[English] 日本語
![](img/lk-miru.gif)
- PDB-3ma5: Crystal structure of the tetratricopeptide repeat domain protein ... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 3ma5 | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of the tetratricopeptide repeat domain protein Q2S6C5_SALRD from Salinibacter ruber. Northeast Structural Genomics Consortium Target SrR115c. | ||||||
![]() | Tetratricopeptide repeat domain protein | ||||||
![]() | structural genomics / unknown function / Tetratricopeptide repeat domain protein / NESG / PSI-2 / Protein Structure Initiative / Northeast Structural Genomics Consortium | ||||||
Function / homology | ![]() Tetratricopeptide repeat / Tetratricopeptide repeat domain / Tetratricopeptide repeat / TPR repeat profile. / Tetratricopeptide repeats / Tetratricopeptide repeat / Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat / Alpha Horseshoe / Tetratricopeptide-like helical domain superfamily / Mainly Alpha Similarity search - Domain/homology | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Vorobiev, S. / Neely, H. / Seetharaman, J. / Wang, H. / Foote, E.L. / Ciccosanti, C. / Mao, L. / Xiao, R. / Acton, T.B. / Montelione, G.T. ...Vorobiev, S. / Neely, H. / Seetharaman, J. / Wang, H. / Foote, E.L. / Ciccosanti, C. / Mao, L. / Xiao, R. / Acton, T.B. / Montelione, G.T. / Hunt, J.F. / Tong, L. / Northeast Structural Genomics Consortium (NESG) | ||||||
![]() | ![]() Title: Crystal structure of the tetratricopeptide repeat domain protein Q2S6C5_SALRD from Salinibacter ruber. Authors: Vorobiev, S. / Neely, H. / Seetharaman, J. / Wang, H. / Foote, E.L. / Ciccosanti, C. / Mao, L. / Xiao, R. / Acton, T.B. / Montelione, G.T. / Hunt, J.F. / Tong, L. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 78.1 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 59.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 453.6 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 465.2 KB | Display | |
Data in XML | ![]() | 14.6 KB | Display | |
Data in CIF | ![]() | 19.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2kclS S: Starting model for refinement |
---|---|
Similar structure data | |
Other databases |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
3 | ![]()
| ||||||||
4 | ![]()
| ||||||||
Unit cell |
| ||||||||
Details | Dimer according to gel filtration |
-
Components
#1: Protein | Mass: 11618.613 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.51 Å3/Da / Density % sol: 51.09 % |
---|---|
Crystal grow | Temperature: 291 K / Method: microbatch under paraffin oil / pH: 7 Details: 45-60% PEG 400, 0.03-0.05M potassium chloride, 0.1M Bis-Tris, pH 7.0, microbatch under Paraffin oil, temperature 291K |
-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
---|---|
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Mar 15, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97869 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→50 Å / Num. all: 22394 / Num. obs: 22372 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 21.5 % / Rmerge(I) obs: 0.076 / Net I/σ(I): 59.1 |
Reflection shell | Resolution: 2.8→2.9 Å / Redundancy: 22 % / Rmerge(I) obs: 0.941 / Mean I/σ(I) obs: 3.47 / Num. unique all: 2244 / % possible all: 100 |
-
Processing
Software |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2KCL Resolution: 2.8→44.885 Å / SU ML: 0.42 / Cross valid method: THROUGHOUT / σ(F): 1.25 / Stereochemistry target values: ML Details: There are four molecules (two dimers) in AU. The fourth molecule (D) has very bad defined electron density and was not found by MR. Based on the partial electron density and dimer structure ...Details: There are four molecules (two dimers) in AU. The fourth molecule (D) has very bad defined electron density and was not found by MR. Based on the partial electron density and dimer structure C-terminal part (78-145) of molecule D was built; however B-factor for D molecule is higher than for A-C molecules.
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 72.224 Å2 / ksol: 0.333 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→44.885 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
|