RNA-bindingprotein39 / Coactivator of activating protein 1 and estrogen receptors / Coactivator of AP-1 and ERs / RNA- ...Coactivator of activating protein 1 and estrogen receptors / Coactivator of AP-1 and ERs / RNA-binding motif protein 39 / RNA-binding region-containing protein 2 / Transcription coactivator CAPER
Mass: 12573.253 Da / Num. of mol.: 2 / Fragment: UNP residues 418-530 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: BC030493, Caper, Rbm39, Rnpc2 / Plasmid: SpeedET / Production host: Escherichia Coli (E. coli) / Strain (production host): HK100 / References: UniProt: Q8VH51
Mass: 18.015 Da / Num. of mol.: 184 / Source method: isolated from a natural source / Formula: H2O
Sequence details
SEQUENCE THE CONSTRUCT WAS EXPRESSED WITH AN N-TERMINAL PURIFICATION TAG MGSDKIHHHHHHENLYFQG. THE ...SEQUENCE THE CONSTRUCT WAS EXPRESSED WITH AN N-TERMINAL PURIFICATION TAG MGSDKIHHHHHHENLYFQG. THE TAG WAS REMOVED WITH TEV PROTEASE LEAVING ONLY A GLYCINE (0) FOLLOWED BY RESIDUES 418-530 OF THE TARGET SEQUENCE. NUMBERING IS BASED ON ISOFORM 1 OF UNIPROTKB Q8VH51.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.01 Å3/Da / Density % sol: 38.72 %
Crystal grow
Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 5 Details: 20.00% polyethylene glycol 6000, 0.1M sodium citrate pH 5.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K
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Data collection
Diffraction
Mean temperature: 296 K / Ambient temp details: room temperature Crystal support: loop mounted and sealed with a MiTeGen MicroRT polyester capillary
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