PDB-4csf: Structural insights into Toscana virus RNA encapsidation

ID or keywords:

Entry

Database: PDB / ID: 4csf

Title

Structural insights into Toscana virus RNA encapsidation

Components

(NUCLEOPROTEIN) x 3
RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

Keywords

VIRAL PROTEIN/RNA /

VIRAL PROTEIN-RNA COMPLEX /

VIRAL PROTEIN /

NUCLEOCAPSID /

NUCLEOPROTEIN /

HEXAMERIC /

INFECTIOUS

Function / homology

Function and homology information

helical viral capsid / host cell endoplasmic reticulum-Golgi intermediate compartment / host cell Golgi apparatus / viral nucleocapsid /

ribonucleoprotein complex / host cell nucleus /

Biological species

TOSCANA VIRUS
SYNTHETIC CONSTRUCT (others)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.598 Å

Authors

Olal, D. / Daumke, O.

Citation

Journal: Nucleic Acids Res. / Year: 2014
Title: Structural Insights Into RNA Encapsidation and Helical Assembly of the Toscana Virus Nucleoprotein.
Authors: Olal, D. / Dick, A. / Woods, V.L. / Liu, T. / Li, S. / Devignot, S. / Weber, F. / Saphire, E.O. / Daumke, O.

History

Deposition	Mar 7, 2014	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Apr 9, 2014	Provider: repository / Type: Initial release
Revision 1.1	Jun 4, 2014	Group: Database references
Revision 1.2	Oct 16, 2019	Group: Data collection / Experimental preparation / Other Category: exptl_crystal_grow / pdbx_database_status / reflns_shell Item: _exptl_crystal_grow.method / _pdbx_database_status.status_code_sf / _reflns_shell.Rmerge_I_obs

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	4csf.cif.gz	2.2 MB	Display	PDBx/mmCIF format
PDB format	pdb4csf.ent.gz	2 MB	Display	PDB format
PDBx/mmJSON format	4csf.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/cs/4csf ftp://data.pdbj.org/pub/pdb/validation_reports/cs/4csf	HTTPS FTP

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Related structure data

Related structure data	4csgC C: citing same article (ref.)
Similar structure data	4csg-2 4csg-1 5fva-1 6477 5fva-3 4j4r-1 4j4x-d 5fva-2 Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

Related structure data

4csgC

C: citing same article (ref.)

Similar structure data

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Links

PDB pages	PDBj / wwPDB / NCBI

Deposited unit

A: NUCLEOPROTEIN

B: NUCLEOPROTEIN

C: NUCLEOPROTEIN

D: NUCLEOPROTEIN

E: NUCLEOPROTEIN

F: NUCLEOPROTEIN

G: NUCLEOPROTEIN

H: NUCLEOPROTEIN

I: NUCLEOPROTEIN

J: NUCLEOPROTEIN

K: NUCLEOPROTEIN

L: NUCLEOPROTEIN

M: NUCLEOPROTEIN

N: NUCLEOPROTEIN

O: NUCLEOPROTEIN

P: NUCLEOPROTEIN

Q: NUCLEOPROTEIN

R: NUCLEOPROTEIN

S: NUCLEOPROTEIN

T: NUCLEOPROTEIN

U: NUCLEOPROTEIN

V: NUCLEOPROTEIN

W: NUCLEOPROTEIN

X: NUCLEOPROTEIN

a: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

c: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

e: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

g: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

i: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

k: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

m: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

o: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

q: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

s: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

u: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

w: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

699 kDa, 36 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

S: NUCLEOPROTEIN

T: NUCLEOPROTEIN

U: NUCLEOPROTEIN

V: NUCLEOPROTEIN

W: NUCLEOPROTEIN

X: NUCLEOPROTEIN

s: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

u: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

w: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

defined by author&software
175 kDa, 9 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

M: NUCLEOPROTEIN

N: NUCLEOPROTEIN

O: NUCLEOPROTEIN

P: NUCLEOPROTEIN

Q: NUCLEOPROTEIN

R: NUCLEOPROTEIN

m: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

o: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

q: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

defined by author&software
175 kDa, 9 polymers
Search similar-shape structures of this assembly by Omokage search (details)

3

G: NUCLEOPROTEIN

H: NUCLEOPROTEIN

I: NUCLEOPROTEIN

J: NUCLEOPROTEIN

K: NUCLEOPROTEIN

L: NUCLEOPROTEIN

g: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

i: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

k: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

defined by author&software
175 kDa, 9 polymers
Search similar-shape structures of this assembly by Omokage search (details)

4

A: NUCLEOPROTEIN

B: NUCLEOPROTEIN

C: NUCLEOPROTEIN

D: NUCLEOPROTEIN

E: NUCLEOPROTEIN

F: NUCLEOPROTEIN

a: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

c: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

e: RNA (5'-R(*UP*GP*UP*GP*UP*UP*UP*CP*UP)-3')

defined by author&software
175 kDa, 9 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	98.777, 127.837, 170.467
Angle α, β, γ (deg.)	82.10, 79.75, 74.49
Int Tables number	1
Space group name H-M	P1

Noncrystallographic symmetry (NCS)

NCS domain:
NCS domain segments:
NCS ensembles :

ID
1
2
3
4
5

#1: Protein	... NUCLEOPROTEIN / / NUCLEOCAPSID PROTEIN / PROTEIN N / RIBONUCLEOPROTEIN Mass: 27740.109 Da / Num. of mol.: 21 Source method: isolated from a genetically manipulated source Source: (gene. exp.) TOSCANA VIRUS / Description: SYNTHETIC / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): ROSETTA / References: UniProt: P21701
#2: Protein	NUCLEOPROTEIN / / NUCLEOCAPSID PROTEIN / PROTEIN N / RIBONUCLEOPROTEIN Mass: 27712.096 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) TOSCANA VIRUS / Description: SYNTHETIC / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): ROSETTA / References: UniProt: P21701
#3: Protein	NUCLEOPROTEIN / / NUCLEOCAPSID PROTEIN / PROTEIN N / RIBONUCLEOPROTEIN Mass: 27741.096 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) TOSCANA VIRUS / Description: SYNTHETIC / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): ROSETTA / References: UniProt: P21701
#4: RNA chain	RNA (5'-R(UPGPUPGPUPUPUPCPUP)-3') Mass: 2787.631 Da / Num. of mol.: 12 / Source method: obtained synthetically / Details: UGUGUUUCU / Source*: (synth.) SYNTHETIC CONSTRUCT (others)
#5: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 1475 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.9 Å³/Da / Density % sol: 58 % / Description: NONE
Crystal grow	Method: vapor diffusion, hanging drop / pH: 5 / Details: 11% PEG 3350, 0.1 M SODIUM MALONATE, PH 4.5-5.2

