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- PDB-3zjy: Crystal Structure of Importin 13 - RanGTP - eIF1A complex -

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Basic information

Entry
Database: PDB / ID: 3zjy
TitleCrystal Structure of Importin 13 - RanGTP - eIF1A complex
Components
  • EUKARYOTIC TRANSLATION INITIATION FACTOR 1A, X-CHROMOSOMAL
  • GTP-BINDING NUCLEAR PROTEIN RAN
  • IMPORTIN-13
KeywordsTRANSLATION / ACTIVE TRANSPORT / CELL NUCLEUS / NUCLEOCYTOPLASMIC TRANSPORT / MULTIPROTEIN COMPLEX / RNA-BINDING PROTEIN / RAN GTP-BINDING PROTEIN
Function / homology
Function and homology information


pre-miRNA export from nucleus / RNA nuclear export complex / snRNA import into nucleus / multi-eIF complex / manchette / cellular response to mineralocorticoid stimulus / translation factor activity, RNA binding / eukaryotic 43S preinitiation complex / Regulation of cholesterol biosynthesis by SREBP (SREBF) / importin-alpha family protein binding ...pre-miRNA export from nucleus / RNA nuclear export complex / snRNA import into nucleus / multi-eIF complex / manchette / cellular response to mineralocorticoid stimulus / translation factor activity, RNA binding / eukaryotic 43S preinitiation complex / Regulation of cholesterol biosynthesis by SREBP (SREBF) / importin-alpha family protein binding / eukaryotic 48S preinitiation complex / Rev-mediated nuclear export of HIV RNA / Nuclear import of Rev protein / protein localization to nucleolus / NEP/NS2 Interacts with the Cellular Export Machinery / tRNA processing in the nucleus / Postmitotic nuclear pore complex (NPC) reformation / GTP metabolic process / protein-containing complex localization / MicroRNA (miRNA) biogenesis / DNA metabolic process / Formation of the ternary complex, and subsequently, the 43S complex / Ribosomal scanning and start codon recognition / Translation initiation complex formation / dynein intermediate chain binding / mitotic sister chromatid segregation / viral process / ribosomal large subunit export from nucleus / Formation of a pool of free 40S subunits / spermatid development / nuclear pore / GTP hydrolysis and joining of the 60S ribosomal subunit / sperm flagellum / L13a-mediated translational silencing of Ceruloplasmin expression / ribosomal subunit export from nucleus / ribosomal small subunit export from nucleus / translation initiation factor activity / centriole / protein export from nucleus / ribosome assembly / mitotic spindle organization / male germ cell nucleus / hippocampus development / translational initiation / Transcriptional regulation by small RNAs / recycling endosome / positive regulation of protein import into nucleus / small GTPase binding / protein import into nucleus / GDP binding / melanosome / nuclear envelope / mitotic cell cycle / actin cytoskeleton organization / G protein activity / midbody / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / tRNA binding / cadherin binding / protein heterodimerization activity / protein domain specific binding / cell division / GTPase activity / chromatin binding / synapse / chromatin / GTP binding / protein-containing complex binding / nucleolus / magnesium ion binding / protein-containing complex / RNA binding / extracellular exosome / nucleoplasm / membrane / nucleus / cytoplasm / cytosol
Similarity search - Function
Importin 13 repeat 2 / : / : / Importin 13 repeat / Importin 13 repeat / Importin 13, repeat 1 / : / Importin 13 repeat / Importin 13 repeat / Transportin-3/Importin-13 second TPR domain ...Importin 13 repeat 2 / : / : / Importin 13 repeat / Importin 13 repeat / Importin 13, repeat 1 / : / Importin 13 repeat / Importin 13 repeat / Transportin-3/Importin-13 second TPR domain / Transportin-3/Importin-13 third TPR domain / Transportin-3/Importin-13 fourth TPR domain / : / Translation initiation factor 1A (eIF-1A), conserved site / Eukaryotic initiation factor 1A signature. / eukaryotic translation initiation factor 1A / Translation initiation factor 1A (eIF-1A) / Exportin-1/Importin-beta-like / Exportin 1-like protein / Ran GTPase / Small GTPase Ran-type domain profile. / RNA-binding domain, S1, IF1 type / Translation initiation factor 1A / IF-1 / S1 domain IF1 type profile. / Importin-beta N-terminal domain / Importin-beta N-terminal domain / Importin-beta N-terminal domain profile. / Importin-beta, N-terminal domain / Ran (Ras-related nuclear proteins) /TC4 subfamily of small GTPases / Nucleic acid-binding proteins / Rho (Ras homology) subfamily of Ras-like small GTPases / Ras subfamily of RAS small GTPases / Small GTPase / Ras family / Rab subfamily of small GTPases / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Armadillo-like helical / Small GTP-binding protein domain / Armadillo-type fold / P-loop containing nucleotide triphosphate hydrolases / Nucleic acid-binding, OB-fold / Beta Barrel / Rossmann fold / P-loop containing nucleoside triphosphate hydrolase / 3-Layer(aba) Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
GUANOSINE-5'-TRIPHOSPHATE / Importin-13 / Eukaryotic translation initiation factor 1A, X-chromosomal / GTP-binding nuclear protein Ran
Similarity search - Component
Biological speciesHOMO SAPIENS (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.6 Å
AuthorsGruenwald, M. / Lazzaretti, D. / Bono, F.
CitationJournal: Embo J. / Year: 2013
Title: Structural Basis for the Nuclear Export Activity of Importin13.
Authors: Grunwald, M. / Lazzaretti, D. / Bono, F.
History
DepositionJan 21, 2013Deposition site: PDBE / Processing site: PDBE
Revision 1.0Apr 10, 2013Provider: repository / Type: Initial release
Revision 1.1Dec 20, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Other / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_sf / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id
Remark 700 SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "CA" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "CA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 5-STRANDED BARREL THIS IS REPRESENTED BY A 6-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: GTP-BINDING NUCLEAR PROTEIN RAN
B: IMPORTIN-13
C: EUKARYOTIC TRANSLATION INITIATION FACTOR 1A, X-CHROMOSOMAL
D: GTP-BINDING NUCLEAR PROTEIN RAN
E: IMPORTIN-13
F: GTP-BINDING NUCLEAR PROTEIN RAN
G: IMPORTIN-13
hetero molecules


