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基本情報
登録情報 | データベース: PDB / ID: 3ual | ||||||
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タイトル | Crystal Structure of 14-3-3 epsilon with Mlf1 peptide | ||||||
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![]() | SIGNALING PROTEIN/PROTEIN BINDING / Adapter protein / ALL HELICAL / PHOSPHOPETIDE / SIGNALING PROTEIN-PROTEIN BINDING complex | ||||||
機能・相同性 | ![]() : / regulation of heart rate by hormone / regulation of potassium ion transmembrane transporter activity / negative regulation of calcium ion transmembrane transporter activity / membrane repolarization during cardiac muscle cell action potential / negative regulation of toll-like receptor signaling pathway / regulation of membrane repolarization / protein localization to endoplasmic reticulum / regulation of cell cycle G1/S phase transition / NADE modulates death signalling ...: / regulation of heart rate by hormone / regulation of potassium ion transmembrane transporter activity / negative regulation of calcium ion transmembrane transporter activity / membrane repolarization during cardiac muscle cell action potential / negative regulation of toll-like receptor signaling pathway / regulation of membrane repolarization / protein localization to endoplasmic reticulum / regulation of cell cycle G1/S phase transition / NADE modulates death signalling / myeloid progenitor cell differentiation / RAB GEFs exchange GTP for GDP on RABs / Signaling by Hippo / negative regulation of calcium ion export across plasma membrane / cytoplasmic pattern recognition receptor signaling pathway / Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models / regulation of heart rate by cardiac conduction / protein localization to nucleus / phosphoserine residue binding / calcium channel regulator activity / Regulation of HSF1-mediated heat shock response / HSF1 activation / Activation of BAD and translocation to mitochondria / potassium channel regulator activity / protein targeting / SARS-CoV-2 targets host intracellular signalling and regulatory pathways / Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex / signaling adaptor activity / SARS-CoV-1 targets host intracellular signalling and regulatory pathways / RHO GTPases activate PKNs / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of mitotic centrosome proteins and complexes / regulation of cytosolic calcium ion concentration / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / protein sequestering activity / regulation of mitotic cell cycle / substantia nigra development / AURKA Activation by TPX2 / ciliary basal body / positive regulation of protein export from nucleus / regulation of signal transduction by p53 class mediator / Translocation of SLC2A4 (GLUT4) to the plasma membrane / mitochondrial membrane / hippocampus development / TP53 Regulates Metabolic Genes / phosphoprotein binding / neuron migration / cell cycle / cilium / cerebral cortex development / histone deacetylase binding / Regulation of PLK1 Activity at G2/M Transition / MAPK cascade / melanosome / MHC class II protein complex binding / cellular response to heat / scaffold protein binding / protein phosphatase binding / transmembrane transporter binding / intracellular signal transduction / cadherin binding / protein heterodimerization activity / protein domain specific binding / focal adhesion / DNA-templated transcription / ubiquitin protein ligase binding / regulation of DNA-templated transcription / perinuclear region of cytoplasm / enzyme binding / signal transduction / endoplasmic reticulum / DNA binding / RNA binding / extracellular exosome / identical protein binding / membrane / nucleus / cytosol / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Weyand, M. / Ottmann, C. | ||||||
![]() | ![]() タイトル: Structural insights of the MLF1/14-3-3 interaction. 著者: Molzan, M. / Weyand, M. / Rose, R. / Ottmann, C. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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PDBx/mmCIF形式 | ![]() | 117.9 KB | 表示 | ![]() |
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PDB形式 | ![]() | 92.1 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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Components on special symmetry positions |
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要素
#1: タンパク質 | 分子量: 26617.340 Da / 分子数: 1 / 断片: UNP residues 1-232 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() | ||
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#2: タンパク質・ペプチド | 分子量: 1749.922 Da / 分子数: 1 / 断片: UNP residues 29-42 / 由来タイプ: 合成 / 詳細: This sequence occurs naturally in humans. / 由来: (合成) ![]() | ||
#3: 化合物 | #4: 水 | ChemComp-HOH / | |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.32 Å3/Da / 溶媒含有率: 46.95 % |
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結晶化 | 温度: 277 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 5.6 詳細: 0.1 M Na-Citrate, 35% tert-butanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: MARMOSAIC 225 mm CCD / 検出器: CCD / 日付: 2008年1月25日 |
放射 | モノクロメーター: LN2 cooled fixed-exit Si(111) monochromator プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.97906 Å / 相対比: 1 |
反射 | 解像度: 1.8→100 Å / Num. all: 24815 / Num. obs: 24289 / % possible obs: 97.9 % / Observed criterion σ(I): -3 / 冗長度: 6.1 % / Biso Wilson estimate: 32.9 Å2 / Rmerge(I) obs: 0.068 / Rsym value: 0.061 / Net I/σ(I): 16.8 |
反射 シェル | 解像度: 1.8→1.9 Å / 冗長度: 6.2 % / Rmerge(I) obs: 0.456 / Mean I/σ(I) obs: 4.1 / Num. unique all: 3568 / Rsym value: 0.45 / % possible all: 97.6 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: PDB ENTRY 2BR9 解像度: 1.8→35.27 Å / Cor.coef. Fo:Fc: 0.964 / Cor.coef. Fo:Fc free: 0.937 / SU B: 4.996 / SU ML: 0.077 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R Free: 0.125 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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溶媒の処理 | イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 32.07 Å2
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精密化ステップ | サイクル: LAST / 解像度: 1.8→35.27 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 1.8→1.847 Å / Total num. of bins used: 20
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精密化 TLS | 手法: refined / Origin x: -18.8512 Å / Origin y: 5.6095 Å / Origin z: 20.9416 Å
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