Crystal structure of Clostridium acetobutylicum aspartate kinase (CaAK): An important allosteric enzyme for industrial amino acids production
要素
Aspartokinase
キーワード
TRANSFERASE / Structural Genomics / ACT domains / Regulatory domains / Kinase / PSI-2 / Protein Structure Initiative / New York SGX Research Center for Structural Genomics / NYSGXRC
機能・相同性
機能・相同性情報
aspartate kinase / aspartate kinase activity / threonine biosynthetic process / lysine biosynthetic process via diaminopimelate / ATP binding 類似検索 - 分子機能
構造決定の手法: 単波長異常分散 / 解像度: 3→44.16 Å / Cor.coef. Fo:Fc: 0.945 / Cor.coef. Fo:Fc free: 0.896 / SU B: 50.934 / SU ML: 0.423 / Isotropic thermal model: Isotropic / 交差検証法: THROUGHOUT / ESU R Free: 0.5 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. THE FRIEDEL PAIRS WERE USED FOR PHASING.
Rfactor
反射数
%反射
Selection details
Rfree
0.27329
924
80 %
RANDOM
Rwork
0.20622
-
-
-
obs
0.20678
111696
92.12 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK