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Yorodumi- PDB-3nvm: Structural basis for substrate placement by an archaeal box C/D r... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3nvm | ||||||
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Title | Structural basis for substrate placement by an archaeal box C/D ribonucleoprotein particle | ||||||
Components |
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Keywords | TRANSFERASE / Nop domain / methyltransferase / ribosome biogenesis / spliceosome biogenesis | ||||||
Function / homology | Function and homology information box C/D methylation guide snoRNP complex / tRNA processing / snoRNA binding / Transferases; Transferring one-carbon groups; Methyltransferases / small-subunit processome / methyltransferase activity / rRNA processing / methylation / RNA binding Similarity search - Function | ||||||
Biological species | Pyrococcus furiosus (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.408 Å | ||||||
Authors | Xue, S. / Wang, R. / Li, H. | ||||||
Citation | Journal: Mol.Cell / Year: 2010 Title: Structural basis for substrate placement by an archaeal box C/D ribonucleoprotein particle. Authors: Xue, S. / Wang, R. / Yang, F. / Terns, R.M. / Terns, M.P. / Zhang, X. / Maxwell, E.S. / Li, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3nvm.cif.gz | 249 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3nvm.ent.gz | 204.5 KB | Display | PDB format |
PDBx/mmJSON format | 3nvm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nv/3nvm ftp://data.pdbj.org/pub/pdb/validation_reports/nv/3nvm | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 42946.410 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pyrococcus furiosus (archaea) / Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1 / Gene: PF0060 / Production host: Escherichia coli (E. coli) / References: UniProt: Q8U4M1 |
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#2: Protein | Mass: 26760.844 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pyrococcus furiosus (archaea) / Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1 / Gene: flpA, PF0059 / Production host: Escherichia coli (E. coli) References: UniProt: Q8U4M2, Transferases; Transferring one-carbon groups; Methyltransferases |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 5.56 Å3/Da / Density % sol: 77.89 % |
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Crystal grow | Temperature: 303 K / Method: vapor diffusion, hanging drop / pH: 6.4 Details: 0.1 M imidazole pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
-Data collection
Diffraction |
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Diffraction source |
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Detector |
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Radiation | Monochromator: 1 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength | Relative weight: 1 | ||||||||||||
Reflection | Resolution: 3.4→50 Å / Num. all: 35269 / Num. obs: 32901 / % possible obs: 80 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 | ||||||||||||
Reflection shell | Resolution: 3.4→50 Å / % possible all: 26 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.408→49.431 Å / SU ML: 0.51 / σ(F): 1.91 / Phase error: 30.88 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 191.675 Å2 / ksol: 0.347 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 3.408→49.431 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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