+Open data
-Basic information
Entry | Database: PDB / ID: 3qax | ||||||
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Title | Crystal structure analysis of the cpb0502 | ||||||
Components | Probable ABC transporter arginine-binding protein ArtJ | ||||||
Keywords | TRANSPORT PROTEIN / ABC transporter / arginine binding / periplasmic | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Chlamydophila pneumoniae (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Chang, J.E. / Hwang, K.Y. | ||||||
Citation | Journal: To be Published Title: Crystal structure of the cpb0502 Authors: Chang, J.E. / Hwang, K.Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3qax.cif.gz | 115.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3qax.ent.gz | 88.8 KB | Display | PDB format |
PDBx/mmJSON format | 3qax.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3qax_validation.pdf.gz | 460.1 KB | Display | wwPDB validaton report |
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Full document | 3qax_full_validation.pdf.gz | 473 KB | Display | |
Data in XML | 3qax_validation.xml.gz | 25.8 KB | Display | |
Data in CIF | 3qax_validation.cif.gz | 38.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qa/3qax ftp://data.pdbj.org/pub/pdb/validation_reports/qa/3qax | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 30064.754 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chlamydophila pneumoniae (bacteria) / Gene: artJ, CPn_0482, CP_0272, CPj0482, CpB0502 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9Z869 #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.08 Å3/Da / Density % sol: 40.75 % |
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Crystal grow | Temperature: 297 K / Method: vapor diffusion, hanging drop / pH: 8 Details: sodium acetate, 20% PEG 3350, 1,5-Diaminopentane dihydrochloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 297KK |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: PAL/PLS / Beamline: 4A / Wavelength: 0.99188 Å |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Apr 20, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.99188 Å / Relative weight: 1 |
Reflection | Resolution: 2→29.96 Å / Num. obs: 42387 / % possible obs: 93.26 % / Observed criterion σ(F): 1.2 / Observed criterion σ(I): 1.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2→29.92 Å / Cor.coef. Fo:Fc: 0.931 / Cor.coef. Fo:Fc free: 0.878 / SU B: 4.669 / SU ML: 0.13 / Cross valid method: THROUGHOUT / ESU R Free: 0.196 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 15.211 Å2
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Refinement step | Cycle: LAST / Resolution: 2→29.92 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.052 Å / Total num. of bins used: 20
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