Theoretical massNumber of molelcules
Total (without water)401,25213
Polymers399,6107
Non-polymers1,6426
Water00
1
A: GTP-BINDING NUCLEAR PROTEIN RAN
B: IMPORTIN-13
C: EUKARYOTIC TRANSLATION INITIATION FACTOR 1A, X-CHROMOSOMAL
hetero molecules


Theoretical massNumber of molelcules
Total (without water)142,3825
Polymers141,8353
Non-polymers5472
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3510 Å2
ΔGint-24.2 kcal/mol
Surface area48180 Å2
MethodPISA
2
D: GTP-BINDING NUCLEAR PROTEIN RAN
E: IMPORTIN-13
hetero molecules


Theoretical massNumber of molelcules
Total (without water)129,4354
Polymers128,8882
Non-polymers5472
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3050 Å2
ΔGint-26.5 kcal/mol
Surface area47780 Å2
MethodPISA
3
F: GTP-BINDING NUCLEAR PROTEIN RAN
G: IMPORTIN-13
hetero molecules


Theoretical massNumber of molelcules
Total (without water)129,4354
Polymers128,8882
Non-polymers5472
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3610 Å2
ΔGint-24.9 kcal/mol
Surface area47720 Å2
MethodPISA
Unit cell
Length a, b, c (Å)186.500, 100.400, 274.720
Angle α, β, γ (deg.)90.00, 90.91, 90.00
Int Tables number5
Space group name H-MC121

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Components

#1: Protein GTP-BINDING NUCLEAR PROTEIN RAN / ANDROGEN RECEPTOR-ASSOCIATED PROTEIN 24 / GTPASE RAN / RAS-LIKE PROTEIN TC4 / RAS-RELATED NUCLEAR PROTEIN


Mass: 20593.967 Da / Num. of mol.: 3 / Fragment: RESIDUES 1-180 / Mutation: YES
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P62826
#2: Protein IMPORTIN-13 / IMP13 / KARYOPHERIN-13 / KAP13 / RAN-BINDING PROTEIN 13 / RANBP13 / IMPORTIN13


