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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 3pow | ||||||
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タイトル | Crystal structure of the globular domain of human calreticulin | ||||||
![]() | calreticulin | ||||||
![]() | CHAPERONE / legume lectin fold / CNX/CRT family / multi-functional / Carbohydrate binding / Peptide Binding / multi-compartmental | ||||||
機能・相同性 | ![]() Calnexin/calreticulin cycle / cytolytic granule / positive regulation of dendritic cell chemotaxis / cortical granule / Assembly of Viral Components at the Budding Site / negative regulation of trophoblast cell migration / ATF6 (ATF6-alpha) activates chaperone genes / negative regulation of retinoic acid receptor signaling pathway / cellular response to electrical stimulus / endoplasmic reticulum quality control compartment ...Calnexin/calreticulin cycle / cytolytic granule / positive regulation of dendritic cell chemotaxis / cortical granule / Assembly of Viral Components at the Budding Site / negative regulation of trophoblast cell migration / ATF6 (ATF6-alpha) activates chaperone genes / negative regulation of retinoic acid receptor signaling pathway / cellular response to electrical stimulus / endoplasmic reticulum quality control compartment / nuclear receptor-mediated glucocorticoid signaling pathway / regulation of meiotic nuclear division / complement component C1q complex binding / sequestering of calcium ion / response to glycoside / sarcoplasmic reticulum lumen / protein folding in endoplasmic reticulum / nuclear export signal receptor activity / negative regulation of intracellular steroid hormone receptor signaling pathway / hormone binding / cardiac muscle cell differentiation / molecular sequestering activity / protein maturation by protein folding / Scavenging by Class F Receptors / Scavenging by Class A Receptors / cortical actin cytoskeleton organization / nuclear androgen receptor binding / cellular response to lithium ion / response to testosterone / protein localization to nucleus / negative regulation of neuron differentiation / smooth endoplasmic reticulum / positive regulation of cell cycle / ERAD pathway / positive regulation of substrate adhesion-dependent cell spreading / protein folding chaperone / positive regulation of phagocytosis / endocytic vesicle lumen / protein export from nucleus / positive regulation of endothelial cell migration / endoplasmic reticulum-Golgi intermediate compartment membrane / acrosomal vesicle / lumenal side of endoplasmic reticulum membrane / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / peptide binding / peptide antigen assembly with MHC class I protein complex / MHC class I peptide loading complex / cellular response to virus / intracellular calcium ion homeostasis / positive regulation of non-canonical NF-kappaB signal transduction / phagocytic vesicle membrane / cellular senescence / unfolded protein binding / integrin binding / protein folding / response to estradiol / nuclear envelope / ER-Phagosome pathway / protein-folding chaperone binding / carbohydrate binding / spermatogenesis / regulation of apoptotic process / collagen-containing extracellular matrix / protein stabilization / negative regulation of translation / ribosome / response to xenobiotic stimulus / iron ion binding / endoplasmic reticulum lumen / external side of plasma membrane / focal adhesion / negative regulation of DNA-templated transcription / mRNA binding / ubiquitin protein ligase binding / calcium ion binding / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of DNA-templated transcription / endoplasmic reticulum membrane / perinuclear region of cytoplasm / negative regulation of transcription by RNA polymerase II / cell surface / endoplasmic reticulum / DNA binding / RNA binding / extracellular space / zinc ion binding / extracellular exosome / extracellular region / membrane / nucleus / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Gaboriaud, C. | ||||||
![]() | ![]() タイトル: X-ray structure of the human calreticulin globular domain reveals a Peptide-binding area and suggests a multi-molecular mechanism 著者: Chouquet, A. / Paidassi, H. / Ling, W.L. / Frachet, P. / Houen, G. / Arlaud, G.J. / Gaboriaud, C. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 121.6 KB | 表示 | ![]() |
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PDB形式 | ![]() | 90.9 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 418.6 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 418.8 KB | 表示 | |
XML形式データ | ![]() | 14.2 KB | 表示 | |
CIF形式データ | ![]() | 21.7 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 30146.271 Da / 分子数: 1 断片: Globular domain, UNP residues 18-204 and 302-368 linked with GSG 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
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#2: 化合物 | ChemComp-CA / |
#3: 水 | ChemComp-HOH / |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.3 Å3/Da / 溶媒含有率: 46.49 % |
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結晶化 | 温度: 277.1 K / 手法: 蒸気拡散法 / pH: 6 詳細: 30% PEG 4000, 0.2M ammonium acetate, 10mM magnesium acetate, and either 0.05M MES (pH 5.7 or 6.0), or 0.05M HEPES (pH 7.0), VAPOR DIFFUSION, temperature 277.1K |
-データ収集
回折 | 平均測定温度: 100 K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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放射光源 | 由来: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
検出器 | タイプ: MARMOSAIC 225 mm CCD / 検出器: CCD / 日付: 2010年4月24日 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
放射 | モノクロメーター: horizontally side diffracting Silicon 111 crystal プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
放射波長 | 波長: 0.8726 Å / 相対比: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | 数: 6705 / D res high: 1.54 Å / Num. obs: 6705 / % possible obs: 2.1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Diffraction reflection shell |
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反射 | 解像度: 1.54→20 Å / Num. all: 39833 / Num. obs: 39833 / % possible obs: 96.8 % / Observed criterion σ(I): -3 / 冗長度: 4.7 % / Biso Wilson estimate: 20.093 Å2 / Rsym value: 0.07 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
反射 シェル |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: PDB ENTRY 3POS 解像度: 1.55→10 Å / Cor.coef. Fo:Fc: 0.9472 / Cor.coef. Fo:Fc free: 0.9465 / Occupancy max: 1 / Occupancy min: 0.5 / 交差検証法: THROUGHOUT / σ(F): 0 / 立体化学のターゲット値: Engh & Huber
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原子変位パラメータ | Biso max: 78.04 Å2 / Biso mean: 16.0774 Å2 / Biso min: 5.52 Å2
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Refine analyze | Luzzati coordinate error obs: 0.187 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 1.55→10 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 1.55→1.59 Å / Total num. of bins used: 20
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精密化 TLS | 手法: refined / Origin x: 2.1858 Å / Origin y: 10.1515 Å / Origin z: 15.0518 Å
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精密化 TLSグループ |
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