+Open data
-Basic information
Entry | Database: PDB / ID: 1no8 | ||||||
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Title | SOLUTION STRUCTURE OF THE NUCLEAR FACTOR ALY RBD DOMAIN | ||||||
Components | ALY | ||||||
Keywords | RNA BINDING PROTEIN / RBD / ALY / REF1-I / BEF / mRNA EXPORT FACTOR | ||||||
Function / homology | Function and homology information mRNA 3'-end processing / RNA Polymerase II Transcription Termination / Transport of Mature mRNA derived from an Intron-Containing Transcript / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA / Transport of Mature mRNA Derived from an Intronless Transcript / C5-methylcytidine-containing RNA reader activity / mRNA Splicing - Major Pathway / RNA export from nucleus / mRNA transport ...mRNA 3'-end processing / RNA Polymerase II Transcription Termination / Transport of Mature mRNA derived from an Intron-Containing Transcript / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA / Transport of Mature mRNA Derived from an Intronless Transcript / C5-methylcytidine-containing RNA reader activity / mRNA Splicing - Major Pathway / RNA export from nucleus / mRNA transport / RNA splicing / spliceosomal complex / mRNA processing / single-stranded DNA binding / nuclear speck / RNA binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / simulated annealing torsion angle dynamics molecular dynamics | ||||||
Authors | Perez-Alvarado, G.C. / Martinez-Yamout, M. / Allen, M.M. / Grosschedl, R. / Dyson, H.J. / Wright, P.E. | ||||||
Citation | Journal: Biochemistry / Year: 2003 Title: Structure of the Nuclear Factor ALY: Insights into Post-Transcriptional Regulatory and mRNA Nuclear Export Processes Authors: Perez-Alvarado, G.C. / Martinez-Yamout, M. / Allen, M.M. / Grosschedl, R. / Dyson, H.J. / Wright, P.E. | ||||||
History |
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Remark 650 | HELIX Author determined the helix structure. | ||||||
Remark 700 | SHEET Author determined the sheet records. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1no8.cif.gz | 748.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1no8.ent.gz | 634.3 KB | Display | PDB format |
PDBx/mmJSON format | 1no8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1no8_validation.pdf.gz | 349.4 KB | Display | wwPDB validaton report |
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Full document | 1no8_full_validation.pdf.gz | 508.9 KB | Display | |
Data in XML | 1no8_validation.xml.gz | 32.6 KB | Display | |
Data in CIF | 1no8_validation.cif.gz | 57.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/no/1no8 ftp://data.pdbj.org/pub/pdb/validation_reports/no/1no8 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11508.897 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: ALY / Plasmid: pET21a / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: O08583 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using restraints derived from a combination of NOESY spectra and triple resonance spectra. |
-Sample preparation
Details |
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Sample conditions |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing torsion angle dynamics molecular dynamics Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. RESIDUES 77-104 ARE HIGHLY DISORDERED AND DO NOT PRESENT A DEFINED STRUCTURE IN SOLUTION. RESIDUES 77-104 ARE NOT INCLUDED IN THIS ENTRY. | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: Structures with the lowest energy and lowest constraint energy Conformers calculated total number: 92 / Conformers submitted total number: 32 |