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- PDB-4am0: Structure of Dengue virus strain 4 DIII in complex with Fab 2H12 -

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Basic information

Entry
Database: PDB / ID: 4am0
TitleStructure of Dengue virus strain 4 DIII in complex with Fab 2H12
Components
  • ENVELOPE PROTEIN,Viral envelope
  • FAB 2H12, HEAVY CHAIN
  • FAB 2H12, LIGHT CHAIN
KeywordsVIRAL PROTEIN / ANTIBODY / NEUTRALISATION
Function / homology
Function and homology information


: / host cell mitochondrion / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of host TYK2 activity / flavivirin / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT2 activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / : / viral capsid / nucleoside-triphosphate phosphatase / protein complex oligomerization ...: / host cell mitochondrion / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of host TYK2 activity / flavivirin / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT2 activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / : / viral capsid / nucleoside-triphosphate phosphatase / protein complex oligomerization / double-stranded RNA binding / monoatomic ion channel activity / mRNA (guanine-N7)-methyltransferase / methyltransferase cap1 / clathrin-dependent endocytosis of virus by host cell / mRNA (nucleoside-2'-O-)-methyltransferase activity / mRNA 5'-cap (guanine-N7-)-methyltransferase activity / RNA helicase activity / host cell endoplasmic reticulum membrane / membrane => GO:0016020 / host cell perinuclear region of cytoplasm / protein dimerization activity / RNA helicase / induction by virus of host autophagy / RNA-directed RNA polymerase / viral RNA genome replication / RNA-dependent RNA polymerase activity / serine-type endopeptidase activity / fusion of virus membrane with host endosome membrane / viral envelope / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / host cell nucleus / structural molecule activity / virion attachment to host cell / endoplasmic reticulum membrane / virion membrane / extracellular region / ATP binding / metal ion binding
Similarity search - Function
Immunoglobulin-like - #350 / Flavivirus capsid protein C superfamily / Flavivirus non-structural protein NS2B / Genome polyprotein, Flavivirus / Flavivirus non-structural protein NS4A / Flavivirus non-structural protein NS2B / Flavivirus capsid protein C / Flavivirus non-structural protein NS4B / mRNA cap 0/1 methyltransferase / Flavivirus capsid protein C ...Immunoglobulin-like - #350 / Flavivirus capsid protein C superfamily / Flavivirus non-structural protein NS2B / Genome polyprotein, Flavivirus / Flavivirus non-structural protein NS4A / Flavivirus non-structural protein NS2B / Flavivirus capsid protein C / Flavivirus non-structural protein NS4B / mRNA cap 0/1 methyltransferase / Flavivirus capsid protein C / Flavivirus non-structural protein NS4B / Flavivirus non-structural protein NS4A / Flavivirus NS2B domain profile. / mRNA cap 0 and cap 1 methyltransferase (EC 2.1.1.56 and EC 2.1.1.57) domain profile. / Flavivirus non-structural protein NS2A / Flavivirus non-structural protein NS2A / Flavivirus NS3, petidase S7 / Peptidase S7, Flavivirus NS3 serine protease / Flavivirus NS3 protease (NS3pro) domain profile. / Envelope glycoprotein M, flavivirus / Flavivirus envelope glycoprotein M / RNA-directed RNA polymerase, flavivirus / Flavivirus RNA-directed RNA polymerase, fingers and palm domains / Flavivirus non-structural Protein NS1 / Flavivirus non-structural protein NS1 / Envelope glycoprotein M superfamily, flavivirus / Flavivirus polyprotein propeptide / Flavivirus polyprotein propeptide superfamily / Flavivirus polyprotein propeptide / Flaviviral glycoprotein E, central domain, subdomain 1 / Flaviviral glycoprotein E, central domain, subdomain 2 / Flavivirus envelope glycoprotein E, Stem/Anchor domain / Flavivirus glycoprotein E, immunoglobulin-like domain / Flavivirus envelope glycoprotein E, Stem/Anchor domain superfamily / Flavivirus glycoprotein, immunoglobulin-like domain / Flavivirus glycoprotein central and dimerisation domain / Flavivirus glycoprotein, central and dimerisation domains / Ribosomal RNA methyltransferase, FtsJ domain / FtsJ-like methyltransferase / Flavivirus/Alphavirus glycoprotein, immunoglobulin-like domain superfamily / Flavivirus glycoprotein, central and dimerisation domain superfamily / Flaviviral glycoprotein E, dimerisation domain / DEAD box, Flavivirus / Flavivirus DEAD domain / helicase superfamily c-terminal domain / Immunoglobulin E-set / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / RNA-directed RNA polymerase, catalytic domain / RdRp of positive ssRNA viruses catalytic domain profile. / S-adenosyl-L-methionine-dependent methyltransferase superfamily / Immunoglobulins / Peptidase S1, PA clan, chymotrypsin-like fold / Peptidase S1, PA clan / DNA/RNA polymerase superfamily / Immunoglobulin-like / Sandwich / P-loop containing nucleoside triphosphate hydrolase / Mainly Beta
Similarity search - Domain/homology
Genome polyprotein / Envelope protein E
Similarity search - Component
Biological speciesHOMO SAPIENS (human)
MUS MUSCULUS (house mouse)
DENGUE VIRUS
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.02 Å
AuthorsMidgley, C.M. / Flanagan, A. / Mongkolsapaya, J. / Grimes, J.M. / Screaton, G.R.
CitationJournal: J.Immunol. / Year: 2012
Title: Structural Analysis of a Dengue Cross-Reactive Antibody Complexed with Envelope Domain III Reveals the Molecular Basis of Cross-Reactivity.
Authors: Midgley, C.M. / Flanagan, A. / Tran, H.B. / Dejnirattisai, W. / Chawansuntati, K. / Jumnainsong, A. / Wongwiwat, W. / Duangchinda, T. / Mongkolsapaya, J. / Grimes, J.M. / Screaton, G.R.
History
DepositionMar 6, 2012Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jun 6, 2012Provider: repository / Type: Initial release
Revision 1.1Apr 22, 2015Group: Derived calculations / Other
Revision 1.2Nov 20, 2019Group: Other / Source and taxonomy / Category: entity_src_nat / pdbx_database_status / Item: _pdbx_database_status.status_code_sf
Revision 1.3Dec 4, 2019Group: Source and taxonomy / Structure summary / Category: entity / entity_src_gen / entity_src_nat / Item: _entity.src_method

