PROTOPORPHYRIN IX CONTAINING FE (HEM): THIOLATE BOND BETWEEN FE AND C 354 SULFATE ION (SO4): PART ...PROTOPORPHYRIN IX CONTAINING FE (HEM): THIOLATE BOND BETWEEN FE AND C 354 SULFATE ION (SO4): PART OF CRYSTALLIZATION CONDITIONS GLYCEROL (GOL): CRYO-PROTECTANT [(3R,4S,5S,7R,9E,11R,12R)-12-ETHYL-3,5,7,11-TETRAMETHYL-2, 8-BIS(OXIDANYLIDENE)-1-OXACYCLODODEC-9-EN-4-YL] 3-(DIMETHYLAMINO)PROPANOATE (QLE): SYNTHETIC SUBSTRATE ANALOG
配列の詳細
FIRST 20 RESIDUES INCLUDING 6X HIS-TAG AND THROMBIN CLEAVAGE SITE ARE FROM THE CLONING VECTOR ...FIRST 20 RESIDUES INCLUDING 6X HIS-TAG AND THROMBIN CLEAVAGE SITE ARE FROM THE CLONING VECTOR PET28A D50N MUTATION IS ENGINEERED
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.13 Å3/Da / 溶媒含有率: 42.33 % / 解説: NONE
結晶化
pH: 5.5 / 詳細: 2 M AMMONIUM SULFATE, 0.1 M BIS TRIS, PH 5.5
解像度: 1.84→61.24 Å / Cor.coef. Fo:Fc: 0.963 / Cor.coef. Fo:Fc free: 0.932 / SU B: 3.522 / SU ML: 0.105 / 交差検証法: THROUGHOUT / ESU R: 0.151 / ESU R Free: 0.148 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
Rfactor
反射数
%反射
Selection details
Rfree
0.2242
3187
5 %
RANDOM
Rwork
0.16586
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obs
0.16887
59932
94.38 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: MASK