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- PDB-3ljf: The X-ray structure of iron superoxide dismutase from Pseudoalter... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3ljf | |||||||||
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Title | The X-ray structure of iron superoxide dismutase from Pseudoalteromonas haloplanktis (crystal form II) | |||||||||
![]() | iron superoxide dismutase | |||||||||
![]() | OXIDOREDUCTASE / cold adaptation / superoxide dismutase / flexibility / thermal stability / psychrophilic protein / Metal-binding | |||||||||
Function / homology | ![]() superoxide dismutase / superoxide dismutase activity / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Merlino, A. / Russo Krauss, I. / Rossi, B. / Conte, M. / Vergara, A. / Sica, F. | |||||||||
![]() | ![]() Title: Structure and flexibility in cold-adapted iron superoxide dismutases: the case of the enzyme isolated from Pseudoalteromonas haloplanktis. Authors: Merlino, A. / Russo Krauss, I. / Castellano, I. / De Vendittis, E. / Rossi, B. / Conte, M. / Vergara, A. / Sica, F. #1: Journal: Protein and Peptide letters / Year: 2008 Title: Crystallization and preliminary x-ray diffraction studies of a psychrophilic iron superoxide dismutase from Pseudoalteromonas haloplanktis Authors: Merlino, A. / Russo Krauss, I. / Castellano, I. / De Vendittis, E. / Vergara, A. / Sica, F. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 173.1 KB | Display | ![]() |
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PDB format | ![]() | 136.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 1.8 MB | Display | ![]() |
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Full document | ![]() | 1.8 MB | Display | |
Data in XML | ![]() | 35.8 KB | Display | |
Data in CIF | ![]() | 51.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3lioC ![]() 3lj9C ![]() 1isaS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 21269.508 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: TAC 125 / Gene: sodB, PSHAa1215 / Production host: ![]() ![]() #2: Polysaccharide | alpha-D-glucopyranose-(1-1)-alpha-D-glucopyranose / trehalose #3: Chemical | ChemComp-FE / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.7 Å3/Da / Density % sol: 54.41 % |
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 7 Details: Crystals of protein were obtained using 2.3-2.5M ammonium sulphate, 10 mM FeCl3, 100 mM HEPES pH 7.0, 3% (v/v) isopropanol, VAPOR DIFFUSION, HANGING DROP |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU SATURN 944 / Detector: CCD / Date: Jun 26, 2007 |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→30 Å / Num. all: 51958 / Num. obs: 51958 / % possible obs: 99.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.6 % / Rmerge(I) obs: 0.092 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1ISA Resolution: 2.1→30 Å / Isotropic thermal model: isotropic / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.1→30 Å
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