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- PDB-3maa: Complex of GS-Alpha with the Catalytic Domains of Mammalian Adeny... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3maa | |||||||||
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Title | Complex of GS-Alpha with the Catalytic Domains of Mammalian Adenylyl Cyclase: Complex with Adenosine 5-O-(l-Thiophosphate) and Low Ca Concentration | |||||||||
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![]() | LYASE/LYASE INHIBITOR / ADENYLYL CYCLASE / G(S)ALPHA / ATP-ALPHA-S / CALCIUM ION / LYASE-LYASE INHIBITOR complex | |||||||||
Function / homology | ![]() Adenylate cyclase activating pathway / Hedgehog 'off' state / PKA activation / Adenylate cyclase inhibitory pathway / sensory perception of chemical stimulus / adenylate cyclase / mu-type opioid receptor binding / regulation of insulin secretion involved in cellular response to glucose stimulus / corticotropin-releasing hormone receptor 1 binding / cAMP biosynthetic process ...Adenylate cyclase activating pathway / Hedgehog 'off' state / PKA activation / Adenylate cyclase inhibitory pathway / sensory perception of chemical stimulus / adenylate cyclase / mu-type opioid receptor binding / regulation of insulin secretion involved in cellular response to glucose stimulus / corticotropin-releasing hormone receptor 1 binding / cAMP biosynthetic process / G alpha (z) signalling events / adenylate cyclase activity / beta-2 adrenergic receptor binding / adenylate cyclase binding / D1 dopamine receptor binding / adenylate cyclase-activating adrenergic receptor signaling pathway / cellular response to forskolin / insulin-like growth factor receptor binding / adenylate cyclase activator activity / ionotropic glutamate receptor binding / adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway / cAMP-mediated signaling / G-protein beta/gamma-subunit complex binding / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / adenylate cyclase-activating G protein-coupled receptor signaling pathway / cilium / adenylate cyclase-activating dopamine receptor signaling pathway / heterotrimeric G-protein complex / manganese ion binding / positive regulation of cytosolic calcium ion concentration / intracellular signal transduction / membrane raft / GTPase activity / dendrite / GTP binding / magnesium ion binding / protein-containing complex / ATP binding / membrane / metal ion binding / plasma membrane / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Mou, T.-C. / Sprang, S.R. | |||||||||
![]() | ![]() Title: Structural basis for inhibition of mammalian adenylyl cyclase by calcium. Authors: Mou, T.C. / Masada, N. / Cooper, D.M. / Sprang, S.R. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 161.9 KB | Display | ![]() |
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PDB format | ![]() | 121 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3c14C ![]() 3c15C ![]() 3c16C ![]() 1azsS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Adenylate cyclase type ... , 2 types, 2 molecules AB
#1: Protein | Mass: 25526.514 Da / Num. of mol.: 1 / Fragment: C1A DOMAIN / Mutation: V476M Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 23717.033 Da / Num. of mol.: 1 / Fragment: C2A DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-Protein , 1 types, 1 molecules C
#3: Protein | Mass: 45769.527 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Non-polymers , 7 types, 18 molecules ![](data/chem/img/FKP.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/TAT.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/GSP.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/TAT.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/GSP.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-FKP / |
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#5: Chemical | ChemComp-CA / |
#6: Chemical | ChemComp-TAT / |
#7: Chemical | ChemComp-MG / |
#8: Chemical | ChemComp-GSP / |
#9: Chemical | ChemComp-CL / |
#10: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.97 Å3/Da / Density % sol: 58.53 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion / pH: 5.6 Details: 7.5-7.8% PEG8000, 0.5M NACL, 0.1M PHOSPHATE BUFFER, pH 5.6, VAPOR DIFFUSION, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jul 17, 2008 Details: VERTICAL FOCUSING MIRROR, SINGLE CRYSTAL Si(311) BENT MONOCHROMATOR |
Radiation | Monochromator: Si(311) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0231 Å / Relative weight: 1 |
Reflection | Resolution: 3→25 Å / Num. obs: 20034 / % possible obs: 82.7 % / Redundancy: 2.8 % / Biso Wilson estimate: 31.84 Å2 / Rsym value: 0.175 / Net I/σ(I): 5.1 |
Reflection shell | Resolution: 3→3.077 Å / Redundancy: 2.1 % / Rmerge(I) obs: 0.0305 / Mean I/σ(I) obs: 3.3 / Num. unique all: 1606 / Rsym value: 0.414 / % possible all: 2.95 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 1AZS Resolution: 3→25 Å / Cor.coef. Fo:Fc: 0.861 / Cor.coef. Fo:Fc free: 0.776 / SU B: 25.196 / SU ML: 0.454 / Isotropic thermal model: ISOTROPIC / Cross valid method: THROUGHOUT / ESU R: 1.811 / ESU R Free: 0.563 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 31.84 Å2
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Refinement step | Cycle: LAST / Resolution: 3→25 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3→3.077 Å / Total num. of bins used: 20
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