Mass: 108293.719 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: O94829
#3: Protein EUKARYOTIC TRANSLATION INITIATION FACTOR 1A, X-CHROMOSOMAL / EIF-1A X ISOFORM / EUKARYOTIC TRANSLATION INITIATION FACTOR 4C / EIF-4C / TRANSLATION INITIATION FACTOR 1A


Mass: 12946.941 Da / Num. of mol.: 1 / Fragment: RESIDUES 1-112
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P47813
#4: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#5: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Mg

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.1 Å3/Da / Density % sol: 60 % / Description: NONE

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 0.97919
DetectorType: DECTRIS PILATUS / Detector: PIXEL / Date: Nov 28, 2012
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97919 Å / Relative weight: 1
ReflectionResolution: 3.6→50 Å / Num. obs: 57266 / % possible obs: 96.6 % / Observed criterion σ(I): 2 / Redundancy: 3.4 % / Biso Wilson estimate: 116.11 Å2 / Rmerge(I) obs: 0.08 / Net I/σ(I): 11.35
Reflection shellResolution: 3.6→3.7 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.92 / Mean I/σ(I) obs: 2.08 / % possible all: 87.4

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Processing

Software
NameVersionClassification
PHENIX(PHENIX.REFINE)refinement
XDSdata reduction
XSCALEdata scaling
PHASERMRphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 2X19

