negative regulation of sodium ion transmembrane transporter activity / HECT-type E3 ubiquitin transferase / regulation of dendrite morphogenesis / Degradation of DVL / negative regulation of canonical Wnt signaling pathway / protein polyubiquitination / positive regulation of protein catabolic process / ubiquitin protein ligase activity / ubiquitin-dependent protein catabolic process / proteasome-mediated ubiquitin-dependent protein catabolic process ...negative regulation of sodium ion transmembrane transporter activity / HECT-type E3 ubiquitin transferase / regulation of dendrite morphogenesis / Degradation of DVL / negative regulation of canonical Wnt signaling pathway / protein polyubiquitination / positive regulation of protein catabolic process / ubiquitin protein ligase activity / ubiquitin-dependent protein catabolic process / proteasome-mediated ubiquitin-dependent protein catabolic process / protein ubiquitination / cytoplasm / cytosol 類似検索 - 分子機能
THE COMPLETE SEQUENCE USED FOR CRYSTALLIZATION IS: ...THE COMPLETE SEQUENCE USED FOR CRYSTALLIZATION IS: GSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHAN YSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELP RGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNG. THE PROTEIN WAS CRYSTALLIZED IN THE PRESENCE OF TRYPSIN AND THE ACTUAL CRYSTALLIZED PROTEIN SEQUENCE MAY BE SHORTER. ONLY THE ORDERED PART OF THE PROTEIN IS REPORTED IN SEQRES RECORDS.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 1.75 Å3/Da / 溶媒含有率: 29.83 %
結晶化
温度: 291.1 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 8 詳細: equal volumes of protein (10 mg/ml) [containing 0.1 MM actetic acid PH 3.0] was mixed with crystallization buffer (1.0 M sodium citrate, 0.1 MM immidazole PH 8.0), along with trypsin at a ...詳細: equal volumes of protein (10 mg/ml) [containing 0.1 MM actetic acid PH 3.0] was mixed with crystallization buffer (1.0 M sodium citrate, 0.1 MM immidazole PH 8.0), along with trypsin at a concentration of 1 mg trypsin/500 mg protein. cryoprotected with 15% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 291.1K
解像度: 1.8→1.83 Å / 冗長度: 4.2 % / Mean I/σ(I) obs: 5.462 / Num. unique all: 412 / Rsym value: 0.256 / % possible all: 99.8
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解析
ソフトウェア
名称
バージョン
分類
HKL-3000
データ収集
SOLVE
位相決定
REFMAC
5.5.0102
精密化
HKL-2000
データ削減
HKL-2000
データスケーリング
精密化
構造決定の手法: 単波長異常分散 / 解像度: 1.8→29.45 Å / Cor.coef. Fo:Fc: 0.969 / Cor.coef. Fo:Fc free: 0.943 / SU B: 5.608 / SU ML: 0.081 / 交差検証法: THROUGHOUT / ESU R: 0.139 / ESU R Free: 0.13 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. ATOM RECORD CONTAINS SUM OF TLS AND RESIDUAL B FACTORS. ANISOU RECORD CONTAINS SUM OF TLS AND RESIDUAL U FACTORS
Rfactor
反射数
%反射
Selection details
Rfree
0.19956
413
4.9 %
RANDOM
Rwork
0.14995
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obs
0.15228
7986
99.79 %
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all
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8513
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: MASK