+Open data
-Basic information
Entry | Database: PDB / ID: 4axs | ||||||
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Title | Structure of Carbamate Kinase from Mycoplasma penetrans | ||||||
Components | CARBAMATE KINASE | ||||||
Keywords | OXIDOREDUCTASE | ||||||
Function / homology | Function and homology information | ||||||
Biological species | MYCOPLASMA PENETRANS (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | ||||||
Authors | Gallego, P. / Planell, R. / Benach, J. / Querol, E. / PerezPons, J.A. / Reverter, D. | ||||||
Citation | Journal: Plos One / Year: 2012 Title: Structural Characterization of the Enzymes Composing the Arginine Deiminase Pathway in Mycoplasma Penetrans. Authors: Gallego, P. / Planell, R. / Benach, J. / Querol, E. / Perez-Pons, J.A. / Reverter, D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4axs.cif.gz | 68.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4axs.ent.gz | 49.3 KB | Display | PDB format |
PDBx/mmJSON format | 4axs.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ax/4axs ftp://data.pdbj.org/pub/pdb/validation_reports/ax/4axs | HTTPS FTP |
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-Related structure data
Related structure data | 4amuC 4anfC 4e4jC 1e19S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 35562.539 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) MYCOPLASMA PENETRANS (bacteria) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q8EVF4 | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.9 Å3/Da / Density % sol: 35.15 % / Description: NONE |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-2 / Wavelength: 0.8726 |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8726 Å / Relative weight: 1 |
Reflection | Resolution: 2.38→44.86 Å / Num. obs: 11222 / % possible obs: 97.7 % / Observed criterion σ(I): 2 / Redundancy: 4.8 % / Biso Wilson estimate: 45.29 Å2 / Rmerge(I) obs: 0.07 / Net I/σ(I): 11.8 |
Reflection shell | Resolution: 2.38→2.51 Å / Redundancy: 4.5 % / Rmerge(I) obs: 0.39 / Mean I/σ(I) obs: 2.5 / % possible all: 92.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1E19 Resolution: 2.5→44.86 Å / SU ML: 0.38 / σ(F): 1.35 / Phase error: 30.52 / Stereochemistry target values: ML / Details: RESIDUES 134 TO 143 AND 151 TO 156 ARE DISORDERED
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 62.096 Å2 / ksol: 0.356 e/Å3 | ||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.5→44.86 Å
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Refine LS restraints |
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LS refinement shell |
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