2x19


Resolution: 3.6→49.549 Å / SU ML: 0.61 / σ(F): 1.99 / Phase error: 33.61 / Stereochemistry target values: MLHL
RfactorNum. reflection% reflection
Rfree0.2879 2843 5 %
Rwork0.2563 --
obs0.2579 57163 96.49 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 93.34 Å2
Refinement stepCycle: LAST / Resolution: 3.6→49.549 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms21609 0 99 0 21708
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00322348
X-RAY DIFFRACTIONf_angle_d0.76430839
X-RAY DIFFRACTIONf_dihedral_angle_d14.3736865
X-RAY DIFFRACTIONf_chiral_restr0.0493738
X-RAY DIFFRACTIONf_plane_restr0.0034075
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.6-3.66210.42461230.39342535X-RAY DIFFRACTION91
3.6621-3.72860.46471210.41052424X-RAY DIFFRACTION88
3.7286-3.80030.32251540.32812758X-RAY DIFFRACTION99
3.8003-3.87790.36381400.33722649X-RAY DIFFRACTION95
3.8779-3.96210.41621200.36332240X-RAY DIFFRACTION81
3.9621-4.05430.34271380.30172805X-RAY DIFFRACTION99
4.0543-4.15560.31551430.28542714X-RAY DIFFRACTION99
4.1556-4.26790.36011440.26842761X-RAY DIFFRACTION99
4.2679-4.39340.28351510.25892778X-RAY DIFFRACTION99
4.3934-4.53510.26011430.23742785X-RAY DIFFRACTION99
4.5351-4.69710.28331410.24082733X-RAY DIFFRACTION99
4.6971-4.8850.28731470.23372786X-RAY DIFFRACTION99
4.885-5.10710.28881460.24072745X-RAY DIFFRACTION99
5.1071-5.37610.28611470.25692793X-RAY DIFFRACTION99
5.3761-5.71250.37591420.27672788X-RAY DIFFRACTION99
5.7125-6.15280.33661510.28142795X-RAY DIFFRACTION99
6.1528-6.77060.29761450.27092792X-RAY DIFFRACTION99
6.7706-7.74710.2811500.24512801X-RAY DIFFRACTION98
7.7471-9.74830.16551470.18072811X-RAY DIFFRACTION98
9.7483-49.55340.25931500.22822827X-RAY DIFFRACTION96
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.1076-0.0473-0.10060.09770.11970.16340.03650.05170.05690.00610.01770.01890.01490.0220.08660.2538-0.0021-0.19460.1527-0.10350.254556.3752-4.5344-4.2503
20.00350.0011-0.00840.00190.00190.0498-0.0741-0.00710.0320.0191-0.05570.0263-0.00350.00420.00030.4167-0.08570.04980.1069-0.00580.394461.60175.58824.2086
30.09390.01740.05230.2591-0.18970.1909-0.1054-0.01870.03060.0773-0.1077-0.0616-0.1350.0662-0.18520.2839-0.03920.10250.1140.0138-0.01662.93677.74220.0733
40.0380.02840.00410.02330.0004-0.0022-0.0039-0.0066-0.0729-0.00920.0277-0.0445-0.0563-0.01740.10960.13480.11810.21680.21810.20.329761.7617-6.7193-4.2765
50.0228-0.010.00910.01130.00430.0195-0.0219-0.01510.03780.0104-0.0237-0.0074-0.034-0.0052-0.02040.8993-0.0521-0.2250.56750.10760.679849.267614.44181.1451
60.00180.0009-0.00140.00350.00040.0054-0.0175-0.00170.04290.0132-0.0166-0.0019-0.0471-0.0275-0.00020.98980.13930.05190.6048-0.03940.71146.49081.4183-5.1426
70.01340.00130.01110.10740.05160.0580.0905-0.0776-0.0207-0.0833-0.08430.16560.0214-0.1087-0.03860.5363-0.0089-0.10910.265-0.0310.228751.45220.899311.7773
80.000600.00060-0-0.0006-0.02250.001-0.01820.001-0.00340.10250.0423-0.09950.00020.9209-0.08230.14010.69720.26060.698352.3141-8.311313.0151
90.1999-0.0530.03480.0184-0.02820.087-0.116-0.0276-0.0151-0.084-0.0354-0.09190.11630.0403-0.13460.32740.1275-0.14330.05530.06270.417963.1275-9.39355.9079
100.2806-0.0421-0.21870.1855-0.03510.44130.2051-0.09450.3435-0.1530.085-0.1985-0.1934-0.15560.59520.3379-0.0003-0.0089-0.10940.0258-0.023731.05080.872.1475
110.2093-0.02390.10130.40350.26780.5319-0.0661-0.1503-0.06170.06090.2637-0.18260.026-0.10030.4030.7139-0.03980.01370.0917-0.1401-0.071850.1016-18.819232.4422