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Data collection

Diffraction	Mean temperature: 100 K
Diffraction source	Source: SYNCHROTRON / Site: BESSY / Beamline: 14.2 / Wavelength: 0.918
Detector	Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Jun 22, 2012 / Details: MIRROR
Radiation	Monochromator: DOUBLE SI 111 CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.918 Å / Relative weight: 1
Reflection	Resolution: 2.6→34.4 Å / Num. obs: 229780 / % possible obs: 94.7 % / Observed criterion σ(I): -3 / Redundancy: 2.2 % / B_iso Wilson estimate: 38.06 Å² / R_merge(I) obs: 0.09 / Net I/σ(I): 11.2
Reflection shell	Resolution: 2.59→2.61 Å / Redundancy: 2.2 % / R_merge(I) obs: 0.555 / Mean I/σ(I) obs: 2.1 / % possible all: 79.7

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Processing

Software

Name	Version	Classification
PHENIX	(PHENIX.REFINE)	refinement
XDS		data reduction
XDS		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 2.598→34.467 Å / SU ML: 0.32 / σ(F): 1.99 / Phase error: 25.66 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.2396	11464	5 %
R_work	0.2031	-	-
obs	0.205	229734	94.74 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 46.8 Å²

Refinement step

Cycle: LAST / Resolution: 2.598→34.467 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	45771	2196	0	1475	49442

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.005	48970
X-RAY DIFFRACTION	f_angle_d	0.847	66569
X-RAY DIFFRACTION	f_dihedral_angle_d	14.539	18649
X-RAY DIFFRACTION	f_chiral_restr	0.066	7737
X-RAY DIFFRACTION	f_plane_restr	0.003	8146

Refine LS restraints NCS

Ens-ID	Dom-ID	Auth asym-ID	Number	Refine-ID	Type	Rms dev position (Å)
1	1	A	1875	X-RAY DIFFRACTION	POSITIONAL
1	2	C	1875	X-RAY DIFFRACTION	POSITIONAL	0.034
1	3	E	1886	X-RAY DIFFRACTION	POSITIONAL	0.033
1	4	G	1886	X-RAY DIFFRACTION	POSITIONAL	0.019
2	1	B	1895	X-RAY DIFFRACTION	POSITIONAL
2	2	D	1895	X-RAY DIFFRACTION	POSITIONAL	0.035
2	3	F	1895	X-RAY DIFFRACTION	POSITIONAL	0.028
2	4	H	1890	X-RAY DIFFRACTION	POSITIONAL	0.034
3	1	I	1894	X-RAY DIFFRACTION	POSITIONAL
3	2	K	1894	X-RAY DIFFRACTION	POSITIONAL	0.025
3	3	M	1894	X-RAY DIFFRACTION	POSITIONAL	0.021
3	4	O	1894	X-RAY DIFFRACTION	POSITIONAL	0.022
4	1	J	1795	X-RAY DIFFRACTION	POSITIONAL
4	2	L	1795	X-RAY DIFFRACTION	POSITIONAL	0.029
4	3	N	1795	X-RAY DIFFRACTION	POSITIONAL	0.025
4	4	P	1795	X-RAY DIFFRACTION	POSITIONAL	0.041
4	5	R	1795	X-RAY DIFFRACTION	POSITIONAL	0.028
4	6	T	1795	X-RAY DIFFRACTION	POSITIONAL	0.041
4	7	V	1795	X-RAY DIFFRACTION	POSITIONAL	0.027
4	8	X	1784	X-RAY DIFFRACTION	POSITIONAL	0.023
5	1	Q	1888	X-RAY DIFFRACTION	POSITIONAL
5	2	S	1888	X-RAY DIFFRACTION	POSITIONAL	0.025
5	3	U	1884	X-RAY DIFFRACTION	POSITIONAL	0.028
5	4	W	1888	X-RAY DIFFRACTION	POSITIONAL	0.037

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.5983-2.6278	0.3538	218	0.2934	4570	X-RAY DIFFRACTION	59
2.6278-2.6588	0.3185	285	0.2772	5626	X-RAY DIFFRACTION	73
2.6588-2.6912	0.3225	311	0.2788	6100	X-RAY DIFFRACTION	80
2.6912-2.7252	0.325	364	0.2709	7116	X-RAY DIFFRACTION	92
2.7252-2.7611	0.3073	398	0.2649	7441	X-RAY DIFFRACTION	98
2.7611-2.7989	0.2945	386	0.2633	7516	X-RAY DIFFRACTION	98
2.7989-2.8388	0.3284	429	0.2615	7537	X-RAY DIFFRACTION	98
2.8388-2.8812	0.2908	406	0.2665	7414	X-RAY DIFFRACTION	98
2.8812-2.9262	0.3113	386	0.276	7552	X-RAY DIFFRACTION	98
2.9262-2.9741	0.3422	420	0.2669	7505	X-RAY DIFFRACTION	98
2.9741-3.0254	0.301	389	0.2583	7504	X-RAY DIFFRACTION	98
3.0254-3.0804	0.2808	403	0.257	7466	X-RAY DIFFRACTION	98
3.0804-3.1396	0.3142	402	0.2575	7476	X-RAY DIFFRACTION	98
3.1396-3.2036	0.2884	370	0.2573	7628	X-RAY DIFFRACTION	98
3.2036-3.2732	0.2935	408	0.2529	7443	X-RAY DIFFRACTION	98
3.2732-3.3493	0.2837	415	0.2379	7488	X-RAY DIFFRACTION	98
3.3493-3.433	0.2664	370	0.234	7546	X-RAY DIFFRACTION	98
3.433-3.5257	0.2552	381	0.2252	7494	X-RAY DIFFRACTION	98
3.5257-3.6294	0.2874	375	0.2217	7530	X-RAY DIFFRACTION	98
3.6294-3.7464	0.2325	413	0.2015	7467	X-RAY DIFFRACTION	98
3.7464-3.8801	0.2211	403	0.1944	7524	X-RAY DIFFRACTION	98
3.8801-4.0352	0.2075	395	0.1781	7452	X-RAY DIFFRACTION	98
4.0352-4.2186	0.1923	395	0.1623	7509	X-RAY DIFFRACTION	98
4.2186-4.4406	0.1811	365	0.1465	7521	X-RAY DIFFRACTION	98
4.4406-4.7181	0.1934	401	0.1551	7548	X-RAY DIFFRACTION	98
4.7181-5.0814	0.2043	389	0.1645	7450	X-RAY DIFFRACTION	98
5.0814-5.5908	0.207	417	0.1718	7519	X-RAY DIFFRACTION	98
5.5908-6.3953	0.2017	394	0.1692	7523	X-RAY DIFFRACTION	98
6.3953-8.0405	0.1732	399	0.1493	7466	X-RAY DIFFRACTION	97
8.0405-34.4696	0.1529	377	0.139	7339	X-RAY DIFFRACTION	95

