15 Å / Redundancy: 3.9 % / Rmerge(I) obs: 0.45 / Mean I/σ(I) obs: 3.7 / % possible all: 88 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3W52 Resolution: 2.09→23.28 Å / Cor.coef. Fo:Fc: 0.943 / Cor.coef. Fo:Fc free: 0.897 / SU B: 6.187 / SU ML: 0.167 / Cross valid method: THROUGHOUT / ESU R: 0.311 / ESU R Free: 0.238 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 27.392 Å2
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Refinement step | Cycle: LAST / Resolution: 2.09→23.28 Å
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Refine LS restraints |
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