120.12-0.02120.05860.1579-0.12920.0221-0.25970.21430.08010.28410.1225-0.2844-0.03570.185-0.51970.0715-0.4191-0.3110.2833-0.0680.026883.11041.978729.3154
130.0385-0.0390.00110.15260.07590.0773-0.101-0.0392-0.02440.24320.10220.0619-0.1819-0.15170.04130.7579-0.1481-0.2367-0.0061-0.1230.097556.66330.367521.9894
140.0837-0.02440.00840.00790.00090.00260.0001-0.0251-0.02080.0606-0.00370.0163-0.0030.002-0.01050.42820.03480.13170.4585-0.03690.613664.6914-4.256891.4202
150.0146-0.0038-0.00930.01010.00780.0151-0.03220.0813-0.0325-0.0590.0015-0.1507-0.0777-0.0581-00.5901-0.01220.02450.63660.04940.867753.70471.673692.4836
160.0387-0.0271-0.0790.01810.05530.1888-0.18360.00810.19670.0908-0.0067-0.1463-0.07150.16440.0620.5575-0.167-0.25250.56950.13480.85667.7565.672384.6109
170.01020.03570.01460.11890.04730.02030.0174-0.0375-0.06230.0121-0.02940.05080.0353-0.0261-0.06590.3515-0.108-0.31910.4446-0.04530.384955.2292-14.119192.0939
180.0043-0.02590.01780.2008-0.17460.1562-0.2263-0.00430.0256-0.0552-0.14530.0213-0.0221-0.1154-0.35360.1927-0.0211-0.05040.3377-0.14960.406566.4342-10.222196.5717
190.01730.0283-0.01470.0339-0.0210.0226-0.0880.0205-0.0272-0.1914-0.0039-0.0450.1184-0.0638-0.02740.58320.055-0.01990.3082-0.17970.592266.1815-2.4212101.8762
200.00110.0011-0.01290.0033-0.01850.09990.04510.0069-0.04590.10780.1142-0.12110.12360.2568-0.09560.7628-0.0201-0.37370.4401-0.21790.524868.12612.3973103.8155
210.07610.02220.00980.0562-0.03570.03020.0388-0.00880.0356-0.03470.1006-0.0136-0.03060.06020.05040.4027-0.2636-0.15840.5682-0.07151.184976.3697.370792.9672
220.57720.18380.17970.18540.10190.50010.20030.5913-0.07770.02680.0913-0.13890.260.0614-0.08570.1161-0.01570.06060.2104-0.08890.275971.265-27.230290.5963
230.147-0.06450.04550.13560.11990.2266-0.2240.03030.1784-0.0085-0.06130.06750.0304-0.2793-0.3974-0.2446-0.07180.26870.38520.0473-0.12785.2183-4.4554120.7337
240.26020.06830.30080.07180.23030.3874-0.3465-0.38610.4652-0.2632-0.23580.3216-0.3727-0.7249-0.3412-0.54640.3217-0.08310.4423-0.27040.709749.623617.1068115.4894
250.0469-0.00230.00330.1170.0140.14050.1588-0.1265-0.08720.1508-0.0681-0.1550.046-0.03740.10480.9908-0.344-0.11790.76260.35931.039335.9295-22.0535110.6972
260.18130.12840.13310.09980.07380.1576-0.0288-0.01180.00010.049-0.0287-0.0005-0.01130.0287-0.0355-0.08610.0105-0.03210.01020.11280.243928.1078-45.227991.9537
270.01810.00220.00950.0065-0.00750.0168-0.0328-0.0021-0.02690.0257-0.08170.01880.0473-0.0031-0.02160.39030.14520.42980.46020.04350.843426.4881-55.896391.8362
280.1827-0.05480.14070.0830.08660.3339-0.1117-0.0535-0.12390.106-0.04870.08090.0808-0.2834-0.1980.3101-0.12630.30370.51140.25320.605524.9465-41.906288.8329
290.006-0.0037-0.00510.00270.00480.0089-0.0036-0.00570.0082-0.01420.0050.0055-0.0140.01470.00111.2276-0.1515-0.11351.1933-0.16931.367327.1505-54.37471.0373
300.05210.0067-0.02990.01110.00070.0123-0.01550.1022-0.0466-0.05850.0233-0.04730.02110.01280.06390.2866-0.16170.49190.50310.09870.798237.1343-43.776482.6686
310.15760.1439-0.08180.264-0.11350.0501-0.07330.0222-0.3623-0.4155-0.01950.14960.2465-0.0439-0.34560.0827-0.2584-0.00680.64260.1850.440312.1907-26.845391.5834
320.293-0.1738-0.22010.19430.05070.41750.19050.2776-0.0136-0.11270.02260.05040.0539-0.26040.68830.2727-0.1688-0.00090.3153-0.037-0.390341.2769-31.395360.0836
