|Entry||Database: PDB / ID: 4c4p|
|Title||Crystal Structure of Wild-Type Rab11 Complexed to FIP2|
|Keywords||PROTEIN TRANSPORT / EFFECTOR / VESICLE TRAFFICKING / ENDOSOMES|
|Function / homology|
Function and homology information
TRAM-dependent toll-like receptor 4 signaling pathway / regulation of multivesicular body size / postsynaptic recycling endosome / establishment of protein localization to organelle / plasma membrane to endosome transport / establishment of vesicle localization / exosomal secretion / regulated exocytosis / melanosome transport / astral microtubule organization ...TRAM-dependent toll-like receptor 4 signaling pathway / regulation of multivesicular body size / postsynaptic recycling endosome / establishment of protein localization to organelle / plasma membrane to endosome transport / establishment of vesicle localization / exosomal secretion / regulated exocytosis / melanosome transport / astral microtubule organization / VxPx cargo-targeting to cilium / amyloid-beta clearance by transcytosis / neurotransmitter receptor transport, endosome to postsynaptic membrane / regulation of vesicle-mediated transport / myosin V binding / RAB geranylgeranylation / multivesicular body assembly / insulin secretion involved in cellular response to glucose stimulus / establishment of protein localization to membrane / protein localization to cell surface / TBC/RABGAPs / mitotic metaphase plate congression / phagocytic cup / syntaxin binding / positive regulation of epithelial cell migration / cleavage furrow / exocytosis / mitotic spindle assembly / centriolar satellite / phagocytosis / establishment of cell polarity / phagocytic vesicle / Vasopressin regulates renal water homeostasis via Aquaporins / G protein activity / transport vesicle / Anchoring of the basal body to the plasma membrane / small monomeric GTPase / positive regulation of G2/M transition of mitotic cell cycle / multivesicular body / centriole / vesicle-mediated transport / Translocation of SLC2A4 (GLUT4) to the plasma membrane / regulation of cytokinesis / cell projection / protein localization to plasma membrane / trans-Golgi network / cytoplasmic vesicle membrane / recycling endosome / small GTPase binding / spindle pole / recycling endosome membrane / neuron projection development / positive regulation of GTPase activity / microtubule binding / cytoplasmic vesicle / vesicle / endosome / GTPase activity / axon / centrosome / glutamatergic synapse / intracellular membrane-bounded organelle / GTP binding / protein kinase binding / Golgi apparatus / protein homodimerization activity / protein-containing complex / extracellular exosome / nucleoplasm / identical protein binding / cytosol
Similarity search - Function
Rab11-family interacting protein class I / Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #2440 / FIP-RBD domain profile. / FIP domain / FIP-RBD, C-terminal domain superfamily / Rab-binding domain FIP-RBD / small GTPase Rab1 family profile. / C2 domain / Protein kinase C conserved region 2 (CalB) / C2 domain ...Rab11-family interacting protein class I / Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #2440 / FIP-RBD domain profile. / FIP domain / FIP-RBD, C-terminal domain superfamily / Rab-binding domain FIP-RBD / small GTPase Rab1 family profile. / C2 domain / Protein kinase C conserved region 2 (CalB) / C2 domain / C2 domain profile. / C2 domain superfamily / Rho (Ras homology) subfamily of Ras-like small GTPases / Small GTPase / Ras family / Single alpha-helices involved in coiled-coils or other helix-helix interfaces / Small GTP-binding protein domain / P-loop containing nucleotide triphosphate hydrolases / Up-down Bundle / P-loop containing nucleoside triphosphate hydrolase / Rossmann fold / 3-Layer(aba) Sandwich / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / Ras-related protein Rab-11A / Rab11 family-interacting protein 2
Similarity search - Component
|Biological species||HOMO SAPIENS (human)|
|Method||X-RAY DIFFRACTION / SYNCHROTRON / OTHER / Resolution: 2 Å|
|Authors||Sultana, A. / Khan, A.R.|
|Citation||Journal: Biochim.Biophys.Acta / Year: 2013|
Title: Structural and Functional Analysis of Fip2 Binding to the Endosome-Localised Rab25 Gtpase
Authors: Lall, P. / Horgan, C.P. / Oda, S. / Franklin, E. / Sultana, A. / Hanscomb, S.R. / Mccaffrey, M.W. / Khan, A.R.
|Structure viewer||Molecule: |
Downloads & links
A: RAS-RELATED PROTEIN RAB-11A
B: RAB11 FAMILY-INTERACTING PROTEIN 2
|#1: Protein|| |
Mass: 19515.906 Da / Num. of mol.: 1 / Fragment: RESIDUES 1-173
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P62491
|#2: Protein|| |
Mass: 12289.915 Da / Num. of mol.: 1 / Fragment: RAB-BINDING DOMAIN, RESIDUES 410-512
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: Q7L804
|#3: Chemical|| ChemComp-GNP / |
|#4: Chemical|| ChemComp-MG / |
|#5: Water|| ChemComp-HOH / |
|Sequence details||N-TERMINALLY AND C-TERMINALLY TRUNCATED RAB11A, WITH A FEW NON-NATIVE RESIDUES AT N-TERMINUS FROM ...N-TERMINALLY|
|Experiment||Method: X-RAY DIFFRACTION / Number of used crystals: 1|
|Crystal||Density Matthews: 2.13 Å3/Da / Density % sol: 42.38 % / Description: NONE|
|Diffraction||Mean temperature: 100 K|
|Diffraction source||Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-2 / Wavelength: 0.976|
|Detector||Type: ADSC CCD / Detector: CCD|
|Radiation||Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray|
|Radiation wavelength||Wavelength: 0.976 Å / Relative weight: 1|
|Reflection||Resolution: 2→40 Å / Num. obs: 18989 / % possible obs: 99.9 % / Observed criterion σ(I): 1 / Redundancy: 8.9 % / Rmerge(I) obs: 0.05 / Net I/σ(I): 26.7|
|Software||Name: PHENIX / Version: (PHENIX.REFINE) / Classification: refinement|
|Refinement||Method to determine structure: OTHER|
Starting model: NONE
Resolution: 2→27.955 Å / SU ML: 0.22 / σ(F): 1.36 / Phase error: 19.53 / Stereochemistry target values: ML
|Solvent computation||Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL|
|Refinement step||Cycle: LAST / Resolution: 2→27.955 Å|
|Refine LS restraints|
|LS refinement shell|
|Refinement TLS params.||Method: refined / Origin x: 26.9827 Å / Origin y: 8.3308 Å / Origin z: 21.774 Å|
|Refinement TLS group||Selection details: ALL|
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