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- PDB-3p8m: Human dynein light chain (DYNLL2) in complex with an in vitro evo... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3p8m | ||||||
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Title | Human dynein light chain (DYNLL2) in complex with an in vitro evolved peptide dimerized by leucine zipper | ||||||
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![]() | PROTEIN BINDING / Phage display / Leucine zipper / Hub protein | ||||||
Function / homology | ![]() myosin V complex / Activation of BMF and translocation to mitochondria / 9+0 non-motile cilium / ciliary tip / Intraflagellar transport / protein localization to nuclear periphery / FCERI mediated MAPK activation / Activation of the AP-1 family of transcription factors / response to amino acid starvation / mediator complex binding ...myosin V complex / Activation of BMF and translocation to mitochondria / 9+0 non-motile cilium / ciliary tip / Intraflagellar transport / protein localization to nuclear periphery / FCERI mediated MAPK activation / Activation of the AP-1 family of transcription factors / response to amino acid starvation / mediator complex binding / negative regulation of ribosomal protein gene transcription by RNA polymerase II / positive regulation of cellular response to amino acid starvation / COPI-independent Golgi-to-ER retrograde traffic / nitrogen catabolite activation of transcription from RNA polymerase II promoter / cytoplasmic dynein complex / Oxidative Stress Induced Senescence / dynein intermediate chain binding / Macroautophagy / microtubule-based process / TFIID-class transcription factor complex binding / positive regulation of transcription initiation by RNA polymerase II / positive regulation of RNA polymerase II transcription preinitiation complex assembly / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / COPI-mediated anterograde transport / amino acid biosynthetic process / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Resolution of Sister Chromatid Cohesion / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / MHC class II antigen presentation / cellular response to amino acid starvation / RHO GTPases Activate Formins / cilium / Aggrephagy / HCMV Early Events / RNA polymerase II transcription regulator complex / Separation of Sister Chromatids / : / postsynapse / DNA-binding transcription activator activity, RNA polymerase II-specific / microtubule / RNA polymerase II-specific DNA-binding transcription factor binding / transcription regulator complex / sequence-specific DNA binding / cytoskeleton / DNA-binding transcription factor activity, RNA polymerase II-specific / regulation of cell cycle / intracellular signal transduction / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / centrosome / glutamatergic synapse / chromatin binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / identical protein binding / membrane / nucleus / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Rapali, P. / Radnai, L. / Suveges, D. / Hetenyi, C. / Harmat, V. / Tolgyesi, F. / Wahlgren, W.Y. / Katona, G. / Nyitray, L. / Pal, G. | ||||||
![]() | ![]() Title: Directed evolution reveals the binding motif preference of the LC8/DYNLL hub protein and predicts large numbers of novel binders in the human proteome. Authors: Rapali, P. / Radnai, L. / Suveges, D. / Harmat, V. / Tolgyesi, F. / Wahlgren, W.Y. / Katona, G. / Nyitray, L. / Pal, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 116.9 KB | Display | ![]() |
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PDB format | ![]() | 91.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 456.3 KB | Display | ![]() |
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Full document | ![]() | 458.8 KB | Display | |
Data in XML | ![]() | 11.5 KB | Display | |
Data in CIF | ![]() | 15.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2xqqSC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 1 / Refine code: 3
NCS ensembles :
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Components
#1: Protein | Mass: 10647.124 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein/peptide | Mass: 5110.736 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: GCN4, AAS3, ARG9, YEL009C / Plasmid: modified pET / Production host: ![]() ![]() #3: Water | ChemComp-HOH / | Sequence details | THE IN VITRO EVOLVED BINDING SEQUENCE. | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.97 Å3/Da / Density % sol: 58.6 % |
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Crystal grow | Temperature: 293 K / pH: 7 Details: 20% PEG8000, 0.2 M MgCl2, 0.1 M TRIS, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Mar 1, 2010 / Details: OSMIC BLUE |
Radiation | Monochromator: CONFOCAL MIRRORS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→56.76 Å / Num. obs: 8273 / % possible obs: 94.4 % / Observed criterion σ(I): -3 / Redundancy: 3.23 % / Rmerge(I) obs: 0.131 / Net I/σ(I): 6.4 |
Reflection shell | Resolution: 2.9→3 Å / Redundancy: 3.12 % / Rmerge(I) obs: 0.576 / Mean I/σ(I) obs: 1.5 / % possible all: 96.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2XQQ Resolution: 2.9→42.31 Å / Cor.coef. Fo:Fc: 0.915 / Cor.coef. Fo:Fc free: 0.894 / SU B: 46.408 / SU ML: 0.391 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.451 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 52.8 Å2
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Refinement step | Cycle: LAST / Resolution: 2.9→42.31 Å
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Refine LS restraints |
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Refine LS restraints NCS | Refine-ID: X-RAY DIFFRACTION
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LS refinement shell | Resolution: 2.9→2.98 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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