330.00840.0016-0.0039-0.00010.00260.0790.04680.1032-0.3420.0350.01450.14780.26750.05810.11520.35320.32320.1576-0.4503-0.961-0.107249.368-69.183663.1612
340.0483-0.0308-0.03440.2107-0.04430.15120.0336-0.0589-0.0150.00470.03670.31820.4032-0.27790.17240.3446-0.37480.3977-0.1888-0.25970.870815.106-74.169272.0207
350.0151-0.0029-0.00740.0141-0.00040.00660.01550.1307-0.0001-0.06340.01670.03590.0075-0.0319-01.3806-0.1641-0.29981.48180.06731.4960.5252-11.4201131.714
360.0117-0.00330.00550.0029-0.00510.00690.02390.01470.02690.01510.01880.0505-0.041-0.034901.2804-0.1518-0.26051.5415-0.1461.420962.2348-5.562131.4002
370.00290.0157-0.00430.1415-0.03690.0107-0.00680.00260.01950.0002-0.0068-0.0058-0.0090.00880.00571.0804-0.11580.03141.06180.02951.150157.3287-6.8506139.7279
380.00250.00150.00280.0010.00020.0049-0.03360.01130.0368-0.0244-0.0812-0.0121-0.02390.0339-0.00010.889-0.20630.1121.0024-0.19290.887962.6015-10.1989125.1195
390.0050.00630.00350.01360.00690.007-0.0228-0.01210.01820.0227-0.0165-0.0165-0.02620.006-01.1901-0.19420.14051.2147-0.01541.228843.8374-12.7514131.0211
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1CHAIN F AND (RESID 8 THROUGH 17 )
2X-RAY DIFFRACTION2CHAIN F AND (RESID 18 THROUGH 32 )
3X-RAY DIFFRACTION3CHAIN F AND (RESID 33 THROUGH 53 )
4X-RAY DIFFRACTION4CHAIN F AND (RESID 54 THROUGH 66 )
5X-RAY DIFFRACTION5CHAIN F AND (RESID 67 THROUGH 75 )
6X-RAY DIFFRACTION6CHAIN F AND (RESID 76 THROUGH 84 )
7X-RAY DIFFRACTION7CHAIN F AND (RESID 85 THROUGH 137 )
8X-RAY DIFFRACTION8CHAIN F AND (RESID 138 THROUGH 149 )
9X-RAY DIFFRACTION9CHAIN F AND (RESID 150 THROUGH 177 )
10X-RAY DIFFRACTION10CHAIN G AND (RESID 19 THROUGH 301 )
11X-RAY DIFFRACTION11CHAIN G AND (RESID 302 THROUGH 476 )
12X-RAY DIFFRACTION12CHAIN G AND (RESID 477 THROUGH 781 )
13X-RAY DIFFRACTION13CHAIN G AND (RESID 782 THROUGH 953 )
14X-RAY DIFFRACTION14CHAIN A AND (RESID 9 THROUGH 22 )
15X-RAY DIFFRACTION15CHAIN A AND (RESID 23 THROUGH 45 )
16X-RAY DIFFRACTION16CHAIN A AND (RESID 46 THROUGH 65 )
17X-RAY DIFFRACTION17CHAIN A AND (RESID 66 THROUGH 75 )
18X-RAY DIFFRACTION18CHAIN A AND (RESID 76 THROUGH 111 )
19X-RAY DIFFRACTION19CHAIN A AND (RESID 112 THROUGH 132 )
20X-RAY DIFFRACTION20CHAIN A AND (RESID 133 THROUGH 158 )
21X-RAY DIFFRACTION21CHAIN A AND (RESID 159 THROUGH 177 )
22X-RAY DIFFRACTION22CHAIN B AND (RESID 17 THROUGH 251 )
23X-RAY DIFFRACTION23CHAIN B AND (RESID 252 THROUGH 454 )
24X-RAY DIFFRACTION24CHAIN B AND (RESID 455 THROUGH 874 )
25X-RAY DIFFRACTION25CHAIN B AND (RESID 875 THROUGH 952 )
26X-RAY DIFFRACTION26CHAIN D AND (RESID 8 THROUGH 22 )
27X-RAY DIFFRACTION27CHAIN D AND (RESID 23 THROUGH 53 )
28X-RAY DIFFRACTION28CHAIN D AND (RESID 54 THROUGH 122 )
29X-RAY DIFFRACTION29CHAIN D AND (RESID 123 THROUGH 132 )
30X-RAY DIFFRACTION30CHAIN D AND (RESID 133 THROUGH 177 )
31X-RAY DIFFRACTION31CHAIN E AND (RESID 17 THROUGH 267 )
32X-RAY DIFFRACTION32CHAIN E AND (RESID 268 THROUGH 502 )
33X-RAY DIFFRACTION33CHAIN E AND (RESID 503 THROUGH 700 )
34X-RAY DIFFRACTION34CHAIN E AND (RESID 701 THROUGH 951 )
35X-RAY DIFFRACTION35CHAIN C AND (RESID 28 THROUGH 51 )
36X-RAY DIFFRACTION36CHAIN C AND (RESID 52 THROUGH 68 )
37X-RAY DIFFRACTION37CHAIN C AND (RESID 69 THROUGH 75 )
38X-RAY DIFFRACTION38CHAIN C AND (RESID 76 THROUGH 94 )
39X-RAY DIFFRACTION39CHAIN C AND (RESID 95 THROUGH 108 )

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