+Open data
-Basic information
Entry | Database: PDB / ID: 4chh | ||||||
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Title | N-terminal domain of yeast PIH1p | ||||||
Components | PROTEIN INTERACTING WITH HSP90 1 | ||||||
Keywords | CHAPERONE / R2TP / TAH1 | ||||||
Function / homology | Function and homology information R2TP complex / box C/D snoRNP assembly / regulation of cell size / RNA splicing / mRNA processing / rRNA processing / protein folding / protein stabilization / ribonucleoprotein complex / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | SACCHAROMYCES CEREVISIAE (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.03 Å | ||||||
Authors | Roe, S.M. / Pal, M. | ||||||
Citation | Journal: Structure / Year: 2014 Title: Structural Basis for Phosphorylation-Dependent Recruitment of Tel2 to Hsp90 by Pih1. Authors: Pal, M. / Morgan, M. / Phelps, S.E. / Roe, S.M. / Parry-Morris, S. / Downs, J.A. / Polier, S. / Pearl, L.H. / Prodromou, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4chh.cif.gz | 128.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4chh.ent.gz | 103.8 KB | Display | PDB format |
PDBx/mmJSON format | 4chh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ch/4chh ftp://data.pdbj.org/pub/pdb/validation_reports/ch/4chh | HTTPS FTP |
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-Related structure data
Related structure data | 4cguC 4cgvC 4cgwC 4cktC 4cseC 4cv4C C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 21452.855 Da / Num. of mol.: 2 / Fragment: N-TERMINUS, RESIDUES 1-185 Source method: isolated from a genetically manipulated source Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast) Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P38768 #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47 % / Description: NONE |
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Crystal grow | pH: 7 / Details: pH 7 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 1.03666 |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 22, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.03666 Å / Relative weight: 1 |
Reflection | Resolution: 2.03→48.29 Å / Num. obs: 23130 / % possible obs: 97.9 % / Observed criterion σ(I): 0 / Redundancy: 3 % / Biso Wilson estimate: 27.41 Å2 / Rmerge(I) obs: 0.07 / Net I/σ(I): 9.7 |
Reflection shell | Resolution: 2.03→2.08 Å / Redundancy: 2.3 % / Rmerge(I) obs: 0.36 / Mean I/σ(I) obs: 2.2 / % possible all: 87.6 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: IN-HOUSE MOUSE PIH Resolution: 2.03→47.66 Å / Cor.coef. Fo:Fc: 0.9251 / Cor.coef. Fo:Fc free: 0.9072 / SU R Cruickshank DPI: 0.179 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.189 / SU Rfree Blow DPI: 0.169 / SU Rfree Cruickshank DPI: 0.166
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Displacement parameters | Biso mean: 37.13 Å2
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Refine analyze | Luzzati coordinate error obs: 0.228 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.03→47.66 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.03→2.12 Å / Total num. of bins used: 12
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