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Yorodumi- PDB-3s61: Reduced Form of Ornithine Hydroxylase (PvdA) from Pseudomonas aer... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3s61 | ||||||
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Title | Reduced Form of Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa | ||||||
Components | L-ornithine 5-monooxygenase | ||||||
Keywords | OXIDOREDUCTASE / N5-L-Ornithine monooxygenase / reduced form / class B flavin dependent N-hydroxylating monooxygenase / ornithine hydroxylase / bacterial cytosol | ||||||
Function / homology | Function and homology information L-ornithine N5-monooxygenase (NADPH) / ornithine N5-monooxygenase activity / pyoverdine biosynthetic process / intracellular iron ion homeostasis / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.03 Å | ||||||
Authors | Olucha, J. / Lamb, A.L. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2011 Title: Two Structures of an N-Hydroxylating Flavoprotein Monooxygenase: ORNITHINE HYDROXYLASE FROM PSEUDOMONAS AERUGINOSA. Authors: Olucha, J. / Meneely, K.M. / Chilton, A.S. / Lamb, A.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3s61.cif.gz | 180.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3s61.ent.gz | 142 KB | Display | PDB format |
PDBx/mmJSON format | 3s61.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/s6/3s61 ftp://data.pdbj.org/pub/pdb/validation_reports/s6/3s61 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Details | AUTHORS STATE THAT DYNAMIC LIGHT SCATTERING EXPERIMENTS HAVE SHOWN PVDA BEHAVES AS A MONOMER IN SOLUTION (MENEELY, K.M. AND LAMB, A.L., BIOCHEMISTRY, 2007, 46 (42), 11930-11937). |
-Components
#1: Protein | Mass: 51711.164 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Strain: PAO1 / Gene: PA2386, pvd-1, pvdA / Plasmid: pET28B / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) References: UniProt: Q51548, Oxidoreductases; Acting on single donors with incorporation of molecular oxygen (oxygenases); With incorporation of one atom of oxygen (internal monooxygenases or ...References: UniProt: Q51548, Oxidoreductases; Acting on single donors with incorporation of molecular oxygen (oxygenases); With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases) #2: Chemical | #3: Chemical | #4: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.14 Å3/Da / Density % sol: 60.85 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 5 Details: 9.5% PEG 8000, 25% glycerol, 60 mM potassium phosphate monobasic, Reducing agent Sodium Dithionite, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL9-2 / Wavelength: 0.9795 Å |
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Detector | Type: MARMOSAIC 325 mm CCD / Detector: CCD / Date: Mar 18, 2011 |
Radiation | Monochromator: Double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 3.03→39.26 Å / Num. all: 27042 / Num. obs: 26002 / % possible obs: 96 % |
Reflection shell | Resolution: 3.03→3.19 Å / Mean I/σ(I) obs: 5.8 / % possible all: 96 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: Oxidized form of PvdA Resolution: 3.03→39.26 Å / Cor.coef. Fo:Fc: 0.919 / Cor.coef. Fo:Fc free: 0.886 / SU B: 18.131 / SU ML: 0.332 / Cross valid method: THROUGHOUT / ESU R Free: 0.46 Stereochemistry target values: MAXIMUM LIKELIHOOD WITH PHASES Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 61.172 Å2
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Refinement step | Cycle: LAST / Resolution: 3.03→39.26 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.03→3.108 Å / Total num. of bins used: 20
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