THE CONSTRUCT (RESIDUES 82-244) WAS EXPRESSED WITH AN N-TERMINAL EXPRESSION / PURIFICATION TAG ...THE CONSTRUCT (RESIDUES 82-244) WAS EXPRESSED WITH AN N-TERMINAL EXPRESSION / PURIFICATION TAG MGSDKIHHHHHHAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVA PTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERV KGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYR EPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAK SLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGEPTTHENLYFQG. THE TAG WAS REMOVED WITH TEV PROTEASE LEAVING ONLY A GLYCINE (0) FOLLOWED BY THE TARGET SEQUENCE. WHILE THE CLONED SEQUENCE MATCHES ISOFORM 2 (ALSO KNOWN AS T) FOR UNIPROTKB ID P39688, RESIDUE NUMBERING IS BASED ON THE UNIPROT "CANONICAL" ISOFORM 1 (ALSO KNOWN AS B).
モノクロメーター: Double Crystal Si(111) / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.9793 Å / 相対比: 1
反射
解像度: 1.98→43.876 Å / Num. obs: 14960 / % possible obs: 97.8 % / Observed criterion σ(I): -3 / Biso Wilson estimate: 32.893 Å2 / Rmerge(I) obs: 0.058 / Net I/σ(I): 15.73
反射 シェル
Diffraction-ID: 1
解像度 (Å)
最高解像度 (Å)
Rmerge(I) obs
Mean I/σ(I) obs
Num. measured obs
Num. unique obs
% possible all
1.98-2.05
0.811
1.8
6306
1420
96.9
2.05-2.13
0.535
3.1
6356
1411
95.1
2.13-2.23
0.402
3.8
7506
1545
98.9
2.23-2.35
0.32
5.2
6548
1439
93.4
2.35-2.49
0.208
7
7045
1435
99.9
2.49-2.69
0.144
10.1
7381
1551
98
2.69-2.96
0.081
16.1
7421
1521
99.7
2.96-3.38
0.042
27.2
7152
1495
99.6
3.38-4.25
0.033
37.1
6611
1477
96.9
4.25
0.028
42.5
7413
1616
99.5
-
位相決定
位相決定
手法: 分子置換
-
解析
ソフトウェア
名称
バージョン
分類
NB
MolProbity
3beta29
モデル構築
PDB_EXTRACT
3.1
データ抽出
MOLREP
位相決定
XSCALE
December6, 2010
データスケーリング
BUSTER-TNT
2.10.0
精密化
XDS
データ削減
BUSTER
2.10.0
精密化
精密化
構造決定の手法: 分子置換 / 解像度: 1.98→43.876 Å / Cor.coef. Fo:Fc: 0.9501 / Cor.coef. Fo:Fc free: 0.9344 / Occupancy max: 1 / Occupancy min: 0.4 / 交差検証法: THROUGHOUT / σ(F): 0 詳細: 1. A MET-INHIBITION PROTOCOL WAS USED FOR SELENOMETHIONINE INCORPORATION DURING PROTEIN EXPRESSION. HOWEVER, THE SE-MET SIDE-CHAIN IS DISORDERED. 2. ATOM RECORD CONTAINS SUM OF TLS AND ...詳細: 1. A MET-INHIBITION PROTOCOL WAS USED FOR SELENOMETHIONINE INCORPORATION DURING PROTEIN EXPRESSION. HOWEVER, THE SE-MET SIDE-CHAIN IS DISORDERED. 2. ATOM RECORD CONTAINS SUM OF TLS AND RESIDUAL B FACTORS. ANISOU RECORD CONTAINS SUM OF TLS AND RESIDUAL U FACTORS. 3. GLYCEROL, SODIUM AND CHLORIDE MODELED IS PRESENT IN CRYO/CRYSTALLIZATION CONDITIONS.