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.9665	0.4738	0.0244	0.4419	0.0883	1.6203	-0.0226	0.221	0.46	-0.3665	-0.1411	0.2883	-0.5233	-0.2774	0.0614	0.9481	0.1137	-0.0362	0.6143	0.2313	0.6445	-3.0623	118.7447	3.1927
2	0.3135	-0.0042	-0.2037	0.9973	0.1383	1.1674	0.0275	0.152	0.1155	0.3443	0.204	0.1982	-0.5608	-0.2838	-0.1719	0.4274	0.0781	0.1488	0.3336	0.0159	0.3874	5.3795	104.9702	44.0323
3	2.1066	0.498	-0.0762	1.7235	0.2599	2.0721	-0.1128	-0.0361	0.1861	0.4124	0.0868	0.4725	-0.0761	-0.1521	0.0142	0.3889	0.0653	0.1506	0.3044	0.0814	0.404	2.2654	102.8569	44.5715
4	0.6379	-1.0457	0.1954	2.0883	-0.3282	1.0255	0.0745	0.1862	0.0289	0.643	0.0049	-0.1364	-0.3598	0.3351	-0.0259	0.3935	0.011	0.0322	0.3508	0.0312	0.2224	19.7862	97.2464	42.0951
5	1.5389	0.0961	-0.0381	1.1432	-0.0101	1.006	0.1406	0.2223	-0.0304	-0.0327	0.0142	0.0186	0.1248	-0.0137	-0.0343	0.233	0.0114	0.0168	0.2803	-0.0355	0.2415	20.4921	93.9559	30.0265
6	1.6532	0.7959	-0.1258	1.7703	-0.0227	1.7231	-0.1094	0.561	0.0823	-0.2756	0.3589	0.1137	-0.0667	-0.1142	-0.2433	0.3158	0.0283	0.0597	0.3946	0.0425	0.2806	13.8077	97.5735	26.5392
7	2.9932	1.0818	0.1052	2.9462	0.8549	2.3302	-0.0932	0.1363	0.0391	0.2324	0.0518	-0.0856	0.2397	0.3665	0.0821	0.3559	-0.0287	0.0655	0.4165	-0.0838	0.3634	27.8805	95.0364	25.2796
8	0.7347	0.6057	-0.4533	1.0545	-0.2877	0.4458	0.4238	-0.1626	0.6901	0.2529	-0.0445	0.4434	-0.5317	0.1668	-0.3183	0.449	-0.0212	0.0709	0.3017	0.0121	0.5872	8.8956	96.3086	54.2825
9	2.0764	-0.7153	-0.5756	1.2613	0.5059	0.6831	-0.0297	-0.1106	-0.071	0.1019	0.055	-0.0839	0.0281	0.109	-0.0018	0.2723	0.0099	-0.0269	0.2291	-0.0263	0.3282	10.905	69.053	60.6071
10	2.9099	0.2603	0.2254	3.7306	0.5549	3.1923	-0.0576	-0.5426	-0.0945	0.7241	0.1205	0.0924	-0.0347	0.0417	-0.0593	0.2822	0.032	0.0335	0.3754	-0.0514	0.2585	2.6811	69.0868	72.1367
11	1.8511	-0.1166	-0.4001	1.8215	-0.151	0.9654	-0.0062	0.0555	0.169	-0.073	0.0768	0.1168	0.139	-0.0904	-0.0039	0.22	-0.0057	-0.0661	0.164	-0.0069	0.2556	-2.4613	41.7369	37.2709
12	1.6102	-0.7544	0.276	1.7358	0.1076	1.2852	0.0036	0.1755	0.2615	-0.0581	-0.0686	-0.1049	-0.1129	0.1843	0.0308	0.2798	-0.0004	-0.0158	0.2315	0.0076	0.4858	12.7675	51.8609	37.6153
13	2.9087	0.8467	1.8023	0.6627	-0.7221	2.7888	-0.3208	0.4818	-0.2041	-0.0572	0.4025	0.2248	-0.069	0.0224	-0.1045	0.5038	0.021	-0.054	0.4142	-0.0312	0.4169	-9.4196	34.6753	27.6514
14	3.2855	0.4552	-0.2526	1.9528	0.0413	1.2808	-0.0968	0.1317	-0.2334	0.0223	0.0836	0.0403	0.317	-0.2977	0.0378	0.4724	-0.1842	-0.0693	0.5219	0.053	0.3811	-8.8332	40.2692	0.1734
15	1.5566	0.2166	0.0611	0.8157	0.6421	0.5415	0.0061	0.044	-0.3765	-0.1675	-0.2186	0.2949	0.2297	-0.3262	0.2199	0.5117	-0.1522	-0.1135	0.7032	0.0871	0.8168	-19.9957	50.5511	-10.0666
16	1.4338	0.0441	0.0882	1.8711	0.2959	1.3616	-0.1621	-0.1513	0.2376	-0.4962	0.152	0.4923	-0.5556	-0.1761	0.0981	0.5409	0.0126	-0.159	0.6084	0.1009	0.5595	-9.2371	83.3965	-13.8882
17	1.9337	-0.2612	-0.4562	1.9638	-0.0784	1.8917	-0.2819	-0.0145	0.0681	-0.2745	0.4183	0.5246	-0.0073	-0.9014	-0.0945	0.4796	-0.0228	-0.1312	0.6396	0.1364	0.5027	-14.5553	79.1598	-12.1953
18	2.2282	1.3427	1.4385	2.3106	0.3219	1.986	-0.0498	0.4786	-0.0581	-0.684	-0.1965	-0.6357	-0.1271	0.4578	0.1231	0.6044	-0.0428	0.0126	0.6317	0.0273	0.5346	3.7835	77.4894	-14.582
19	1.6947	-0.351	-0.1013	1.1787	0.0068	1.3579	-0.1781	-0.2895	-0.2818	0.268	0.1158	-0.1843	0.0067	0.0516	0.0207	0.3696	-0.0721	-0.0558	0.5342	0.1211	0.4862	7.2001	68.0032	-7.1487
20	1.4402	-0.2641	-0.3137	1.9631	0.086	1.9151	-0.251	-0.0681	-0.1944	0.1774	0.2226	0.3524	0.3489	0.0317	0.0085	0.3905	-0.0182	-0.0482	0.4564	0.0971	0.4216	0.0321	64.0312	-5.4936
