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- PDB-4ats: Structure of the ORF273 protein from the Acidianus two-tailed virus -
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Open data
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Basic information
Entry | Database: PDB / ID: 4ats | ||||||
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Title | Structure of the ORF273 protein from the Acidianus two-tailed virus | ||||||
![]() | STRUCTURAL PROTEIN ORF273 | ||||||
![]() | VIRAL PROTEIN / ARCHAEAL VIRUS / EXTREMOPHILES / BICAUDAVIRUS / HYPER-THERMOSTABILITY | ||||||
Function / homology | : / Acidianus two-tailed virus, ORF273 protein / virion component / Structural protein ORF273![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Felisberto-Rodrigues, C. / Ortiz-Lombardia, M. | ||||||
![]() | ![]() Title: Crystal Structure of Atv(Orf273), a New Fold for a Thermo-and Acido-Stable Protein from the Acidianus Two-Tailed Virus. Authors: Felisberto-Rodrigues, C. / Blangy, S. / Goulet, A. / Vestergaard, G. / Cambillau, C. / Garrett, R.A. / Ortiz-Lombardia, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 109.5 KB | Display | ![]() |
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PDB format | ![]() | 90.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 430 KB | Display | ![]() |
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Full document | ![]() | 431.3 KB | Display | |
Data in XML | ![]() | 10.1 KB | Display | |
Data in CIF | ![]() | 12.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 34365.508 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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Sequence details | THE PROTEIN INCLUDES AN N-TERMINAL HIS-TAG |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.97 Å3/Da / Density % sol: 37.7 % / Description: NONE |
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 7.5 Details: PROTEIN AT 5.5 MG/ML IN 20 MM HEPES (PH 7.5) AND 100 MM NACL WAS MIXED TO 5%-15% PEG 8000, 0.2 M MGCL2, 0.1 M TRIS(PH 7-8). CRYSTALS WERE OBTAINED BY THE HANGING-DROP VAPOUR-DIFFUSION METHOD |
-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jul 29, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.181 Å / Relative weight: 1 |
Reflection | Resolution: 3.85→101.01 Å / Num. obs: 2841 / % possible obs: 99.4 % / Observed criterion σ(I): -3 / Redundancy: 11.02 % / Biso Wilson estimate: 63.92 Å2 / Rmerge(I) obs: 0.14 / Net I/σ(I): 20.57 |
Reflection shell | Resolution: 3.85→3.93 Å / Redundancy: 11.63 % / Rmerge(I) obs: 0.61 / Mean I/σ(I) obs: 5.51 / % possible all: 99.5 |
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Processing
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Refinement | Method to determine structure: OTHER / Resolution: 3.85→46.92 Å / Cor.coef. Fo:Fc: 0.8648 / Cor.coef. Fo:Fc free: 0.8404 / Cross valid method: THROUGHOUT / σ(F): 0 / SU Rfree Blow DPI: 0.95 Details: RESIDUES 47-57 WERE NOT VISIBLE IN THE MAPS. REFINEMENT NOTE 1: IDEAL-DIST CONTACT TERM CONTACT SETUP. ALL ATOMS HAVE CCP4 ATOM TYPE FROM LIBRARY.
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Displacement parameters | Biso mean: 141.21 Å2
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Refine analyze | Luzzati coordinate error obs: 1.379 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.85→46.92 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.85→4.3 Å / Total num. of bins used: 5
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Refinement TLS params. | Method: refined / Origin x: 27.2436 Å / Origin y: 8.3855 Å / Origin z: -11.0454 Å
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