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- PDB-3n83: T244A mutant of human mitochondrial aldehyde dehydrogenase, NAD c... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3n83 | ||||||
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Title | T244A mutant of human mitochondrial aldehyde dehydrogenase, NAD complex | ||||||
![]() | Aldehyde dehydrogenase, mitochondrial | ||||||
![]() | OXIDOREDUCTASE / ALDH / Rossmann fold | ||||||
Function / homology | ![]() Metabolism of serotonin / : / regulation of dopamine biosynthetic process / regulation of serotonin biosynthetic process / aldehyde catabolic process / phenylacetaldehyde dehydrogenase (NAD+) activity / alcohol metabolic process / aldehyde dehydrogenase [NAD(P)+] activity / ethanol catabolic process / Ethanol oxidation ...Metabolism of serotonin / : / regulation of dopamine biosynthetic process / regulation of serotonin biosynthetic process / aldehyde catabolic process / phenylacetaldehyde dehydrogenase (NAD+) activity / alcohol metabolic process / aldehyde dehydrogenase [NAD(P)+] activity / ethanol catabolic process / Ethanol oxidation / carboxylesterase activity / glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity / aldehyde dehydrogenase (NAD+) / aldehyde dehydrogenase (NAD+) activity / Smooth Muscle Contraction / Mitochondrial protein degradation / NAD binding / carbohydrate metabolic process / electron transfer activity / mitochondrial matrix / mitochondrion / extracellular exosome Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Gonzalez-Segura, L. / Hurley, T.D. | ||||||
![]() | ![]() Title: Conformational Selection During Catalysis: The role of Threonine 244 in ALDH2 Authors: Ho, K.-K. / Gonzalez-Segura, L. / Perez-Miller, S. / Weiner, H. / Hurley, T.D. #1: ![]() Title: Selective Alteration of the Rate-Limiting Step in Cytosolic Aldehyde Dehydrogenase through Random Mutagenesis Authors: Ho, K.-K. / Hurley, T.D. / Weiner, H. #2: ![]() Title: Structural and Functional Consequences of Coenzyme Binding to the Inactive Asian Variant of Mitochondrial Aldehyde Dehydrogenase (ROLES OF RESIDUES 475 AND 487) Authors: Larson, H.N. / Zhou, J. / Chen, Z. / Stamler, J.S. / Weiner, H. / Hurley, T.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 819.7 KB | Display | ![]() |
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PDB format | ![]() | 672 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 5 MB | Display | ![]() |
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Full document | ![]() | 5 MB | Display | |
Data in XML | ![]() | 161.2 KB | Display | |
Data in CIF | ![]() | 234.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3n80C ![]() 3n81C ![]() 3n82C ![]() 1o05S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
-Protein , 1 types, 8 molecules ABCDEFGH
#1: Protein | Mass: 54469.602 Da / Num. of mol.: 8 / Fragment: Mature sequence, residues 18-517 / Mutation: T244A Source method: isolated from a genetically manipulated source Details: lacks mitochondrial leader sequence / Source: (gene. exp.) ![]() ![]() ![]() |
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-Non-polymers , 5 types, 2988 molecules ![](data/chem/img/NA.gif)
![](data/chem/img/GAI.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/ADP.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/GAI.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/ADP.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-NA / #3: Chemical | ChemComp-GAI / #4: Chemical | ChemComp-EDO / #5: Chemical | ChemComp-ADP / #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.94 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion / pH: 6.4 Details: 100 MM ACES (N-[2-ACETAMIDO]-2-AMINOETHANE SULFONIC ACID), 1-10MM MGCL2, 100-200 MM GUANIDINE HCL, 16-17% W/V PEG 6000, pH 6.4, vapor diffusion, temperature 292K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jul 12, 2007 |
Radiation | Monochromator: SAGITALLY FOCUSED Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→50 Å / Num. obs: 305300 / % possible obs: 99.8 % / Redundancy: 6 % / Biso Wilson estimate: 30.1 Å2 / Rmerge(I) obs: 0.087 / Net I/σ(I): 20.6 |
Reflection shell | Resolution: 1.9→1.97 Å / Redundancy: 5.7 % / Rmerge(I) obs: 0.539 / Mean I/σ(I) obs: 2.9 / % possible all: 99.7 |
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Processing
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Refinement | Method to determine structure: Direct Refinement Starting model: PDB Entry 1o05 Resolution: 1.9→44.06 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.934 / Occupancy max: 1 / Occupancy min: 0.25 / SU B: 4.261 / SU ML: 0.122 / Cross valid method: THROUGHOUT / ESU R Free: 0.154 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 56.76 Å2 / Biso mean: 21.221 Å2 / Biso min: 6.58 Å2
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Refinement step | Cycle: LAST / Resolution: 1.9→44.06 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.901→1.95 Å / Total num. of bins used: 20
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