21	2.577	-0.4827	-0.8644	2.1413	0.5557	2.7859	-0.0268	-0.0664	-0.4048	-0.1644	0.1165	-0.3751	-0.2695	0.5189	-0.0235	0.4782	-0.0675	-0.019	0.5945	0.0594	0.5648	13.7934	62.4986	-9.272
22	1.4357	-0.3282	-0.2951	2.0914	0.3305	1.9916	0.0374	0.4865	0.2482	-0.6668	0.2436	0.1256	0.0579	-0.3095	-0.4164	0.8295	-0.1836	-0.0378	0.5973	0.1392	0.6943	-3.3249	76.9678	-22.1748
23	1.6147	0.0597	-0.6157	1.6712	0.5827	2.3881	-0.1984	0.0022	0.0442	-0.5213	-0.0562	0.5642	0.131	-0.3211	0.1837	0.4624	0.0593	-0.1305	0.5356	0.1038	0.5071	-7.9499	108.9208	6.307
24	2.1897	-0.6871	-0.2483	1.9935	-0.0666	1.3967	0.162	0.4255	0.2794	-0.4773	-0.0773	-0.0216	-0.0607	-0.1174	-0.086	0.4667	0.0115	0.0419	0.5479	0.1349	0.3029	13.5146	106.3102	0.642
25	0.9306	-0.0762	0.103	1.2853	-0.1562	0.4621	0.0232	0.0331	0.0204	0.1012	-0.1513	-0.5654	0.0026	0.0285	0.1218	0.251	-0.0075	-0.0191	0.3084	0.0014	0.2787	62.203	48.4642	43.4595
26	1.6189	0.0242	-0.3923	1.7973	-0.3101	1.565	-0.0415	-0.1096	0.0552	0.0994	-0.0415	-0.171	-0.0734	0.1905	0.0936	0.3044	-0.0302	-0.0878	0.329	-0.0056	0.3068	61.5311	65.4615	45.0082
27	1.7153	-0.1588	0.014	0.474	0.6688	1.3822	0.1027	0.1747	-0.1287	-0.1313	-0.1275	0.1454	-0.1512	-0.1588	0.0442	0.2701	0.0656	-0.0415	0.2461	-0.0149	0.2774	45.2459	59.095	38.7759
28	1.8507	0.375	-0.9556	2.41	-0.2488	3.6354	-0.0018	0.452	-0.2167	0.1128	0.0913	0.6337	0.0645	-0.0664	0.0298	0.2242	0.0678	-0.0991	0.3349	-0.0732	0.4088	34.8171	59.7169	41.909
29	1.0711	1.7035	-1.2638	2.153	-1.3442	0.9198	0.2073	-0.1754	0.0073	0.2039	-0.1753	-0.1024	0.127	0.3115	-0.0777	0.4304	0.0321	-0.0471	0.3724	-0.0319	0.4488	64.846	76.2639	46.4892
30	2.2936	-0.0639	-0.3971	0.6099	0.2088	1.4977	-0.0018	0.0643	0.1268	0.0728	0.0682	-0.1672	0.1259	0.0016	-0.0734	0.2839	0.0053	0.0405	0.2052	-0.0389	0.3001	59.2664	97.7955	29.4055
31	1.7626	0.3763	-0.0098	1.9497	0.4463	3.1343	0.2694	0.0343	0.1038	-0.0834	0.2134	-0.5226	-0.3108	0.6053	-0.2783	0.4014	-0.0618	0.0589	0.3767	-0.0711	0.5277	71.6048	104.9467	29.8058
32	0.5109	-0.1499	1.5851	0.3661	-0.7616	4.4966	-0.2253	0.0103	-0.176	0.0395	-0.2758	-0.2948	0.11	0.7618	0.2091	0.4271	-0.0501	0.0885	0.4412	-0.0345	0.2856	63.6246	100.1493	-4.394
33	0.8958	0.3718	-0.6496	1.0623	-0.9465	0.9398	0.1328	0.0672	0.2304	0.5063	0.131	0.1321	-0.3552	-0.0729	-0.174	0.2837	-0.0648	0.041	0.4025	0.0132	0.3234	53.4487	95.1296	-14.6218
34	1.5605	0.5553	1.3618	1.7957	-0.4724	1.5245	0.2683	0.6425	0.1432	-0.2023	0.0715	-0.1433	0.0788	-0.1649	-0.1384	0.3798	-0.0183	0.0901	0.5241	-0.0312	0.2773	58.1401	85.5268	-17.893
35	2.8382	-0.8512	-0.0036	2.3007	-0.1479	1.7457	-0.3061	-0.3679	-0.0119	0.4136	-0.0191	0.1238	0.4864	0.618	0.226	0.4131	-0.0315	0.0569	0.3977	-0.0146	0.2132	61.1914	91.7556	-5.2
36	1.9619	2.0034	0.2613	2.728	0.5676	1.1998	-0.0878	0.1009	0.0451	-0.2002	0.0879	0.3941	0.0355	-0.4015	0.0625	0.4142	-0.0749	0.0508	0.5153	0.0918	0.3431	42.1656	92.0423	-8.4244
37	1.337	0.2093	-0.0651	1.2123	-0.2444	0.9735	-0.051	-0.2244	-0.1283	-0.0991	0.1146	0.0859	0.3324	-0.2478	0.0034	0.3773	-0.0813	0.0763	0.3825	0.0449	0.3261	42.1217	86.3544	4.7131
38	2.2717	-0.1908	-1.1085	8.2026	-3.6312	3.6722	0.4044	-0.1555	0.3715	0.4696	-0.525	-0.2151	-0.0782	-0.0459	-0.1383	0.4374	-0.0737	0.0468	0.4782	-0.1064	0.2777	52.2586	96.1501	8.6863
39	2.1387	0.2019	-0.9749	1.6007	-0.0375	1.5678	0.9141	-0.2194	1.1009	0.347	-0.1991	0.2573	-0.5675	-0.4	-0.2067	0.4869	0.0359	0.153	0.5219	0.0249	0.5363	38.7665	104.7321	4.8287
40	0.3935	0.1028	-0.3429	5.1978	-2.1841	2.7645	-0.157	-0.8918	-0.1833	0.662	0.005	0.9141	0.4603	-0.1936	-0.2408	0.4636	-0.0749	0.1761	0.4942	0.0372	0.4384	34.0796	86.8141	10.4126
41	-0.2751	-0.5854	-0.2103	3.5381	2.6914	1.597	0.3842	0.0808	0.1333	-0.0326	-0.2793	-0.6007	-0.2204	-0.3454	-0.1175	0.6629	-0.0791	0.1466	0.4895	0.0467	0.5508	52.3253	86.694	-20.2026
