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- PDB-4co3: Structure of PII signaling protein GlnZ from Azospirillum brasile... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4co3 | |||||||||
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Title | Structure of PII signaling protein GlnZ from Azospirillum brasilense in complex with adenosine triphosphate | |||||||||
![]() | PII-LIKE PROTEIN PZ | |||||||||
![]() | SIGNALING PROTEIN / GLNK-LIKE | |||||||||
Function / homology | ![]() regulation of nitrogen utilization / enzyme regulator activity / ATP binding / metal ion binding / cytosol Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Truan, D. / Li, X.-D. / Winkler, F.K. | |||||||||
![]() | ![]() Title: Structure and thermodynamics of effector molecule binding to the nitrogen signal transduction PII protein GlnZ from Azospirillum brasilense. Authors: Truan, D. / Bjelic, S. / Li, X.D. / Winkler, F.K. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 176.6 KB | Display | ![]() |
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PDB format | ![]() | 136.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 18.3 KB | Display | |
Data in CIF | ![]() | 28 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4cnySC ![]() 4cnzC ![]() 4co0C ![]() 4co1C ![]() 4co2C ![]() 4co4C ![]() 4co5C S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 12287.109 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.93 Å3/Da / Density % sol: 36.29 % / Description: NONE |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 0.1M MES PH 6.5, 40% PEG 200, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 3, 2009 Details: VERTICALLY COLLIMATING MIRROR FOLLOWED BY A BARTELS MONOCHROMATOR AND A TOROIDAL MIRROR |
Radiation | Monochromator: BARTELS MONOCHROMATOR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection twin | Operator: h,-h-k,-l / Fraction: 0.53 |
Reflection | Resolution: 1.05→32.48 Å / Num. obs: 84996 / % possible obs: 99 % / Observed criterion σ(I): 2 / Redundancy: 5.21 % / Rmerge(I) obs: 0.09 / Net I/σ(I): 2.3 |
Reflection shell | Resolution: 1.05→1.1 Å / Redundancy: 3.58 % / Rmerge(I) obs: 0.88 / Mean I/σ(I) obs: 2 / % possible all: 97.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 4CNY Resolution: 1.05→32.477 Å / σ(F): 2 / Phase error: 21.1 / Stereochemistry target values: TWIN_LSQ_F
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.05→32.477 Å
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Refine LS restraints |
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LS refinement shell |
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