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: FAB 2H12, HEAVY CHAIN
B: FAB 2H12, LIGHT CHAIN
C: FAB 2H12, HEAVY CHAIN
D: FAB 2H12, LIGHT CHAIN
E: FAB 2H12, HEAVY CHAIN
F: FAB 2H12, LIGHT CHAIN
H: FAB 2H12, HEAVY CHAIN
L: FAB 2H12, LIGHT CHAIN
Q: ENVELOPE PROTEIN,
R: ENVELOPE PROTEIN,
S: ENVELOPE PROTEIN,
T: ENVELOPE PROTEIN,


Theoretical massNumber of molelcules
Total (without water)230,66512
Polymers230,66512
Non-polymers00
Water0
1
A: FAB 2H12, HEAVY CHAIN
B: FAB 2H12, LIGHT CHAIN
S: ENVELOPE PROTEIN,


Theoretical massNumber of molelcules
Total (without water)57,6663
Polymers57,6663
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3300 Å2
ΔGint-24.2 kcal/mol
Surface area19100 Å2
MethodPISA
2
C: FAB 2H12, HEAVY CHAIN
D: FAB 2H12, LIGHT CHAIN
T: ENVELOPE PROTEIN,


Theoretical massNumber of molelcules
Total (without water)57,6663
Polymers57,6663
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3300 Å2
ΔGint-23.4 kcal/mol
Surface area19050 Å2
MethodPISA
3
E: FAB 2H12, HEAVY CHAIN
F: FAB 2H12, LIGHT CHAIN
Q: ENVELOPE PROTEIN,


Theoretical massNumber of molelcules
Total (without water)57,6663
Polymers57,6663
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3300 Å2
ΔGint-23.5 kcal/mol
Surface area19280 Å2
MethodPISA
4
H: FAB 2H12, HEAVY CHAIN
L: FAB 2H12, LIGHT CHAIN
R: ENVELOPE PROTEIN,


Theoretical massNumber of molelcules
Total (without water)57,6663
Polymers57,6663
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3300 Å2
ΔGint-23.5 kcal/mol
Surface area19280 Å2
MethodPISA
Unit cell
Length a, b, c (Å)69.930, 92.800, 96.590
Angle α, β, γ (deg.)118.55, 90.33, 104.05
Int Tables number1
Space group name H-MP1