42	2.7698	0.0129	-0.8462	1.3949	-0.8784	1.9506	-0.1623	0.2009	0.0038	-0.0514	0.1312	0.0536	-0.0156	0.0321	0.0198	0.4475	-0.0736	-0.0136	0.3693	0.016	0.2719	40.1673	62.322	-26.9916
43	1.614	-0.1008	0.9223	0.1229	0.377	2.4301	0.1524	0.4976	0.1316	-0.5181	0.0681	-0.3832	0.6128	-0.1485	-0.2441	0.6577	0.0127	0.1702	0.6929	0.0728	0.3941	48.4029	59.7972	-38.5397
44	3.112	-0.7574	0.1832	1.2626	0.0043	0.0281	-0.1244	1.1829	0.3943	-0.5992	0.2154	-0.2721	0.8401	0.4744	0.2002	1.024	0.1347	-0.0115	0.4421	-0.0702	0.1819	46.843	30.1813	-17.1663
45	1.3932	0.065	0.1065	0.6509	-0.2503	1.1411	-0.0751	-0.1831	-0.3088	-0.11	-0.0715	-0.2873	0.7652	0.0423	-0.0192	0.6279	0.0033	-0.0208	0.4468	-0.0398	0.3348	46.0873	24.2128	-0.5977
46	3.4323	-0.0575	0.7873	2.9125	-0.1082	1.5387	0.0638	0.1039	0.2335	-0.7166	0.0582	-0.578	0.2326	0.1175	0.0049	0.653	0.0379	0.0456	0.4251	-0.0076	0.3602	48.4577	33.1366	-9.069
47	1.2361	-0.674	-0.06	0.7186	0.0185	1.9593	0.0068	0.0599	0.0308	0.1778	0.1981	0.1067	0.4791	-0.2277	-0.082	0.4925	-0.0521	0.002	0.3973	0.0013	0.292	29.7407	37.7861	-0.8344
48	1.6276	0.0731	0.0101	1.699	-0.1669	2.0515	-0.1179	0.0949	0.2725	0.0419	0.1319	0.1001	-0.2524	-0.1988	-0.0399	0.4864	-0.0384	-0.0456	0.3447	0.0108	0.2623	30.7497	46.3942	-5.1687
49	1.0429	-0.3816	1.5363	-0.085	-0.592	2.7788	0.0407	0.248	-0.2841	-0.3116	0.217	0.114	0.142	-0.0409	-0.1085	0.7147	-0.0716	-0.0843	0.498	-0.066	0.382	44.3468	23.0917	4.0916
50	1.6547	0.3558	-0.0467	3.3786	0.221	1.5189	-0.0658	0.1678	-0.088	-0.489	0.0555	-0.14	0.2021	0.3753	0.0355	0.2987	0.0783	0.0372	0.2941	0.0034	0.2878	60.0132	29.2931	30.3287
51	1.5167	-0.0057	-0.0601	1.7145	0.1157	1.0251	-0.1137	0.1328	0.2123	-0.0765	0.0657	0.249	-0.1061	0.0321	0.026	0.2343	0.034	-0.0283	0.2294	-0.0052	0.2695	39.6233	34.774	34.2913
52	3.0504	-1.3572	-0.6464	1.4613	-0.5881	1.8665	-0.0041	0.1991	-0.4012	-0.2573	-0.1001	0.5113	0.2591	-0.194	0.1569	0.2575	-0.0149	-0.0682	0.2076	-0.0406	0.3351	35.7083	23.895	32.5427
53	1.3765	0.3859	0.4462	2.1413	2.7679	3.5894	0.0605	-0.0162	-0.9133	-0.2674	0.4224	0.2424	0.9917	0.4155	-0.4454	0.9239	0.0692	-0.0749	0.276	-0.0158	0.8749	3.3304	-44.4478	115.2441
54	0.9027	0.0441	-0.0517	2.9507	-0.4515	0.4807	-0.0119	-0.2807	0.117	0.2248	0.2126	0.3815	0.2595	0.2446	-0.1345	0.5091	0.0298	-0.0454	0.612	0.0293	0.4058	9.7759	-24.2583	127.5664
55	4.2303	0.3121	1.5587	0.8952	0.058	3.277	-0.3084	-0.5527	-0.2762	0.6405	0.0941	-0.6023	0.1099	0.6197	0.2466	0.4977	0.0062	-0.0793	0.4601	0.1021	0.4882	15.4138	-5.6092	139.0974
56	1.5185	0.1562	0.0682	2.6793	0.2609	1.3152	-0.1935	-0.0774	0.1483	0.4257	-0.0518	-0.3881	-0.0546	0.4495	0.0764	0.3591	-0.0645	-0.0778	0.5093	0.0767	0.3442	14.8232	9.5465	134.1446
57	2.916	-0.3845	-0.9201	1.162	0.7392	2.3389	-0.3825	0.6065	-0.008	0.2708	0.1016	-0.134	0.5231	-0.0708	0.2725	0.3218	-0.0064	0.0019	0.5869	-0.0102	0.2483	14.2328	-1.9739	129.2408
58	0.4403	-0.4002	0.7064	2.6618	-1.4417	2.1187	0.1755	-0.4806	0.3124	0.5048	-0.2792	0.65	-0.2218	0.0707	0.0954	0.2799	-0.0323	0.0617	0.4232	-0.077	0.3856	-1.2513	9.2249	131.9542
59	0.9602	-0.2176	-0.0732	0.0849	0.2636	0.7551	-0.0049	0.3716	-0.0559	0.1162	-0.1029	0.189	0.0196	-0.1995	-0.0262	0.4116	-0.0251	0.103	0.4732	0.0195	0.4395	-7.7065	3.68	125.5133
60	1.2092	0.0586	-0.2726	1.3254	-0.3244	2.1051	-0.0501	0.5479	0.2006	-0.1712	0.0593	0.0978	-0.2673	-0.3108	-0.062	0.3175	0.0234	0.0078	0.5138	0.0325	0.2699	-3.4276	2.0621	115.5657
61	1.5975	0.5999	-1.8798	0.3809	-0.0421	5.021	0.1479	-0.0633	0.2153	0.0643	0.2388	-0.0352	0.3421	-0.1079	-0.1912	0.3427	-0.0367	-0.0496	0.3985	0.0068	0.4397	-0.3623	-9.9239	125.2894
62	1.6075	0.2779	0.1029	0.801	0.7111	1.3474	0.2062	-0.6253	-0.0837	0.3426	-0.0882	0.2901	0.3692	-0.4928	-0.0974	0.4	-0.1145	0.0389	0.4406	0.0199	0.3842	-12.758	-5.1462	134.9992
