Mass: 18960.732 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) BACILLUS SUBTILIS SUBSP. SUBTILIS (bacteria) Strain: 168 / Plasmid: PET2818 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): PREP4GROESL / References: UniProt: O34816
Compound details
HIS 126 IS PROTONATED ON THE ND1 (ND1=ATOM 1950 AND HD1=ATOM 1958) AND NON PROTONATED ON THE NE2 ...HIS 126 IS PROTONATED ON THE ND1 (ND1=ATOM 1950 AND HD1=ATOM 1958) AND NON PROTONATED ON THE NE2 (ATOM 1953). CYS 142 IS PROTONATED ON THE SG (SG=ATOM 2164 AND HG=ATOM 2169).
Sequence details
GENE SEQUENCING LED TO THE REPLACEMENT OF THE FIRST MET BY GRKL, AND TO THE ADDITION OF THE C- ...GENE SEQUENCING LED TO THE REPLACEMENT OF THE FIRST MET BY GRKL, AND TO THE ADDITION OF THE C-TERMINAL HIS-TAG GSHHHHHH
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Experimental details
-
Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
[1H
1
2
1
15N]-HSQC
1
3
1
13C]-NOESY-HSQ IN THE ALIPHATIC REGION
1
4
1
HN(CA)CB
1
5
1
HNCO
1
6
1
13C]-HSQC IN THE ALIPHATIC REGION
1
7
1
METHYLSELECTIVE [1H
1
8
1
13C] -HSQC-NOESY
1
9
1
13C]-HSQC IN THE AROMATIC REGION
1
10
1
15N] SOFAST-HMQC ON THE HISTIDINES
1
11
1
[1H-15N]-NOESY- HSQC
1
12
1
13C]- NOESY-HSQC IN THE AROMATIC REGION
NMR details
Text: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY AND N-H AND CA-HA RDCS ON 13C, 15N-LABELED LDTBS. THE 20 STRUCTURES OF MINIMAL ENERGY WERE FURTHER REFINED IN WATER.
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Sample preparation
Details
Contents: 90% H2O/10% D2O
Sample conditions
Ionic strength: 0.15 M / pH: 6.5 / Pressure: 1.0 atm / Temperature: 298.0 K
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