Mass: 9029.109 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: THE FIRST RESIDUE (S) OF THE CONSTRUCT IS A CLONING ARTEFACT. THE FIRST RESIDUE OF THE MATURE PROTEIN AFTER SIGNAL PEPTIDE CLEAVAGE (A) IS MISSING. THIS IS A CHIMERIC PROTEIN OF NC2 (UNIPROT ...Details: THE FIRST RESIDUE (S) OF THE CONSTRUCT IS A CLONING ARTEFACT. THE FIRST RESIDUE OF THE MATURE PROTEIN AFTER SIGNAL PEPTIDE CLEAVAGE (A) IS MISSING. THIS IS A CHIMERIC PROTEIN OF NC2 (UNIPROT Q7S3P5, RESIDUES 19-80 AND 91-97) AND EAS (UNIPROT Q04571, RESIDUES 88-105) Source: (gene. exp.) NEUROSPORA CRASSA (fungus) / Strain: 4R74A, 4R74A Description: ARTIFICIAL CHIMERIC PROTEIN, SEE DETAILS IN MOLECULE SECTION. Plasmid: PHUE / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q7S3P5, UniProt: Q04571
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
H-H NOESY
2
2
2
T1
2
3
2
T2
2
4
2
HETERONOE
NMR details
Text: THE STRUCTURE WAS DETERMINED USING H-H NOESY WITH UNLABELLED NCHI2 TOGETHER WITH PREDICTED TORSION ANGLE RESTRAINTS FROM 1H, 13C, AND 15N CHEMICAL SHIFTS.
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Sample preparation
Details
Solution-ID
Contents
1
90% H2O/10% D2O
2
90% H2O/10% D2O
Sample conditions
Conditions-ID
Ionic strength
pH
Pressure (kPa)
Temperature (K)
1
50.0mM
2.5
1.0atm
318.0K
2
50.0mM
2.5
1.0atm
318.0K
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NMR measurement
NMR spectrometer
Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz
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