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Open data
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Basic information
Entry | Database: PDB / ID: 3sbj | ||||||
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Title | MutM slanted complex 7 | ||||||
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![]() | HYDROLASE/DNA / DNA glycosylase / DNA repair / damage search / translocation / disulfide crosslinking / HYDROLASE-DNA complex | ||||||
Function / homology | ![]() DNA-formamidopyrimidine glycosylase / 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / base-excision repair / damaged DNA binding / zinc ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sung, R.J. / Zhang, M. / Verdine, G.L. | ||||||
![]() | ![]() Title: Strandwise translocation of a DNA glycosylase on undamaged DNA. Authors: Qi, Y. / Nam, K. / Spong, M.C. / Banerjee, A. / Sung, R.J. / Zhang, M. / Karplus, M. / Verdine, G.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 146.4 KB | Display | ![]() |
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PDB format | ![]() | 111.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 449.4 KB | Display | ![]() |
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Full document | ![]() | 453.9 KB | Display | |
Data in XML | ![]() | 14.1 KB | Display | |
Data in CIF | ![]() | 19.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3sarC ![]() 3sasC ![]() 3satC ![]() 3sauC ![]() 3savC ![]() 3sawC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 30581.314 Da / Num. of mol.: 1 / Mutation: Q166C, V222P Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: MUTM / Plasmid: pET24B / Production host: ![]() ![]() |
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#2: DNA chain | Mass: 3423.248 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: synthetic DNA |
#3: DNA chain | Mass: 3344.282 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: synthetic DNA |
#4: Chemical | ChemComp-ZN / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.96 Å3/Da / Density % sol: 58.46 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7 Details: PEG 8K, sodium cacodylate, glycerol, pH 7.0, vapor diffusion, hanging drop, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Oct 28, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.078→34.2 Å / Num. obs: 26487 / Biso Wilson estimate: 28.97 Å2 |
Reflection shell | Highest resolution: 2.1 Å / Redundancy: 4.3 % / Mean I/σ(I) obs: 2.1 / Num. unique all: 1318 / Rsym value: 0.491 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 59.069 Å2 / ksol: 0.4 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 485.32 Å2 / Biso mean: 47.0561 Å2 / Biso min: 12.41 Å2
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Refinement step | Cycle: LAST / Resolution: 2.1→32.685 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 9
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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