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Components

#1: Antibody
FAB 2H12, HEAVY CHAIN


Mass: 23035.535 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human), (gene. exp.) MUS MUSCULUS (house mouse)
Production host: MUS MUSCULUS (house mouse)
#2: Antibody
FAB 2H12, LIGHT CHAIN


Mass: 23558.031 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human), (gene. exp.) MUS MUSCULUS (house mouse)
Production host: MUS MUSCULUS (house mouse)
#3: Protein
ENVELOPE PROTEIN, / Viral envelope / E GLYCOPROTEIN


Mass: 11072.658 Da / Num. of mol.: 4 / Fragment: DOMAIN III, RESIDUES 295-395
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) DENGUE VIRUS / Strain: 4 / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: Q7TGC6, UniProt: Q58HT7*PLUS

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.29 Å3/Da / Density % sol: 46.3 % / Description: NONE
Crystal growpH: 7.5 / Details: 20% PEG 3350, 0.2 M POTASSIUM THIOCYNATE, pH 7.5

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.975
DetectorType: ADSC CCD / Detector: CCD
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.975 Å / Relative weight: 1
ReflectionResolution: 3.02→56 Å / Num. obs: 38472 / % possible obs: 96 % / Observed criterion σ(I): 0 / Redundancy: 2 % / Biso Wilson estimate: 56.48 Å2 / Rmerge(I) obs: 0.11 / Net I/σ(I): 8.1
Reflection shellResolution: 3.02→3.1 Å / Redundancy: 2 % / Rmerge(I) obs: 0.48 / Mean I/σ(I) obs: 1.8 / % possible all: 96.2

