温度: 277 K / 手法: 蒸気拡散法, シッティングドロップ法 詳細: equal volumes SeMet protein solution (24 mg/ml LmTyrRS in MSGPP buffer containing 5 mM DTT and 5 mM fisetin) and well solution (25% PEG 3350, 0.1 M tri-sodium citrate pH 5.5, and 4% w/v ...詳細: equal volumes SeMet protein solution (24 mg/ml LmTyrRS in MSGPP buffer containing 5 mM DTT and 5 mM fisetin) and well solution (25% PEG 3350, 0.1 M tri-sodium citrate pH 5.5, and 4% w/v acetone); cryoprotected by addition of 30% PEG 3350, 10% MSGPP buffer, 10% ethylene glycol, and 0.1 M tri-sodium citrate pH 5.5 directly to drop prior to mounting and freezing crystals in liquid nitrogen, VAPOR DIFFUSION, SITTING DROP, temperature 277K
構造決定の手法: 分子置換, 単波長異常分散 開始モデル: Initial partial MR solution with PDB entry 2j5b, then determined experimental phases and heavy atom positions by SAD. Also used PDB entries 2cya and 2cyc to aid in model completion.