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- PDB-3lio: X-ray structure of the iron superoxide dismutase from pseudoalter... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3lio | |||||||||
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Title | X-ray structure of the iron superoxide dismutase from pseudoalteromonas haloplanktis (crystal form I) | |||||||||
![]() | iron superoxide dismutase | |||||||||
![]() | OXIDOREDUCTASE / cold adaptation / superoxide dismutase / flexibility / thermal stability / psychrophilic protein / Metal-binding | |||||||||
Function / homology | ![]() superoxide dismutase / superoxide dismutase activity / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Merlino, A. / Russo Krauss, I. / Rossi, B. / Conte, M. / Vergara, A. / Sica, F. | |||||||||
![]() | ![]() Title: Structure and flexibility in cold-adapted iron superoxide dismutases: the case of the enzyme isolated from Pseudoalteromonas haloplanktis. Authors: Merlino, A. / Russo Krauss, I. / Castellano, I. / De Vendittis, E. / Rossi, B. / Conte, M. / Vergara, A. / Sica, F. #1: ![]() Title: Crystallization and preliminary X-ray diffraction studies of a psychrophilic iron superoxide dismutase from Pseudolateromonas haloplanktis Authors: Merlino, A. / Russo Krauss, I. / Castellano, I. / De Vendittis, E. / Vergara, A. / Sica, F. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 99.3 KB | Display | ![]() |
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PDB format | ![]() | 74.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.2 MB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 21.9 KB | Display | |
Data in CIF | ![]() | 32.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3lj9C ![]() 3ljfC ![]() 1isaS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 21269.508 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: TAC 125 / Gene: PSHAa1215, sodB / Production host: ![]() ![]() #2: Polysaccharide | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 52.76 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 1.8M ammonium sulphate, 1.0M sodium chloride, 0.1M Hepes pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Sep 16, 2008 / Details: mirrors |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→30 Å / Num. all: 66818 / Num. obs: 66818 / % possible obs: 99.5 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.06 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1ISA Resolution: 1.5→14.85 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 2 / σ(I): 2 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.5→14.85 Å
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