[English] 日本語
![](img/lk-miru.gif)
- PDB-4akr: Crystal Structure of the cytoplasmic actin capping protein Cap32_... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 4akr | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal Structure of the cytoplasmic actin capping protein Cap32_34 from Dictyostelium discoideum | ||||||
![]() |
| ||||||
![]() | ACTIN-BINDING PROTEIN | ||||||
Function / homology | ![]() Factors involved in megakaryocyte development and platelet production / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / COPI-mediated anterograde transport / F-actin capping protein complex / barbed-end actin filament capping / response to bacterium / cell morphogenesis / actin filament binding / actin cytoskeleton organization / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Eckert, C. / Goretzki, A. / Faberova, M. / Kollmar, M. | ||||||
![]() | ![]() Title: Conservation and Divergence between Cytoplasmic and Muscle-Specific Actin Capping Proteins: Insights from the Crystal Structure of Cytoplasmic CAP32/34 from Dictyostelium Discoideum. Authors: Eckert, C. / Goretzki, A. / Faberova, M. / Kollmar, M. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 213.9 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 171.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 452.5 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 466.3 KB | Display | |
Data in XML | ![]() | 40.3 KB | Display | |
Data in CIF | ![]() | 57 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1iznS S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 31194.803 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Description: CDNA FROM THE DICTYOSTELIUM CDNA PROJECT IN JAPAN AND THE JAPANESE NATIONAL BIORESOURCE PROJECT Production host: ![]() ![]() #2: Protein | Mass: 32873.859 Da / Num. of mol.: 2 / Fragment: RESIDUES 2-272 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Description: CDNA FROM THE DICTYOSTELIUM CDNA PROJECT IN JAPAN AND THE JAPANESE NATIONAL BIORESOURCE PROJECT Production host: ![]() ![]() #3: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 46 % / Description: NONE |
---|---|
Crystal grow | pH: 7.5 / Details: 100 MM HEPES PH 7.5, 17% (W/V) PEG 8000 |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: May 4, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8726 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. obs: 60185 / % possible obs: 99.8 % / Observed criterion σ(I): 3 / Redundancy: 6.1 % / Rmerge(I) obs: 0.15 / Net I/σ(I): 14.15 |
Reflection shell | Resolution: 2.2→2.3 Å / Redundancy: 6.1 % / Rmerge(I) obs: 0.84 / Mean I/σ(I) obs: 3.83 / % possible all: 99.6 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1IZN Resolution: 2.2→50 Å / Cross valid method: THROUGHOUT / σ(F): 3 / Stereochemistry target values: MAXIMUM LIKELIHOOD
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 15.4 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→50 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
|