63	1.3041	0.8482	-0.9241	0.6718	-1.1601	5.4865	-0.0193	0.332	0.0815	-0.2677	0.1421	0.5143	-0.0941	-0.3322	-0.0064	0.2876	-0.0231	-0.0123	0.6437	0.0496	0.3142	-14.7706	2.2604	117.2415
64	0.4817	1.0743	-0.1609	1.3434	-0.1051	0.6981	0.1605	-0.1852	-0.0181	0.4179	-0.2863	-0.1749	0.1171	0.2935	0.1134	0.5058	0.0599	-0.0151	0.5488	0.1218	0.4374	13.8276	15.6072	134.4672
65	2.2306	-0.2525	0.1702	1.1951	-0.0983	1.3612	-0.058	-0.3722	0.1384	0.1818	-0.0239	-0.106	-0.1047	0.0693	0.0862	0.3708	-0.0624	-0.0816	0.4465	-0.0121	0.3246	12.6584	39.0066	118.2416
66	1.08	-0.4	0.0027	0.7783	0.0393	1.5145	-0.1615	-0.1745	0.2988	0.3976	0.2408	-0.0515	-0.3198	0.6283	0.0542	0.5743	-0.0652	-0.1273	0.6646	-0.0745	0.4094	24.6103	44.447	115.6952
67	1.4631	-0.1239	-0.0113	1.4994	0.2464	1.462	-0.1026	0.0682	0.1057	-0.3962	0.1349	-0.2696	-0.336	0.2708	-0.0247	0.4191	-0.0755	0.0474	0.3147	-0.024	0.2714	15.8885	37.0497	76.933
68	1.4668	-0.1525	-0.3693	1.445	-0.2331	1.9439	-0.121	-0.1385	0.0196	0.1176	-0.1067	0.237	-0.0281	-0.0423	0.189	0.3228	-0.0587	-0.0479	0.3185	-0.0106	0.304	1.33	34.549	91.3743
69	2.2005	-1.163	0.0072	2.3953	0.0557	1.8955	-0.2018	-0.1345	0.1496	-0.3577	-0.2023	0.191	-0.1608	-0.2276	0.0973	0.4353	0.0381	-0.0685	0.3614	-0.0017	0.391	-8.7606	38.4274	93.0739
70	0.0646	-0.508	-0.2875	0.8932	1.2105	1.7115	0.1339	-0.0464	0.1581	-0.276	-0.0675	-0.415	-0.5396	0.1314	-0.0376	0.3661	0.018	0.0162	0.3287	-0.0028	0.4161	14.1821	19.661	63.6744
71	1.8445	-0.05	-0.0772	0.429	0.2067	0.7864	-0.0885	-0.0382	-0.0312	-0.0256	0.0473	-0.0699	-0.0129	0.0388	0.0366	0.2503	0.0255	0.0051	0.2396	-0.0201	0.2749	3.4515	6.4875	57.1637
72	2.0803	0.4075	-1.2937	0.8707	-0.1732	1.5962	0.1774	0.1988	0.1521	0.0088	0.0052	0.0848	-0.2645	-0.1642	-0.1777	0.2571	0.0783	0.0001	0.2147	-0.033	0.3107	-6.03	13.6537	56.8131
73	1.3922	0.4398	0.2282	3.4124	-0.2696	2.1329	-0.1383	0.4843	-0.1109	-0.6021	0.2179	-0.3219	-0.0431	0.1578	-0.0792	0.3412	0.0309	0.0924	0.3685	0.0313	0.3286	12.1172	4.9871	47.4235
74	1.2934	0.2743	-1.2363	3.9445	-0.6139	2.0562	-0.3899	0.2204	-0.09	-0.8255	0.5921	-0.8032	0.4325	0.1358	0.117	0.323	0.0035	0.0531	0.3387	-0.0174	0.3891	7.2699	-24.5887	66.3164
75	2.3711	-0.1595	0.0566	0.9719	-0.1934	1.7129	-0.0616	0.0475	-0.434	0.1259	0.1679	-0.2598	0.6389	0.2629	-0.0118	0.4656	0.0464	-0.0573	0.2672	-0.0344	0.4354	3.4508	-31.7542	82.0029
76	3.3147	-0.0735	0.6396	2.5007	0.02	2.1099	-0.125	0.1078	0.2253	-0.0621	0.0197	-0.9185	0.1194	0.0499	-0.0462	0.2079	-0.0057	-0.0467	0.2276	-0.0006	0.3788	7.2811	-22.2455	74.7969
77	1.1241	-0.0652	-0.4874	1.4224	0.8796	2.7343	-0.0352	-0.2886	0.0609	-0.0052	-0.257	-0.039	0.3331	-0.8116	0.1408	0.2808	-0.0547	-0.0228	0.3534	-0.0281	0.3706	-10.8778	-24.2442	80.9658
78	1.0951	-0.1808	-0.4719	1.5332	-0.2105	1.1725	0.0516	-0.3194	0.354	0.205	0.0169	0.1352	-0.1183	-0.1883	-0.0424	0.2275	-0.0104	-0.002	0.2908	-0.0774	0.3102	-9.9033	-9.9708	81.3679
79	2.7271	1.4438	1.5113	3.4465	2.5074	4.2301	-0.222	0.4483	0.2788	-0.4877	0.0623	0.305	-0.4659	-0.6084	0.0402	0.2786	0.0164	0.0322	0.3248	0.0148	0.3758	-3.1211	-10.3319	68.3586
80	2.9682	-0.3459	-0.1752	2.1411	-0.4644	1.7969	0.3761	-0.0335	-0.152	-0.2526	-0.1809	0.0379	0.0644	-0.4617	0.0424	0.258	-0.0146	-0.049	0.3051	-0.0024	0.3682	-18.2912	-16.1755	65.9345
81	1.8404	1.5859	1.5506	3.7781	2.0626	2.7589	-0.1241	-0.2249	0.7633	0.8043	-0.2734	0.4876	-0.1109	-0.6377	0.0749	0.2368	0.1154	-0.062	0.3778	-0.1132	0.5368	-18.0171	-4.2929	81.1454
82	1.2161	-0.391	0.2494	0.8843	0.3937	2.7827	0.0572	0.0126	-0.3491	0.0271	0.1148	-0.0226	0.4937	0.3728	-0.1638	0.462	-0.0689	-0.0485	0.3489	-0.056	0.3964	-5.587	-31.3795	78.4769
