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Open data
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Basic information
Entry | Database: PDB / ID: 3piw | ||||||
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Title | Zebrafish interferon 2 | ||||||
![]() | Type I interferon 2 | ||||||
![]() | CYTOKINE / Interferon / zebrafish / interleukin | ||||||
Function / homology | ![]() cytokine receptor binding / cytokine activity / defense response to virus / extracellular space Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hamming, O.J. / Hartmann, R. / Lutfalla, G. / Levraud, J.-P. | ||||||
![]() | ![]() Title: Crystal Structure of Zebrafish Interferons I and II Reveals Conservation of Type I Interferon Structure in Vertebrates. Authors: Hamming, O.J. / Lutfalla, G. / Levraud, J.P. / Hartmann, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 85.1 KB | Display | ![]() |
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PDB format | ![]() | 69 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 422.8 KB | Display | ![]() |
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Full document | ![]() | 425.7 KB | Display | |
Data in XML | ![]() | 11.5 KB | Display | |
Data in CIF | ![]() | 17.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 18661.633 Da / Num. of mol.: 1 / Mutation: F27I,L46I,F89V,Q120K Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.4 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 8 Details: 0.2 M MgCl2, 22% PEG3350, 0.1 M HEPES pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
-Data collection
Diffraction |
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Jan 1, 2008 | |||||||||
Radiation | Monochromator: Double crystal monochromator, Si(111). / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 1.49→72.932 Å / Num. all: 30020 / Num. obs: 29444 / % possible obs: 98.44 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 | |||||||||
Reflection shell | Resolution: 1.4918→1.5399 Å / % possible all: 87 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 58.003 Å2 / ksol: 0.362 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.492→30.813 Å
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Refine LS restraints |
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LS refinement shell |
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