+Open data
-Basic information
Entry | Database: PDB / ID: 3mx7 | ||||||
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Title | Crystal Structure Analysis of Human FAIM-NTD | ||||||
Components | Fas apoptotic inhibitory molecule 1 | ||||||
Keywords | APOPTOSIS / Beta Sheet | ||||||
Function / homology | Function and homology information positive regulation of neurogenesis / canonical NF-kappaB signal transduction / negative regulation of extrinsic apoptotic signaling pathway via death domain receptors / apoptotic process / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 1.76 Å | ||||||
Authors | Li, G. / Qu, L. / Meng, G. / Zheng, X. | ||||||
Citation | Journal: To be Published Title: The structure of human Fas apoptotic inhibitory molecule Authors: Li, G. / Qu, L. / Meng, G. / Bai, X. / Dai, K. / Zheng, X. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3mx7.cif.gz | 30.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3mx7.ent.gz | 20.7 KB | Display | PDB format |
PDBx/mmJSON format | 3mx7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3mx7_validation.pdf.gz | 420.9 KB | Display | wwPDB validaton report |
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Full document | 3mx7_full_validation.pdf.gz | 421 KB | Display | |
Data in XML | 3mx7_validation.xml.gz | 6.7 KB | Display | |
Data in CIF | 3mx7_validation.cif.gz | 8.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mx/3mx7 ftp://data.pdbj.org/pub/pdb/validation_reports/mx/3mx7 | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 10183.710 Da / Num. of mol.: 1 / Fragment: N-TERMINAL DOMAIN, UNP residues 1-90 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: FAIM, FAIM1 / Plasmid: pET28a / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q9NVQ4 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.24 Å3/Da / Density % sol: 62.04 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 0.67M sodium phosphate, 0.73M potassium phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 1.04361 Å |
Detector | Type: SBC-2 / Detector: CCD / Date: Jan 16, 2010 |
Radiation | Monochromator: SAGITALLY FOCUSED Si / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.04361 Å / Relative weight: 1 |
Reflection | Resolution: 1.76→50 Å / Num. obs: 14391 / % possible obs: 99.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 5.7 % / Biso Wilson estimate: 11.6 Å2 / Rmerge(I) obs: 0.069 / Rsym value: 0.069 / Net I/σ(I): 17.267 |
Reflection shell | Resolution: 1.76→1.82 Å / Redundancy: 5 % / Rmerge(I) obs: 0.187 / Mean I/σ(I) obs: 11.041 / Num. unique all: 2521 / Rsym value: 0.187 / % possible all: 99.4 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 1.76→28.57 Å / Cor.coef. Fo:Fc: 0.957 / Cor.coef. Fo:Fc free: 0.944 / SU B: 1.687 / SU ML: 0.055 / Cross valid method: THROUGHOUT / σ(F): 0.16 / ESU R Free: 0.091 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 20.386 Å2
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Refinement step | Cycle: LAST / Resolution: 1.76→28.57 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.76→1.805 Å / Total num. of bins used: 20
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