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- PDB-4bsm: Crystal structure of the Nuclear Export Receptor CRM1 (exportin-1... -
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Basic information
Entry | Database: PDB / ID: 4bsm | ||||||
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Title | Crystal structure of the Nuclear Export Receptor CRM1 (exportin-1) lacking the C-terminal helical extension at 4.5A | ||||||
![]() | EXPORTIN-1 | ||||||
![]() | PROTEIN TRANSPORT / HEAT REPEAT PROTEIN / IMPORTIN-BETA SUPERFAMILY / NUCLEOCYTOPLASMIC TRANSPORT OF PROTEIN AND RNP CARGOES | ||||||
Function / homology | ![]() HuR (ELAVL1) binds and stabilizes mRNA / annulate lamellae / regulation of proteasomal ubiquitin-dependent protein catabolic process / regulation of centrosome duplication / nuclear export signal receptor activity / regulation of protein export from nucleus / Rev-mediated nuclear export of HIV RNA / NEP/NS2 Interacts with the Cellular Export Machinery / nucleocytoplasmic transport / Maturation of hRSV A proteins ...HuR (ELAVL1) binds and stabilizes mRNA / annulate lamellae / regulation of proteasomal ubiquitin-dependent protein catabolic process / regulation of centrosome duplication / nuclear export signal receptor activity / regulation of protein export from nucleus / Rev-mediated nuclear export of HIV RNA / NEP/NS2 Interacts with the Cellular Export Machinery / nucleocytoplasmic transport / Maturation of hRSV A proteins / protein localization to nucleus / ribosomal large subunit export from nucleus / Estrogen-dependent nuclear events downstream of ESR-membrane signaling / ribosomal small subunit export from nucleus / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / mRNA export from nucleus / ribosomal subunit export from nucleus / Cyclin A/B1/B2 associated events during G2/M transition / Cajal body / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Resolution of Sister Chromatid Cohesion / NPAS4 regulates expression of target genes / protein export from nucleus / Downregulation of TGF-beta receptor signaling / Deactivation of the beta-catenin transactivating complex / RHO GTPases Activate Formins / Heme signaling / MAPK6/MAPK4 signaling / kinetochore / small GTPase binding / Separation of Sister Chromatids / ribosome biogenesis / nuclear envelope / nuclear membrane / ribonucleoprotein complex / intracellular membrane-bounded organelle / nucleolus / protein-containing complex / RNA binding / nucleoplasm / membrane / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Dian, C. / Bernaudat, F. / Langer, K. / Oliva, M.F. / Fornerod, M. / Schoehn, G. / Muller, C.W. / Petosa, C. | ||||||
![]() | ![]() Title: Structure of a Truncation Mutant of the Nuclear Export Factor Crm1 Provides Insights Into the Auto-Inhibitory Role of its C-Terminal Helix. Authors: Dian, C. / Bernaudat, F. / Langer, K. / Oliva, M.F. / Fornerod, M. / Schoehn, G. / Muller, C.W. / Petosa, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 170.2 KB | Display | ![]() |
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PDB format | ![]() | 133.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 427.6 KB | Display | ![]() |
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Full document | ![]() | 443 KB | Display | |
Data in XML | ![]() | 31.7 KB | Display | |
Data in CIF | ![]() | 42.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4bsnC ![]() 3gb8S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 118863.172 Da / Num. of mol.: 1 / Fragment: RESIDUES 1-1032 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.26 Å3/Da / Density % sol: 71.15 % / Description: NONE |
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Crystal grow | pH: 8 Details: BY MIXING EQUAL VOLUMES OF PROTEIN (8-10 MG/ML) AND RESERVOIR SOLUTION 0.5 M DISODIUM TARTRATE, 50 MM TRIS PH 8.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Dec 5, 2009 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9754 Å / Relative weight: 1 |
Reflection | Resolution: 4.5→48.59 Å / Num. obs: 12112 / % possible obs: 99.9 % / Observed criterion σ(I): 2 / Redundancy: 3.6 % / Biso Wilson estimate: 145.78 Å2 / Rmerge(I) obs: 0.15 / Net I/σ(I): 7.1 |
Reflection shell | Resolution: 4.5→4.74 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.6 / Mean I/σ(I) obs: 2.4 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3GB8 Resolution: 4.5→48.589 Å / SU ML: 0.5 / σ(F): 1.33 / Phase error: 26.69 / Stereochemistry target values: ML Details: RESIDUES 3-19 AND 932-1017 WERE MODELED AS POLYALANINE OR POLYGLYCINE DUE TO AMBIGUOUS DENSITY
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Solvent computation | Shrinkage radii: 0.8 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 4.5→48.589 Å
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Refine LS restraints |
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LS refinement shell |
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