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Yorodumi- PDB-3ruf: Alternative analogs as viable substrates of UDP-hexose 4-epimerases -
+Open data
-Basic information
Entry | Database: PDB / ID: 3ruf | ||||||
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Title | Alternative analogs as viable substrates of UDP-hexose 4-epimerases | ||||||
Components | WbgU | ||||||
Keywords | ISOMERASE / Rossmann fold / UDP-hexose 4-epimerase | ||||||
Function / homology | Function and homology information UDP-N-acetylglucosamine 4-epimerase / UDP-N-acetylglucosamine 4-epimerase activity / O antigen biosynthetic process / nucleotide binding Similarity search - Function | ||||||
Biological species | Plesiomonas shigelloides (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Bhatt, V.S. / Guan, W. / Wang, P.G. | ||||||
Citation | Journal: TO BE PUBLISHED Title: Alternative analogs as viable substrates of UDP-hexose 4-epimerases Authors: Bhatt, V.S. / Guan, W. / Wang, P.G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3ruf.cif.gz | 289 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3ruf.ent.gz | 233.5 KB | Display | PDB format |
PDBx/mmJSON format | 3ruf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3ruf_validation.pdf.gz | 2.9 MB | Display | wwPDB validaton report |
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Full document | 3ruf_full_validation.pdf.gz | 3 MB | Display | |
Data in XML | 3ruf_validation.xml.gz | 53.4 KB | Display | |
Data in CIF | 3ruf_validation.cif.gz | 73.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ru/3ruf ftp://data.pdbj.org/pub/pdb/validation_reports/ru/3ruf | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 39822.109 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Plesiomonas shigelloides (bacteria) / Gene: wbgU / Plasmid: pET-15b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q7BJX9 #2: Chemical | ChemComp-NAD / #3: Chemical | ChemComp-UDP / #4: Chemical | ChemComp-SO4 / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.47 Å3/Da / Density % sol: 50.14 % |
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Crystal grow | Temperature: 300 K / Method: microbatch under oil / pH: 6.5 Details: 25% PEG 3350, 0.2 M Ammonium Sulfate, 200 mM Bis Tris Propane pH 6.5 , microbatch under oil, temperature 300K |
-Data collection
Diffraction | Mean temperature: 103 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.98 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Aug 14, 2010 |
Radiation | Monochromator: Si / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 2→37 Å / Num. obs: 96669 / % possible obs: 91 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2→37 Å / Cor.coef. Fo:Fc: 0.954 / Cor.coef. Fo:Fc free: 0.935 / SU B: 4.578 / SU ML: 0.129 / Cross valid method: THROUGHOUT / ESU R Free: 0.174 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 43.218 Å2
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Refinement step | Cycle: LAST / Resolution: 2→37 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.052 Å / Total num. of bins used: 20
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