Pantoate--beta-alanine Ligase; Chain: A,domain 2 - #40 / Pantoate--beta-alanine Ligase; Chain: A,domain 2 / Green Fluorescent Protein / Green fluorescent protein / Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / Beta Barrel / 2-Layer Sandwich ...Pantoate--beta-alanine Ligase; Chain: A,domain 2 - #40 / Pantoate--beta-alanine Ligase; Chain: A,domain 2 / Green Fluorescent Protein / Green fluorescent protein / Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / Beta Barrel / 2-Layer Sandwich / Mainly Beta / Alpha Beta Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 278 / Source method: isolated from a natural source / Formula: H2O
Compound details
IN THIS FLUORESCENT PROTEIN THE CHROMOPHORE MOIETY FORMED FROM RESIDUES LEU66-TYR67-GLY68, (0YG66), ...IN THIS FLUORESCENT PROTEIN THE CHROMOPHORE MOIETY FORMED FROM RESIDUES LEU66-TYR67-GLY68, (0YG66), UNDERGOES HYDROLYTIC DEGRADATION THAT RESULT IN PEPTIDE BOND CLEAVAGE OF THE PROTEIN BETWEEN RESIDUES 66 AND 67 AND ALSO DECARBOXYLATION OF LEU66.HERE, LEU66 IS CLEAVED OUT FROM THE CHROMOPHORE, DECARBOXYLATED BUT REMAINS ATTACHED TO PHE65. DEGRADED CHROMOPHORE MOIETY IS COMPOSED OF RESIDUES TYR67-GLY68 ONLY
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.92 Å3/Da / Density % sol: 57.89 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5 Details: 0.1M Citric Acid, 1.0M LiCl, 20% w/v PEG 6000, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
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