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- PDB-3srn: STRUCTURAL CHANGES THAT ACCOMPANY THE REDUCED CATALYTIC EFFICIENC... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3srn | ||||||
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Title | STRUCTURAL CHANGES THAT ACCOMPANY THE REDUCED CATALYTIC EFFICIENCY OF TWO SEMISYNTHETIC RIBONUCLEASE ANALOGS | ||||||
![]() | (RIBONUCLEASE A) x 2 | ||||||
![]() | HYDROLASE(NUCLEIC ACID / RNA) | ||||||
Function / homology | ![]() pancreatic ribonuclease / ribonuclease A activity / RNA nuclease activity / nucleic acid binding / lyase activity / defense response to Gram-positive bacterium / extracellular region Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | deMel, V.S.J. / Martin, P.D. / Doscher, M.S. / Edwards, B.F.P. | ||||||
![]() | ![]() Title: Structural changes that accompany the reduced catalytic efficiency of two semisynthetic ribonuclease analogs. Authors: deMel, V.S. / Martin, P.D. / Doscher, M.S. / Edwards, B.F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 36.1 KB | Display | ![]() |
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PDB format | ![]() | 27.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO A 93 |
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Components
#1: Protein | Mass: 12494.965 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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#2: Protein/peptide | Mass: 1230.390 Da / Num. of mol.: 1 / Source method: obtained synthetically / References: UniProt: P61823 |
#3: Chemical | ChemComp-SO4 / |
#4: Water | ChemComp-HOH / |
Compound details | FEATURES OF THE STRUCTURE: 1. IN SPITE OF AN EIGHT-RESIDUE REDUNDANCY BETWEEN RNASE 1 - 118 AND ...FEATURES OF THE STRUCTURE: 1. IN SPITE OF AN EIGHT-RESIDUE REDUNDANCY |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.86 Å3/Da / Density % sol: 56.93 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Details: THE MOTHER LIQUOR WAS 80 PERCENT SATURATED AMMONIUM SULFATE, PH 5.2. | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 5.2 / Method: macro seeding | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Observed criterion σ(F): 2 |
Reflection | *PLUS Highest resolution: 2 Å / Num. obs: 9604 / % possible obs: 84.8 % / Rmerge(I) obs: 0.078 |
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Processing
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Refinement | Resolution: 2→5 Å / Rfactor Rwork: 0.186 / Rfactor obs: 0.186 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→5 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR/PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Num. reflection obs: 9604 / σ(F): 2 / Rfactor obs: 0.186 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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