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Processing

Software
NameVersionClassification
BUSTER2.11.2refinement
XDSdata reduction
SCALAdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.02→41.99 Å / Cor.coef. Fo:Fc: 0.8481 / Cor.coef. Fo:Fc free: 0.7801 / Cross valid method: THROUGHOUT / σ(F): 0 / SU Rfree Blow DPI: 0.47
Details: REFINEMENT NOTES 1:IDEAL-DIST CONTACT SETUP. ALL ATOMS HAVE CCP4 ATOM TYPE FROM LIBRARY. LSSR (-AUTONCS).
RfactorNum. reflection% reflectionSelection details
Rfree0.2522 1926 5.01 %RANDOM
Rwork0.196 ---
obs0.1988 38469 95.74 %-
Displacement parametersBiso mean: 44.69 Å2
Baniso -1Baniso -2Baniso -3
1-19.9275 Å2-0.6354 Å2-2.1855 Å2
2---15.5409 Å2-9.3245 Å2
3----4.3866 Å2
Refine analyzeLuzzati coordinate error obs: 0.554 Å
Refinement stepCycle: LAST / Resolution: 3.02→41.99 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms15830 0 0 0 15830
Refine LS restraints
Refine-IDTypeDev idealNumberRestraint functionWeight
X-RAY DIFFRACTIONt_bond_d0.0116224HARMONIC2
X-RAY DIFFRACTIONt_angle_deg1.2522087HARMONIC2
X-RAY DIFFRACTIONt_dihedral_angle_d5345SINUSOIDAL2
X-RAY DIFFRACTIONt_incorr_chiral_ct
X-RAY DIFFRACTIONt_pseud_angle
X-RAY DIFFRACTIONt_trig_c_planes333HARMONIC2
X-RAY DIFFRACTIONt_gen_planes2348HARMONIC5
X-RAY DIFFRACTIONt_it16224HARMONIC20
X-RAY DIFFRACTIONt_nbd
X-RAY DIFFRACTIONt_omega_torsion2.89
X-RAY DIFFRACTIONt_other_torsion22.33
X-RAY DIFFRACTIONt_improper_torsion
X-RAY DIFFRACTIONt_chiral_improper_torsion2216SEMIHARMONIC5
X-RAY DIFFRACTIONt_sum_occupancies
X-RAY DIFFRACTIONt_utility_distance
X-RAY DIFFRACTIONt_utility_angle
X-RAY DIFFRACTIONt_utility_torsion
X-RAY DIFFRACTIONt_ideal_dist_contact17863SEMIHARMONIC4
LS refinement shellResolution: 3.02→3.1 Å / Total num. of bins used: 19
RfactorNum. reflection% reflection
Rfree0.3439 156 5.47 %
Rwork0.2441 2696 -
all0.2496 2852 -
obs--95.74 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
14.13551.0907-2.26732.63321.87224.2765-0.0258-0.0045-0.01720.03750.06540.16010.22540.0616-0.0396-0.22370.01980.01070.00460.1552-0.004329.4486-42.4574-24.2169
21.06880.5249-0.62931.4995-1.5650.93560.0091-0.066-0.05080.09960.0251-0.08930.00640.047-0.03430.1038-0.10560.1093-0.03340.0125-0.0630.4152-63.774-1.3185
31.9545-1.1312-2.3695-1.1727-0.08631.3341-0.00540.0179-0.00550.01690.0297-0.04520.02160.0629-0.02440.08250.0309-0.0230.05890.0546-0.118735.2324-61.91936.3903
41.76830.7711-0.53481.7948-1.3065-0.1723-0.08930.0415-0.3494-0.29180.02070.12830.26960.10240.0687-0.0350.04330.044-0.0711-0.00150.011832.5038-63.2815-28.1863
50.8863-0.39871.53483.1417-0.2551-0.7726-0.01860.061-0.04980.05890.01050.0430.01070.0160.00810.0064-0.03750.1309-0.0730.08220.040120.547-79.5623-7.6236
63.7714-0.4829-0.81761.3490.13121.18060.0030.0141-0.15580.18860.01360.0103-0.0239-0.0073-0.01660.1145-0.08850.1073-0.09640.1569-0.097422.3142-76.5573-3.1641
74.98042.3365-1.38343.56920.18031.3534-0.10160.16650.0535-0.1104-0.00880.0090.09950.12690.1103-0.28160.0504-0.01890.15620.0838-0.0543-8.8541-70.866826.233
81.4282-0.0453-0.5757-0.44890.39412.23990.04420.02870.0264-0.1881-0.00650.0656-0.02490.0044-0.0377-0.0487-0.18910.0138-0.00750.01230.0782-7.3715-61.6507-3.8021
90.3828-0.13070.0576-0.22930.04770.20860.00270.0025-0.0031-0.007-0.0070.0013-0.0057-0.00470.0043-0.01590.0023-0.05190.0833-0.0064-0.0045-10.2671-55.2889-16.7215
