- PDB-3nct: X-ray crystal structure of the bacterial conjugation factor PsiB,... -
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Open data
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Basic information
Entry
Database: PDB / ID: 3nct
Title
X-ray crystal structure of the bacterial conjugation factor PsiB, a negative regulator of reca
Components
Protein psiB
Keywords
DNA BINDING PROTEIN / CHAPERONE
Function / homology
Plasmid SOS inhibition protein / Protein PsiB / Protein PsiB-like superfamily / Plasmid SOS inhibition protein (PsiB) / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta / Protein PsiB
Function and homology information
Biological species
Escherichia coli (E. coli)
Method
X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.2 Å
Mass: 18.015 Da / Num. of mol.: 258 / Source method: isolated from a natural source / Formula: H2O
Sequence details
THE AUTHORS CLAIM THAT THE UNIPROT ENTRY P10031 CONTAINS AN INCORRECT AMINOACID AT POSITION 137. ...THE AUTHORS CLAIM THAT THE UNIPROT ENTRY P10031 CONTAINS AN INCORRECT AMINOACID AT POSITION 137. THE CORRECT FULL SEQUENCE IS REPORTED IN THE PAPER BY DUTREIX M. ET AL. (NUCLEIC ACIDS RESEARCH 1988, V. 16, PAGES:10669-10679). SEE ALSO REMARK SEQADV.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.81 Å3/Da / Density % sol: 56.24 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.2 Details: 136 mM sodium acetate, pH 5.2, 1.4% PEG 4000, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
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