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- PDB-4ydu: Crystal structure of E. coli YgjD-YeaZ heterodimer in complex with ADP -
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Open data
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Basic information
Entry | Database: PDB / ID: 4ydu | |||||||||
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Title | Crystal structure of E. coli YgjD-YeaZ heterodimer in complex with ADP | |||||||||
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![]() | TRANSFERASE / YgjD-YeaZ complex / t6A transferase | |||||||||
Function / homology | ![]() glycosylation-dependent protein binding / N6-L-threonylcarbamoyladenine synthase / N(6)-L-threonylcarbamoyladenine synthase activity / EKC/KEOPS complex / tRNA threonylcarbamoyladenosine modification / maintenance of translational fidelity / metallopeptidase activity / iron ion binding / magnesium ion binding / identical protein binding / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Zhang, W. / Collinet, B. / Perrochia, L. / Durand, D. / Van Tilbeurgh, H. | |||||||||
![]() | ![]() Title: The ATP-mediated formation of the YgjD-YeaZ-YjeE complex is required for the biosynthesis of tRNA t6A in Escherichia coli. Authors: Zhang, W. / Collinet, B. / Perrochia, L. / Durand, D. / Van Tilbeurgh, H. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 440.8 KB | Display | ![]() |
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PDB format | ![]() | 360.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.7 MB | Display | ![]() |
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Full document | ![]() | 1.7 MB | Display | |
Data in XML | ![]() | 46.8 KB | Display | |
Data in CIF | ![]() | 66.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4wq4C ![]() 4wq5C ![]() 2ivpS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 1 / Beg auth comp-ID: ARG / Beg label comp-ID: ARG / Refine code: 4
NCS ensembles :
NCS oper:
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Components
-Protein , 2 types, 4 molecules ABCD
#1: Protein | Mass: 36879.238 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 26033.635 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() References: UniProt: P76256 |
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-Non-polymers , 5 types, 459 molecules ![](data/chem/img/ADP.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/FE.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/FE.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-ADP / #4: Chemical | #5: Chemical | #6: Chemical | ChemComp-ACT / #7: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.46 % / Description: thin plate |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.4 Details: 30% PEG4000, 0.3M Lithium Sulfate, 0.1 M Sodium Acetate and 0.1 M HEPES PH range: 7.2 - 7.6 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Oct 2, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9801 Å / Relative weight: 1 |
Reflection | Resolution: 2.25→49.38 Å / Num. obs: 55653 / % possible obs: 97.9 % / Redundancy: 3.97 % / Rsym value: 0.121 / Net I/σ(I): 11 |
Reflection shell | Resolution: 2.25→2.33 Å / Rmerge(I) obs: 0.57 / Mean I/σ(I) obs: 2.46 / Rsym value: 0.483 / % possible all: 95.69 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2IVP Resolution: 2.33→49.38 Å / Cor.coef. Fo:Fc: 0.96 / Cor.coef. Fo:Fc free: 0.935 / SU B: 12.862 / SU ML: 0.171 / Cross valid method: THROUGHOUT / ESU R: 0.281 / ESU R Free: 0.209 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 35.999 Å2
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Refinement step | Cycle: 1 / Resolution: 2.33→49.38 Å
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Refine LS restraints |
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