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-基本情報
登録情報 | データベース: PDB / ID: 3pe0 | ||||||
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タイトル | Structure of the central region of the plakin domain of plectin | ||||||
要素 | Plectin | ||||||
キーワード | STRUCTURAL PROTEIN / cytoskeleton / plakin / spectrin repeat / SH3 / intermediate filament / crosslinking | ||||||
機能・相同性 | 機能・相同性情報 leukocyte migration involved in immune response / protein-containing complex organization / actomyosin contractile ring assembly actin filament organization / skeletal myofibril assembly / tight junction organization / Type I hemidesmosome assembly / hemidesmosome assembly / hemidesmosome / intermediate filament organization / intermediate filament cytoskeleton organization ...leukocyte migration involved in immune response / protein-containing complex organization / actomyosin contractile ring assembly actin filament organization / skeletal myofibril assembly / tight junction organization / Type I hemidesmosome assembly / hemidesmosome assembly / hemidesmosome / intermediate filament organization / intermediate filament cytoskeleton organization / regulation of vascular permeability / dystroglycan binding / cellular response to hydrostatic pressure / fibroblast migration / T cell chemotaxis / costamere / cellular response to fluid shear stress / peripheral nervous system myelin maintenance / podosome / adherens junction organization / cardiac muscle cell development / myoblast differentiation / structural constituent of muscle / intermediate filament cytoskeleton / response to food / ankyrin binding / Assembly of collagen fibrils and other multimeric structures / sarcomere organization / nucleus organization / keratinocyte development / transmission of nerve impulse / brush border / sarcoplasm / establishment of skin barrier / Caspase-mediated cleavage of cytoskeletal proteins / skeletal muscle fiber development / respiratory electron transport chain / mitochondrion organization / wound healing / cell morphogenesis / multicellular organism growth / sarcolemma / Z disc / structural constituent of cytoskeleton / cellular response to mechanical stimulus / actin filament binding / protein localization / myelin sheath / gene expression / mitochondrial outer membrane / cadherin binding / axon / focal adhesion / dendrite / perinuclear region of cytoplasm / RNA binding / extracellular exosome / plasma membrane / cytosol / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | X線回折 / 単一同系置換・異常分散 / 解像度: 2.95 Å | ||||||
データ登録者 | Ortega, E. / de Pereda, J.M. | ||||||
引用 | ジャーナル: J Biol Chem / 年: 2011 タイトル: The structure of the plakin domain of plectin reveals a non-canonical SH3 domain interacting with its fourth spectrin repeat. 著者: Esther Ortega / Rubén M Buey / Arnoud Sonnenberg / José M de Pereda / 要旨: Plectin belongs to the plakin family of cytoskeletal crosslinkers, which is part of the spectrin superfamily. Plakins contain an N-terminal conserved region, the plakin domain, which is formed by an ...Plectin belongs to the plakin family of cytoskeletal crosslinkers, which is part of the spectrin superfamily. Plakins contain an N-terminal conserved region, the plakin domain, which is formed by an array of spectrin repeats (SR) and a Src-homology 3 (SH3), and harbors binding sites for junctional proteins. We have combined x-ray crystallography and small angle x-ray scattering (SAXS) to elucidate the structure of the central region of the plakin domain of plectin, which corresponds to the SR3, SR4, SR5, and SH3 domains. The crystal structures of the SR3-SR4 and SR4-SR5-SH3 fragments were determined to 2.2 and 2.95 Å resolution, respectively. The SH3 of plectin presents major alterations as compared with canonical Pro-rich binding SH3 domains, suggesting that plectin does not recognize Pro-rich motifs. In addition, the SH3 binding site is partially occluded by an intramolecular contact with the SR4. Residues of this pseudo-binding site and the SR4/SH3 interface are conserved within the plakin family, suggesting that the structure of this part of the plectin molecule is similar to that of other plakins. We have created a model for the SR3-SR4-SR5-SH3 region, which agrees well with SAXS data in solution. The three SRs form a semi-flexible rod that is not altered by the presence of the SH3 domain, and it is similar to those found in spectrins. The flexibility of the plakin domain, in analogy with spectrins, might contribute to the role of plakins in maintaining the stability of tissues subject to mechanical stress. | ||||||
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 3pe0.cif.gz | 230.8 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb3pe0.ent.gz | 189.8 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 3pe0.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 3pe0_validation.pdf.gz | 431.3 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 3pe0_full_validation.pdf.gz | 436.4 KB | 表示 | |
XML形式データ | 3pe0_validation.xml.gz | 20.3 KB | 表示 | |
CIF形式データ | 3pe0_validation.cif.gz | 26.7 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/pe/3pe0 ftp://data.pdbj.org/pub/pdb/validation_reports/pe/3pe0 | HTTPS FTP |
-関連構造データ
-リンク
-集合体
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非結晶学的対称性 (NCS) | NCSドメイン:
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