83	2.3334	-0.5665	0.1772	1.5434	0.5245	1.994	0.0831	-0.007	-0.3318	0.052	0.0149	0.0647	0.5152	0.1028	-0.1187	0.4533	0.0354	-0.0541	0.2994	0.0385	0.2872	-1.9033	-29.9683	114.2336
84	1.1875	0.4717	0.2635	1.7176	0.7396	1.2458	0.0192	-0.5295	-0.353	0.6577	0.098	0.2258	-0.0405	-0.6166	0.0229	0.7074	0.0893	0.2947	0.4621	0.0725	0.4874	46.0236	9.8723	147.588
85	1.3359	0.3528	-0.5005	1.2406	-0.3116	1.8151	0.0556	0.0946	0.0473	0.1266	0.185	0.1272	0.1571	-0.6009	-0.1742	0.3021	-0.0345	0.0269	0.4224	0.0677	0.2979	41.1575	-18.2129	117.0695
86	0.7846	1.1413	1.5335	1.6382	2.1943	3.0065	0.0213	-0.0265	0.2783	-0.5143	-0.0542	-0.0702	0.1084	-0.1958	-0.0432	0.4517	-0.0277	0.0072	0.4612	0.105	0.4133	39.2762	-13.2686	100.1236
87	2.2796	-0.1682	0.6934	1.8788	-0.0619	1.389	-0.1558	0.0387	0.1477	-0.1758	0.201	0.2473	-0.0779	-0.4586	-0.0531	0.3234	-0.127	0.0056	0.478	0.0706	0.3785	38.3741	-14.9198	111.1711
88	0.7266	0.1996	-0.4666	1.3388	-0.3043	3.7845	0.0929	0.1996	-0.4062	-0.0403	0.0949	-0.1761	0.6598	-0.1312	-0.0422	0.3575	0.0046	0.0085	0.3759	0.0011	0.4152	56.1791	-18.9071	107.944
89	0.8225	-0.1017	0.1688	1.0848	0.0976	0.9875	0.0841	-0.0078	0.2704	-0.0838	0.0412	-0.0525	-0.0628	0.0173	-0.0513	0.3282	-0.0374	0.1008	0.3967	0.0107	0.3759	60.5552	-5.27	111.4375
90	2.4078	-0.1401	0.6787	1.8287	-0.1763	1.3921	0.0192	-0.1707	0.1487	-0.0459	0.0703	-0.2322	0.1638	-0.0116	-0.1489	0.333	-0.0338	0.039	0.3772	-0.0039	0.4371	65.5528	-12.0586	120.2317
91	0.3042	0.2722	1.1079	0.2567	-0.3318	3.5168	0.1226	-0.0323	0.067	0.1754	-0.1618	0.1368	0.5669	0.2894	-0.0504	0.5302	0.0905	0.0764	0.5962	0.1708	0.5404	41.5245	-22.08	101.3759
92	2.0661	-0.2681	-0.2862	1.1788	-0.0948	0.8995	-0.009	-0.0169	-0.187	0.0306	-0.0337	0.0725	0.0726	-0.0847	0.0425	0.2483	-0.0077	-0.0041	0.2353	0.0102	0.3603	47.7645	-14.6199	74.0982
93	1.3644	-0.2765	-0.3199	1.7423	0.6356	1.0191	0.0367	0.3179	-0.2524	-0.1054	0.0483	0.4002	0.306	-0.0691	-0.0621	0.315	-0.0693	-0.0748	0.2887	-0.024	0.3968	37.0745	-12.7971	66.6513
94	1.4938	-0.0327	0.107	0.9799	0.2954	1.6865	-0.0795	-0.0514	0.1501	-0.0062	0.0546	0.1318	-0.1236	0.0228	0.0205	0.2029	0.0321	-0.0033	0.1946	-0.0131	0.2664	50.6703	25.064	62.5522
95	1.8957	-0.6785	-0.3666	1.9598	0.068	1.4377	-0.027	-0.1719	-0.102	0.0603	0.0587	-0.0531	0.0635	0.1753	0.0046	0.1368	0.0106	-0.0106	0.1927	0.0042	0.2173	64.6406	10.1392	71.5455
96	-0.5368	0.0817	0.6073	1.002	1.1852	1.8777	-0.0626	0.2274	-0.0116	-0.0749	-0.2168	0.011	-0.2605	-0.6192	0.1438	0.3876	0.0668	-0.0491	0.5039	-0.0825	0.4051	52.1485	43.5402	74.4425
97	2.2348	-0.4369	-0.1547	1.4175	-0.0476	1.6742	-0.1204	0.1201	0.2366	-0.0006	-0.0034	-0.044	-0.2303	0.0121	0.0801	0.3734	0.0123	-0.0615	0.2231	-0.0148	0.2526	66.2943	52.3312	83.3706
98	0.9125	0.2226	-0.3346	1.4499	-0.0761	2.9738	0.0559	0.4832	0.5311	-0.2813	-0.3674	0.1832	-0.501	-0.7584	0.0775	0.7648	0.3343	-0.123	0.5172	0.0471	0.4354	54.5095	64.1931	82.2843
99	0.4615	-0.1948	-0.4117	1.3651	0.1145	2.8865	0.2923	0.0791	-0.1094	-0.035	-0.2933	0.1586	-0.6333	-0.4675	0.0455	0.4601	0.0777	0.0207	0.3569	-0.0539	0.4244	54.5495	55.732	117.3138
100	2.1495	0.547	-0.5473	1.4021	-0.2379	1.2214	0.0562	-0.1808	-0.0065	0.1752	-0.1114	0.1045	-0.0799	-0.1025	0.0316	0.3162	0.008	-0.0297	0.318	-0.0293	0.2105	63.8563	41.1654	120.3174
101	1.711	0.1801	-0.3305	1.4932	0.2102	0.8697	-0.0514	0.1664	-0.0456	-0.1816	0.0483	-0.1706	-0.0078	0.0387	-0.0095	0.3951	0.0303	-0.0055	0.332	-0.0206	0.246	72.7184	40.571	107.942
102	1.338	0.9846	-1.5665	2.2047	-0.807	1.1961	0.1719	-0.4141	-0.0135	0.3897	-0.2249	0.3035	-0.2946	0.2418	0.0275	0.483	-0.0276	-0.0386	0.4619	0.014	0.4321	57.1784	38.2447	133.3538
103	1.7206	-0.2715	-0.2249	1.3404	0.0198	0.3516	-0.0973	0.0593	0.0581	0.1887	0.1137	0.069	-0.1683	0.0623	-0.0025	0.4275	-0.0152	0.1165	0.4342	0.0383	0.2971	56.7726	10.4191	138.188