101.595-3.84230.85350.24040.59970.6441-0.04030.0384-0.0271-0.05590.0517-0.09640.08640.001-0.01130.08670.14380.0120.0067-0.0126-0.0492-6.0827-92.058210.7347
111.67070.51161.89910.82282.40215.9878-0.09570.13330.0364-0.09430.1592-0.01830.1777-0.0201-0.06350.20640.01830.1244-0.14980.0531-0.1323-12.6795-89.085117.3916
124.1458-0.0505-0.0542-0.31410.81661.1116-0.01060.12940.018-0.1449-0.036-0.20950.13020.120.04660.0324-0.06760.10230.0410.1128-0.15641.3242-71.7534-13.8921
134.4349-2.33470.99763.0862-0.37053.21120.0454-0.03140.00240.14250.1283-0.076-0.1199-0.0435-0.1737-0.2348-0.00780.00720.12610.0603-0.0898-8.5887-27.3019-16.9574
140.5015-0.3169-0.9660.6893-0.49483.8902-0.0111-0.04170.03490.1061-0.0418-0.09510.0609-0.07190.0529-0.08650.0703-0.0480.1842-0.0203-0.1021-7.3503-36.265211.363
154.3318-2.13780.4277-1.9437-0.16651.1828-0.02440.02290.03640.0435-0.00680.10840.0232-0.00470.03120.0419-0.09790.06390.08130.045-0.0641-13.4652-43.846316.921
161.72672.1884-1.6930.77860.91315.0372-0.0138-0.12620.2296-0.01490.0677-0.004-0.4138-0.1261-0.0540.10330.20430.0498-0.04830.0436-0.1066-12.9438-9.1474-6.4706
170.59711.46130.813-0.31290.62552.690.0465-0.0659-0.06050.0395-0.032-0.0391-0.1094-0.0085-0.0145-0.01380.149-0.03270.0780.0149-0.1098-4.1244-27.002921.7984
185.18650.0505-1.55631.12920.38590.45170.04-0.1005-0.0270.011-0.0746-0.1140.0686-0.03890.03460.08830.0583-0.090.0377-0.0423-0.24730.8039-28.410524.6694
190.2243-0.95922.38822.04971.05263.7099-0.03290.1719-0.0256-0.07280.02490.1488-0.01430.08230.008-0.29260.00130.02750.03450.15010.140328.9688-57.993134.3784
203.1604-1.8175-1.01830.1176-1.1353-0.9139-0.00410.0333-0.0149-0.088-0.07930.03670.0005-0.01750.0834-0.10880.0177-0.07910.05570.07070.075728.1485-50.92232.0825
211.131-0.45451.30442.3549-2.46261.550.01670.19410.0554-0.1923-0.0538-0.1089-0.24010.07110.0372-0.1061-0.05320.0580.14610.1208-0.067930.2594-33.57849.4137
220.7252-0.36510.07471.9439-1.31393.0274-0.1860.10310.24010.23990.0476-0.0182-0.17060.23060.1384-0.1156-0.046-0.0078-0.09830.02960.041530.5707-34.547537.9374
230.78154.1173-1.40070.69330.77581.76280.0108-0.01980.0174-0.04740-0.0046-0.0860.0008-0.01080.04060.0116-0.0746-0.07260.0793-0.008919.5454-23.839217.7822
240.5706-0.66220.00850.8476-1.0551.0302-0.02260.05260.09470.0320.0205-0.0291-0.0233-0.06060.00210.0534-0.0007-0.07730.01760.0606-0.025517.4858-16.65099.0492
251.2538-2.1272-1.02121.13710.76320.9281-0.0015-0.04710.059-0.02510.0314-0.0326-0.0289-0.0491-0.0299-0.06020.08060.04320.0961-0.0067-0.0041-3.3568-13.5886-37.3891
260.6196-0.9103-0.4083-0.07421.56512.77690.00910.0147-0.0172-0.02040.0232-0.0749-0.0125-0.0382-0.0323-0.11230.10020.06660.0681-0.0090.00410.4818-9.059-40.7729
270.87911.2804-2.3807-0.91530.34622.3136-0.00920.02040.02570.00490.0187-0.0235-0.00460.0185-0.0095-0.0153-0.06910.00930.1445-0.0637-0.071-8.4861-2.6198-36.0737
283.0334-1.38961.9421.556-0.96373.1372-0.02460.00660.077-0.0185-0.02950.01590.0572-0.03680.0541-0.24240.0217-0.03780.1760.145-0.0008-5.2481-10.2141-39.984
291.4925-0.010.34030.7102-0.4835-0.6478-0.0036-0.00250.0102-0.0015-0.04060.0595-0.0158-0.02290.0442-0.1225-0.1052-0.13430.0263-0.0090.112623.8364-67.356657.101
301.320.5982-1.856-1.28441.07871.616-0.0147-0.00120.0561-0.071-0.04560.08730.07660.03150.0603-0.084-0.05930.03680.0694-0.01180.059221.0088-68.07162.9404
310.57270.6725-1.8353-0.42070.65881.7453-0.0231-0.04180.04870.03230.042-0.02530.0811-0.1053-0.019-0.19040.0926-0.01410.09150.04080.096219.1653-62.541159.5702
