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Yorodumi- PDB-4bsn: Crystal structure of the Nuclear Export Receptor CRM1 (exportin-1... -
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-Basic information
Entry | Database: PDB / ID: 4bsn | ||||||
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Title | Crystal structure of the Nuclear Export Receptor CRM1 (exportin-1) lacking the C-terminal helical extension at 4.1A | ||||||
Components | EXPORTIN-1 | ||||||
Keywords | PROTEIN TRANSPORT / HEAT REPEAT PROTEIN / IMPORTIN-BETA SUPERFAMILY / NUCLEOCYTOPLASMIC TRANSPORT OF PROTEIN AND RNP CARGOES | ||||||
Function / homology | Function and homology information HuR (ELAVL1) binds and stabilizes mRNA / annulate lamellae / regulation of proteasomal ubiquitin-dependent protein catabolic process / regulation of centrosome duplication / nuclear export signal receptor activity / regulation of protein export from nucleus / Rev-mediated nuclear export of HIV RNA / NEP/NS2 Interacts with the Cellular Export Machinery / nucleocytoplasmic transport / Maturation of hRSV A proteins ...HuR (ELAVL1) binds and stabilizes mRNA / annulate lamellae / regulation of proteasomal ubiquitin-dependent protein catabolic process / regulation of centrosome duplication / nuclear export signal receptor activity / regulation of protein export from nucleus / Rev-mediated nuclear export of HIV RNA / NEP/NS2 Interacts with the Cellular Export Machinery / nucleocytoplasmic transport / Maturation of hRSV A proteins / protein localization to nucleus / ribosomal large subunit export from nucleus / Estrogen-dependent nuclear events downstream of ESR-membrane signaling / ribosomal small subunit export from nucleus / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / mRNA export from nucleus / ribosomal subunit export from nucleus / Cyclin A/B1/B2 associated events during G2/M transition / Cajal body / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Resolution of Sister Chromatid Cohesion / NPAS4 regulates expression of target genes / protein export from nucleus / Downregulation of TGF-beta receptor signaling / Deactivation of the beta-catenin transactivating complex / RHO GTPases Activate Formins / Heme signaling / MAPK6/MAPK4 signaling / kinetochore / small GTPase binding / Separation of Sister Chromatids / ribosome biogenesis / nuclear envelope / nuclear membrane / ribonucleoprotein complex / intracellular membrane-bounded organelle / nucleolus / protein-containing complex / RNA binding / nucleoplasm / membrane / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 4.1 Å | ||||||
Authors | Dian, C. / Bernaudat, F. / Langer, K. / Oliva, M.F. / Fornerod, M. / Schoehn, G. / Muller, C.W. / Petosa, C. | ||||||
Citation | Journal: Structure / Year: 2013 Title: Structure of a Truncation Mutant of the Nuclear Export Factor Crm1 Provides Insights Into the Auto-Inhibitory Role of its C-Terminal Helix. Authors: Dian, C. / Bernaudat, F. / Langer, K. / Oliva, M.F. / Fornerod, M. / Schoehn, G. / Muller, C.W. / Petosa, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4bsn.cif.gz | 141.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4bsn.ent.gz | 108.2 KB | Display | PDB format |
PDBx/mmJSON format | 4bsn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4bsn_validation.pdf.gz | 428 KB | Display | wwPDB validaton report |
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Full document | 4bsn_full_validation.pdf.gz | 434.3 KB | Display | |
Data in XML | 4bsn_validation.xml.gz | 25.1 KB | Display | |
Data in CIF | 4bsn_validation.cif.gz | 33.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bs/4bsn ftp://data.pdbj.org/pub/pdb/validation_reports/bs/4bsn | HTTPS FTP |
-Related structure data
Related structure data | 4bsmC 3gb8S C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 118863.172 Da / Num. of mol.: 1 Fragment: LACKS THE C-TERMINAL HELICAL EXTENSION, RESIDUE 1-1032 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PQE60 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): TG1 / References: UniProt: O14980 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.15 Å3/Da / Density % sol: 70.38 % / Description: NONE |
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Crystal grow | pH: 7.5 Details: BY MIXING EQUAL VOLUMES OF PROTEIN (8-10 MG/ML) AND RESERVOIR SOLUTION 0.8 M SODIUM ACETATE, 50 MM TRIS PH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-2 / Wavelength: 0.8726 |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8726 Å / Relative weight: 1 |
Reflection | Resolution: 4.1→30 Å / Num. obs: 15484 / % possible obs: 99.5 % / Observed criterion σ(I): 2 / Redundancy: 4.9 % / Rmerge(I) obs: 0.12 / Net I/σ(I): 8 |
Reflection shell | Resolution: 4.1→4.35 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.5 / Mean I/σ(I) obs: 2.6 / % possible all: 94.5 |
-Processing
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 3GB8 Resolution: 4.1→48.049 Å / SU ML: 0.53 / σ(F): 1.38 / Phase error: 34.73 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.8 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 4.1→48.049 Å
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Refine LS restraints |
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LS refinement shell |
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