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	CHAIN A AND (RESID 4 THROUGH 30 )
2	X-RAY DIFFRACTION	2	CHAIN A AND (RESID 31 THROUGH 81 )
3	X-RAY DIFFRACTION	3	CHAIN A AND (RESID 82 THROUGH 116 )
4	X-RAY DIFFRACTION	4	CHAIN A AND (RESID 117 THROUGH 134 )
5	X-RAY DIFFRACTION	5	CHAIN A AND (RESID 135 THROUGH 186 )
6	X-RAY DIFFRACTION	6	CHAIN A AND (RESID 187 THROUGH 226 )
7	X-RAY DIFFRACTION	7	CHAIN A AND (RESID 227 THROUGH 252 )
8	X-RAY DIFFRACTION	8	CHAIN B AND (RESID 4 THROUGH 51 )
9	X-RAY DIFFRACTION	9	CHAIN B AND (RESID 52 THROUGH 252 )
10	X-RAY DIFFRACTION	10	CHAIN C AND (RESID 3 THROUGH 30 )
11	X-RAY DIFFRACTION	11	CHAIN C AND (RESID 31 THROUGH 163 )
12	X-RAY DIFFRACTION	12	CHAIN C AND (RESID 164 THROUGH 252 )
13	X-RAY DIFFRACTION	13	CHAIN D AND (RESID 4 THROUGH 51 )
14	X-RAY DIFFRACTION	14	CHAIN D AND (RESID 52 THROUGH 252 )
15	X-RAY DIFFRACTION	15	CHAIN E AND (RESID 4 THROUGH 51 )
16	X-RAY DIFFRACTION	16	CHAIN E AND (RESID 52 THROUGH 81 )
17	X-RAY DIFFRACTION	17	CHAIN E AND (RESID 82 THROUGH 116 )
18	X-RAY DIFFRACTION	18	CHAIN E AND (RESID 117 THROUGH 134 )
19	X-RAY DIFFRACTION	19	CHAIN E AND (RESID 135 THROUGH 186 )
20	X-RAY DIFFRACTION	20	CHAIN E AND (RESID 187 THROUGH 226 )
21	X-RAY DIFFRACTION	21	CHAIN E AND (RESID 227 THROUGH 252 )
22	X-RAY DIFFRACTION	22	CHAIN F AND (RESID 4 THROUGH 30 )
23	X-RAY DIFFRACTION	23	CHAIN F AND (RESID 31 THROUGH 125 )
24	X-RAY DIFFRACTION	24	CHAIN F AND (RESID 126 THROUGH 252 )
25	X-RAY DIFFRACTION	25	CHAIN G AND (RESID 4 THROUGH 81 )
26	X-RAY DIFFRACTION	26	CHAIN G AND (RESID 82 THROUGH 134 )
27	X-RAY DIFFRACTION	27	CHAIN G AND (RESID 135 THROUGH 226 )
28	X-RAY DIFFRACTION	28	CHAIN G AND (RESID 227 THROUGH 252 )
29	X-RAY DIFFRACTION	29	CHAIN H AND (RESID 4 THROUGH 51 )
30	X-RAY DIFFRACTION	30	CHAIN H AND (RESID 52 THROUGH 252 )
31	X-RAY DIFFRACTION	31	CHAIN I AND (RESID 4 THROUGH 30 )
32	X-RAY DIFFRACTION	32	CHAIN I AND (RESID 31 THROUGH 51 )
33	X-RAY DIFFRACTION	33	CHAIN I AND (RESID 52 THROUGH 69 )
34	X-RAY DIFFRACTION	34	CHAIN I AND (RESID 70 THROUGH 95 )
35	X-RAY DIFFRACTION	35	CHAIN I AND (RESID 96 THROUGH 116 )
36	X-RAY DIFFRACTION	36	CHAIN I AND (RESID 117 THROUGH 134 )
37	X-RAY DIFFRACTION	37	CHAIN I AND (RESID 135 THROUGH 202 )
38	X-RAY DIFFRACTION	38	CHAIN I AND (RESID 203 THROUGH 216 )
39	X-RAY DIFFRACTION	39	CHAIN I AND (RESID 217 THROUGH 237 )
40	X-RAY DIFFRACTION	40	CHAIN I AND (RESID 238 THROUGH 252 )
41	X-RAY DIFFRACTION	41	CHAIN J AND (RESID 4 THROUGH 51 )
42	X-RAY DIFFRACTION	42	CHAIN J AND (RESID 52 THROUGH 252 )
43	X-RAY DIFFRACTION	43	CHAIN K AND (RESID 2 THROUGH 30 )
44	X-RAY DIFFRACTION	44	CHAIN K AND (RESID 31 THROUGH 51 )
45	X-RAY DIFFRACTION	45	CHAIN K AND (RESID 52 THROUGH 95 )
46	X-RAY DIFFRACTION	46	CHAIN K AND (RESID 96 THROUGH 116 )
47	X-RAY DIFFRACTION	47	CHAIN K AND (RESID 117 THROUGH 163 )
48	X-RAY DIFFRACTION	48	CHAIN K AND (RESID 164 THROUGH 252 )
49	X-RAY DIFFRACTION	49	CHAIN L AND (RESID 3 THROUGH 51 )
50	X-RAY DIFFRACTION	50	CHAIN L AND (RESID 52 THROUGH 125 )
51	X-RAY DIFFRACTION	51	CHAIN L AND (RESID 126 THROUGH 202 )
52	X-RAY DIFFRACTION	52	CHAIN L AND (RESID 203 THROUGH 252 )
53	X-RAY DIFFRACTION	53	CHAIN M AND (RESID 5 THROUGH 19 )
54	X-RAY DIFFRACTION	54	CHAIN M AND (RESID 20 THROUGH 36 )
55	X-RAY DIFFRACTION	55	CHAIN M AND (RESID 37 THROUGH 51 )
56	X-RAY DIFFRACTION	56	CHAIN M AND (RESID 52 THROUGH 95 )
57	X-RAY DIFFRACTION	57	CHAIN M AND (RESID 96 THROUGH 116 )
58	X-RAY DIFFRACTION	58	CHAIN M AND (RESID 117 THROUGH 134 )
59	X-RAY DIFFRACTION	59	CHAIN M AND (RESID 135 THROUGH 163 )
60	X-RAY DIFFRACTION	60	CHAIN M AND (RESID 164 THROUGH 202 )
61	X-RAY DIFFRACTION	61	CHAIN M AND (RESID 203 THROUGH 216 )
62	X-RAY DIFFRACTION	62	CHAIN M AND (RESID 217 THROUGH 237 )
63	X-RAY DIFFRACTION	63	CHAIN M AND (RESID 238 THROUGH 252 )
64	X-RAY DIFFRACTION	64	CHAIN N AND (RESID 4 THROUGH 51 )
65	X-RAY DIFFRACTION	65	CHAIN N AND (RESID 52 THROUGH 252 )
66	X-RAY DIFFRACTION	66	CHAIN O AND (RESID 5 THROUGH 36 )
67	X-RAY DIFFRACTION	67	CHAIN O AND (RESID 37 THROUGH 134 )
68	X-RAY DIFFRACTION	68	CHAIN O AND (RESID 135 THROUGH 226 )
69	X-RAY DIFFRACTION	69	CHAIN O AND (RESID 227 THROUGH 252 )
70	X-RAY DIFFRACTION	70	CHAIN P AND (RESID 4 THROUGH 81 )
71	X-RAY DIFFRACTION	71	CHAIN P AND (RESID 82 THROUGH 186 )
72	X-RAY DIFFRACTION	72	CHAIN P AND (RESID 187 THROUGH 252 )
73	X-RAY DIFFRACTION	73	CHAIN Q AND (RESID 4 THROUGH 30 )
74	X-RAY DIFFRACTION	74	CHAIN Q AND (RESID 31 THROUGH 51 )
75	X-RAY DIFFRACTION	75	CHAIN Q AND (RESID 52 THROUGH 95 )
76	X-RAY DIFFRACTION	76	CHAIN Q AND (RESID 96 THROUGH 116 )
77	X-RAY DIFFRACTION	77	CHAIN Q AND (RESID 117 THROUGH 134 )
78	X-RAY DIFFRACTION	78	CHAIN Q AND (RESID 135 THROUGH 202 )
79	X-RAY DIFFRACTION	79	CHAIN Q AND (RESID 203 THROUGH 216 )
80	X-RAY DIFFRACTION	80	CHAIN Q AND (RESID 217 THROUGH 237 )
81	X-RAY DIFFRACTION	81	CHAIN Q AND (RESID 238 THROUGH 252 )
82	X-RAY DIFFRACTION	82	CHAIN R AND (RESID 5 THROUGH 36 )
83	X-RAY DIFFRACTION	83	CHAIN R AND (RESID 37 THROUGH 252 )
84	X-RAY DIFFRACTION	84	CHAIN S AND (RESID 4 THROUGH 30 )
85	X-RAY DIFFRACTION	85	CHAIN S AND (RESID 31 THROUGH 69 )
86	X-RAY DIFFRACTION	86	CHAIN S AND (RESID 70 THROUGH 81 )
87	X-RAY DIFFRACTION	87	CHAIN S AND (RESID 82 THROUGH 116 )
88	X-RAY DIFFRACTION	88	CHAIN S AND (RESID 117 THROUGH 134 )
89	X-RAY DIFFRACTION	89	CHAIN S AND (RESID 135 THROUGH 210 )
90	X-RAY DIFFRACTION	90	CHAIN S AND (RESID 211 THROUGH 252 )
91	X-RAY DIFFRACTION	91	CHAIN T AND (RESID 3 THROUGH 51 )
92	X-RAY DIFFRACTION	92	CHAIN T AND (RESID 52 THROUGH 252 )
93	X-RAY DIFFRACTION	93	CHAIN U AND (RESID 5 THROUGH 36 )
94	X-RAY DIFFRACTION	94	CHAIN U AND (RESID 37 THROUGH 134 )
95	X-RAY DIFFRACTION	95	CHAIN U AND (RESID 135 THROUGH 251 )
96	X-RAY DIFFRACTION	96	CHAIN V AND (RESID 4 THROUGH 81 )
97	X-RAY DIFFRACTION	97	CHAIN V AND (RESID 82 THROUGH 252 )
98	X-RAY DIFFRACTION	98	CHAIN W AND (RESID 3 THROUGH 30 )
99	X-RAY DIFFRACTION	99	CHAIN W AND (RESID 31 THROUGH 51 )
100	X-RAY DIFFRACTION	100	CHAIN W AND (RESID 52 THROUGH 186 )
101	X-RAY DIFFRACTION	101	CHAIN W AND (RESID 187 THROUGH 252 )
102	X-RAY DIFFRACTION	102	CHAIN X AND (RESID 4 THROUGH 51 )
103	X-RAY DIFFRACTION	103	CHAIN X AND (RESID 52 THROUGH 252 )

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