321.8741-2.18181.3454-1.3643-0.39722.1486-0.024-0.01560.0260.06410.03210.01010.05770.0198-0.0082-0.18250.0751-0.02040.10450.08190.097732.3636-69.614862.6683
331.26580.0295-1.36661.3633-1.00380.44740.01820.0049-0.0464-0.04440.0231-0.0323-0.04360.0226-0.0413-0.08110.06920.06210.04330.12650.066122.4068-30.209-47.9107
340.2283-0.543-0.00211.9995-0.8962-0.6482-0.00430.0292-0.014-0.0181-0.00460.07460.0020.07670.0089-0.19720.06150.07430.0340.0380.109823.9733-32.1825-57.5936
35-0.0743-0.19570.10332.6419-0.0515-0.9082-0.00340.02230.0053-0.01480.00220.0526-0.01470.00280.00110.0693-0.08430.0401-0.0055-0.1093-0.007426.3638-37.8292-55.556
364.0145-1.7524-1.96523.2816-2.21322.4927-0.01750.0602-0.0580.09450.1304-0.0781-0.1505-0.0136-0.1129-0.2723-0.0035-0.06610.21150.1305-0.015728.1704-30.1031-51.4109
370.37380.9742-1.25420.379-0.28740.2539-0.0007-0.03420.0189-0.0063-0.01070.05390.0433-0.02820.0114-0.1263-0.17030.02210.061-0.00810.0253-3.5544-84.759846.9882
381.8236-2.20431.0216-0.2013-0.18080.7670.01220.0272-0.05170.02030.0590.0055-0.0660.0558-0.0712-0.15530.01510.02830.2213-0.08340.0030.9768-88.984650.1395
390.1005-1.64622.46560.0781-0.60041.31950.00420.0331-0.07170.007-0.06110.00940.02550.11870.0568-0.21050.0269-0.01570.1108-0.03650.0832-3.3911-91.558146.9287
401.75321.999-2.5541-0.0292-0.39772.0387-0.03220.001-0.02860.08570.00260.00770.01530.00830.0296-0.2055-0.08570.08450.15550.07650.0715-11.9115-88.565251.8276
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1CHAIN A AND RESID 1 - 119
2X-RAY DIFFRACTION2CHAIN A AND RESID 120 - 191
3X-RAY DIFFRACTION3CHAIN A AND RESID 192 - 217
4X-RAY DIFFRACTION4CHAIN B AND RESID 1 - 137
5X-RAY DIFFRACTION5CHAIN B AND RESID 138 - 155
6X-RAY DIFFRACTION6CHAIN B AND RESID 156 - 212
7X-RAY DIFFRACTION7CHAIN C AND RESID 1 - 112
8X-RAY DIFFRACTION8CHAIN C AND RESID 113 - 211
9X-RAY DIFFRACTION9CHAIN C AND RESID 212 - 217
10X-RAY DIFFRACTION10CHAIN D AND RESID 1 - 18
11X-RAY DIFFRACTION11CHAIN D AND RESID 19 - 104
12X-RAY DIFFRACTION12CHAIN D AND RESID 105 - 212
13X-RAY DIFFRACTION13CHAIN E AND RESID 1 - 107
14X-RAY DIFFRACTION14CHAIN E AND RESID 108 - 190
15X-RAY DIFFRACTION15CHAIN E AND RESID 191 - 217
16X-RAY DIFFRACTION16CHAIN F AND RESID 1 - 104
17X-RAY DIFFRACTION17CHAIN F AND RESID 105 - 149
18X-RAY DIFFRACTION18CHAIN F AND RESID 150 - 212
19X-RAY DIFFRACTION19CHAIN H AND RESID 1 - 87
20X-RAY DIFFRACTION20CHAIN H AND RESID 88 - 119
21X-RAY DIFFRACTION21CHAIN H AND RESID 120 - 217
22X-RAY DIFFRACTION22CHAIN L AND RESID 1 - 141
23X-RAY DIFFRACTION23CHAIN L AND RESID 142 - 183
24X-RAY DIFFRACTION24CHAIN L AND RESID 184 - 212
25X-RAY DIFFRACTION25CHAIN Q AND RESID 300 - 319
26X-RAY DIFFRACTION26CHAIN Q AND RESID 320 - 341
27X-RAY DIFFRACTION27CHAIN Q AND RESID 342 - 355
28X-RAY DIFFRACTION28CHAIN Q AND RESID 356 - 393
29X-RAY DIFFRACTION29CHAIN R AND RESID 300 - 319
30X-RAY DIFFRACTION30CHAIN R AND RESID 320 - 342
31X-RAY DIFFRACTION31CHAIN R AND RESID 349 - 369
32X-RAY DIFFRACTION32CHAIN R AND RESID 370 - 395
33X-RAY DIFFRACTION33CHAIN S AND RESID 300 - 331
34X-RAY DIFFRACTION34CHAIN S AND RESID 332 - S|341
35X-RAY DIFFRACTION35CHAIN S AND RESID 342 - 358
36X-RAY DIFFRACTION36CHAIN S AND RESID 359 - 394
37X-RAY DIFFRACTION37CHAIN T AND RESID 300 - 319
38X-RAY DIFFRACTION38CHAIN T AND RESID 320 - 340
39X-RAY DIFFRACTION39CHAIN T AND RESID 341 - 369
40X-RAY DIFFRACTION40CHAIN T AND RESID 370 - 394

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