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- PDB-3vun: Crystal structure of a influenza A virus (A/Aichi/2/1968 H3N2) he... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3vun | |||||||||
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Title | Crystal structure of a influenza A virus (A/Aichi/2/1968 H3N2) hemagglutinin in C2 space group. | |||||||||
![]() | (Hemagglutinin ...) x 2 | |||||||||
![]() | VIRAL PROTEIN / VIRAL ENVELOPE PROTEIN / MEMBRANE FUSION / Glycosilation | |||||||||
Function / homology | ![]() viral budding from plasma membrane / clathrin-dependent endocytosis of virus by host cell / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane / membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Yasutake, Y. / Suzuki, T. / Kawaguchi, A. / Nobusawa, E. | |||||||||
![]() | ![]() Title: Crystal structure of a influenza A virus (A/Aichi/2/1968 H3N2) hemagglutinin in C2 space group Authors: Yasutake, Y. / Suzuki, T. / Kawaguchi, A. / Nobusawa, E. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 592.9 KB | Display | ![]() |
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PDB format | ![]() | 497.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 3 MB | Display | ![]() |
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Full document | ![]() | 3 MB | Display | |
Data in XML | ![]() | 56 KB | Display | |
Data in CIF | ![]() | 73.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3hmgS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 1 / Refine code: 5
NCS ensembles :
NCS oper:
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Components
-Hemagglutinin ... , 2 types, 6 molecules ACEBDF
#1: Protein | Mass: 36238.652 Da / Num. of mol.: 3 / Mutation: G144S, I182V / Source method: isolated from a natural source Details: Mutations (G144S and I182V) occurred during laboratory passage Source: (natural) ![]() ![]() #2: Protein | Mass: 20198.324 Da / Num. of mol.: 3 / Mutation: E132D / Source method: isolated from a natural source Details: Mutations (E132D) occurred during laboratory passage Source: (natural) ![]() ![]() |
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-Sugars , 4 types, 15 molecules ![](data/chem/img/NAG.gif)
#3: Polysaccharide | Source method: isolated from a genetically manipulated source #4: Polysaccharide | Source method: isolated from a genetically manipulated source #5: Polysaccharide | Source method: isolated from a genetically manipulated source #6: Sugar | ChemComp-NAG / |
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-Details
Sequence details | THE SEQUENCE CONFLICTS IN THIS STRUCTURE WERE NATURALLY OCCURRENCE |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.28 Å3/Da / Density % sol: 62.5 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.25 Details: 0.2M imidazole (pH 7.0), 27% Jeffamine ED-2001 (pH 7.0), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: Jun 6, 2012 |
Radiation | Monochromator: Si(111) double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3→50 Å / Num. all: 43601 / Num. obs: 43601 / % possible obs: 99.7 % / Redundancy: 3.7 % / Rmerge(I) obs: 0.1 / Net I/σ(I): 21.4 |
Reflection shell | Resolution: 3→3.05 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.588 / Mean I/σ(I) obs: 2.6 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB code 3HMG Resolution: 3→30.59 Å / Cor.coef. Fo:Fc: 0.937 / Cor.coef. Fo:Fc free: 0.9 / SU B: 39.396 / SU ML: 0.336 / Cross valid method: THROUGHOUT / ESU R Free: 0.459 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 84.656 Å2
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Refinement step | Cycle: LAST / Resolution: 3→30.59 Å
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Refine LS restraints |
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Refine LS restraints NCS | Refine-ID: X-RAY DIFFRACTION
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LS refinement shell | Resolution: 3.003→3.081 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Origin x: 28.3353 Å / Origin y: 13.3963 Å / Origin z: 13.1834 Å
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Refinement TLS group |
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