+Open data
-Basic information
Entry | Database: PDB / ID: 3s6s | ||||||
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Title | Ancylostoma-secreted protein Ac-ASP-7 | ||||||
Components | Ac-ASP-7 | ||||||
Keywords | UNKNOWN FUNCTION / Pathogenesis-related Protein / SCP/TAPS | ||||||
Function / homology | Pathogenesis-related Protein p14a / CAP / 3-Layer(aba) Sandwich / Alpha Beta Function and homology information | ||||||
Biological species | Ancylostoma caninum (dog hookworm) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIRAS / Resolution: 2.4 Å | ||||||
Authors | Osman, A. / Wang, C.K. / Winter, A. / Loukas, A. / Tribolet, L. / Gasser, R. / Hofmann, A. | ||||||
Citation | Journal: Biotechnol Adv Title: Hookworm SCP/TAPS protein structure--A key to understanding host-parasite interactions and developing new interventions. Authors: Osman, A. / Wang, C.K. / Winter, A. / Loukas, A. / Tribolet, L. / Gasser, R.B. / Hofmann, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3s6s.cif.gz | 76.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3s6s.ent.gz | 61.2 KB | Display | PDB format |
PDBx/mmJSON format | 3s6s.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3s6s_validation.pdf.gz | 430.7 KB | Display | wwPDB validaton report |
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Full document | 3s6s_full_validation.pdf.gz | 436.8 KB | Display | |
Data in XML | 3s6s_validation.xml.gz | 15.8 KB | Display | |
Data in CIF | 3s6s_validation.cif.gz | 21.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/s6/3s6s ftp://data.pdbj.org/pub/pdb/validation_reports/s6/3s6s | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 23511.436 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Ancylostoma caninum (dog hookworm) / Production host: Pichia Pastoris (fungus) #2: Water | ChemComp-HOH / | Sequence details | AS PER THE THE AUTHORS THERE IS AN UNEXPLAINED SEQUENCE MISMATCH BETWEEN THE GENBANK SEQUENCE AND ...AS PER THE THE AUTHORS THERE IS AN UNEXPLAINE | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.37 Å3/Da / Density % sol: 48.12 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 20% MPD, 0.1 M TRIS, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX2 / Wavelength: 0.93224 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Nov 10, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.93224 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→61 Å / Num. obs: 18132 / % possible obs: 100 % / Redundancy: 7 % / Biso Wilson estimate: 44.5 Å2 / Rsym value: 0.074 |
Reflection shell | Resolution: 2.4→2.53 Å / Redundancy: 7.2 % / Mean I/σ(I) obs: 5.8 / Num. unique all: 2591 / Rsym value: 0.26 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MIRAS / Resolution: 2.4→60 Å / SU ML: 0.34 / σ(F): 1.46 / Phase error: 24.21 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.83 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 43.188 Å2 / ksol: 0.344 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.4→60 Å
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Refine LS restraints |
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LS refinement shell |
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