- PDB-3m9i: Electron crystallographic structure of lens Aquaporin-0 (AQP0) (l... -
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Basic information
Entry
Database: PDB / ID: 3m9i
Title
Electron crystallographic structure of lens Aquaporin-0 (AQP0) (lens MIP) in E. coli polar lipids
Components
Lens fiber major intrinsic protein
Keywords
MEMBRANE PROTEIN / water channel / lens / lipid-protein interactions
Function / homology
Function and homology information
gap junction-mediated intercellular transport / water transport / water channel activity / structural constituent of eye lens / gap junction / response to stimulus / lens development in camera-type eye / positive regulation of cell adhesion / visual perception / protein homotetramerization ...gap junction-mediated intercellular transport / water transport / water channel activity / structural constituent of eye lens / gap junction / response to stimulus / lens development in camera-type eye / positive regulation of cell adhesion / visual perception / protein homotetramerization / calmodulin binding / apical plasma membrane / endoplasmic reticulum / plasma membrane Similarity search - Function
Glycerol uptake facilitator protein / Glycerol uptake facilitator protein. / Aquaporin transporter / Major intrinsic protein, conserved site / MIP family signature. / Major intrinsic protein / Major intrinsic protein / Aquaporin-like / Up-down Bundle / Mainly Alpha Similarity search - Domain/homology
Journal: EMBO J / Year: 2010 Title: Principles of membrane protein interactions with annular lipids deduced from aquaporin-0 2D crystals. Authors: Richard K Hite / Zongli Li / Thomas Walz / Abstract: We have previously described the interactions of aquaporin-0 (AQP0) with dimyristoyl phosphatidylcholine (DMPC) lipids. We have now determined the 2.5 A structure of AQP0 in two-dimensional (2D) ...We have previously described the interactions of aquaporin-0 (AQP0) with dimyristoyl phosphatidylcholine (DMPC) lipids. We have now determined the 2.5 A structure of AQP0 in two-dimensional (2D) crystals formed with Escherichia coli polar lipids (EPLs), which differ from DMPC both in headgroups and acyl chains. Comparison of the two structures shows that AQP0 does not adapt to the different length of the acyl chains in EPLs and that the distance between the phosphodiester groups in the two leaflets of the DMPC and EPL bilayers is almost identical. The EPL headgroups interact differently with AQP0 than do those of DMPC, but the acyl chains in the EPL and DMPC bilayers occupy similar positions. The interactions of annular lipids with membrane proteins seem to be driven by the propensity of the acyl chains to fill gaps in the protein surface. Interactions of the lipid headgroups may be responsible for the specific interactions found in tightly bound lipids but seem to have a negligible effect on interactions of generic annular lipids with membrane proteins.
History
Deposition
Mar 22, 2010
Deposition site: RCSB / Processing site: RCSB
Revision 1.0
May 12, 2010
Provider: repository / Type: Initial release
Revision 1.1
Jul 13, 2011
Group: Version format compliance
Revision 1.2
Nov 8, 2017
Group: Data collection / Data processing / Refinement description Category: em_3d_reconstruction / em_image_scans / software
Mass: 18.015 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: ELECTRON CRYSTALLOGRAPHY / Number of used crystals: 281
EM experiment
Aggregation state: 2D ARRAY / 3D reconstruction method: electron crystallography
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Sample preparation
Component
Name: lens Aquaporin 0 / Type: COMPLEX
Buffer solution
pH: 8
Specimen
Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES Details: Purified membranes were solubilized in 4% (w/v) octyl glucoside in 10 mM Tris (pH 8.0) for 30 min at 22C
Crystal grow
Temperature: 300 K / Method: microdialysis / pH: 6 Details: 100 mM NaCl, 50mM MgCl2, 10mM MES pH 6.0, MICRODIALYSIS, temperature 300K
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Data collection
Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company
Microscopy
Model: FEI POLARA 300
Electron gun
Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
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