SUMO activating enzyme complex / SUMO activating enzyme activity / protein localization to nuclear pore / negative regulation of transcription by transcription factor localization / SUMOylation of nuclear envelope proteins / ubiquitin activating enzyme activity / Negative regulation of activity of TFAP2 (AP-2) family transcription factors / SUMO is proteolytically processed / negative regulation of delayed rectifier potassium channel activity / SUMO is conjugated to E1 (UBA2:SAE1) ...SUMO activating enzyme complex / SUMO activating enzyme activity / protein localization to nuclear pore / negative regulation of transcription by transcription factor localization / SUMOylation of nuclear envelope proteins / ubiquitin activating enzyme activity / Negative regulation of activity of TFAP2 (AP-2) family transcription factors / SUMO is proteolytically processed / negative regulation of delayed rectifier potassium channel activity / SUMO is conjugated to E1 (UBA2:SAE1) / nuclear stress granule / PML body organization / SUMO is transferred from E1 to E2 (UBE2I, UBC9) / negative regulation of action potential / small protein activating enzyme binding / positive regulation of protein sumoylation / SUMO binding / septin ring / regulation of calcium ion transmembrane transport / SUMOylation of DNA methylation proteins / SUMOylation of immune response proteins / XY body / SUMOylation of SUMOylation proteins / ATP-dependent protein binding / Maturation of nucleoprotein / 転移酵素; アシル基を移すもの; アミノアシル基を移すもの / SUMOylation of RNA binding proteins / regulation of cardiac muscle cell contraction / ubiquitin-like protein conjugating enzyme binding / Postmitotic nuclear pore complex (NPC) reformation / Maturation of nucleoprotein / positive regulation of protein targeting to mitochondrion / negative regulation of protein import into nucleus / roof of mouth development / SUMOylation of ubiquitinylation proteins / ubiquitin-specific protease binding / negative regulation of DNA binding / ubiquitin-like protein ligase binding / SUMOylation of DNA replication proteins / transcription factor binding / SUMOylation of transcription factors / protein sumoylation / potassium channel regulator activity / SUMOylation of DNA damage response and repair proteins / Regulation of IFNG signaling / nuclear pore / enzyme activator activity / cellular response to cadmium ion / SUMOylation of chromatin organization proteins / SUMOylation of transcription cofactors / SUMOylation of intracellular receptors / positive regulation of protein-containing complex assembly / PKR-mediated signaling / negative regulation of DNA-binding transcription factor activity / PML body / Formation of Incision Complex in GG-NER / protein tag activity / regulation of protein localization / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / cellular response to heat / transferase activity / nuclear membrane / nuclear body / protein stabilization / nuclear speck / protein heterodimerization activity / DNA repair / negative regulation of DNA-templated transcription / ubiquitin protein ligase binding / nucleolus / enzyme binding / magnesium ion binding / RNA binding / nucleoplasm / ATP binding / nucleus / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能
THE CYS-GLY-GLY-AVSN LIGAND, WHERE AVSN HAS ID VMX IN THIS FILE, WAS LINKED TO SUMO1 BY INTEIN- ...THE CYS-GLY-GLY-AVSN LIGAND, WHERE AVSN HAS ID VMX IN THIS FILE, WAS LINKED TO SUMO1 BY INTEIN-MEDIATED LIGATION. THERE IS A CROSS-LINK BETWEEN ATOM SG IN RESIDUE CYS 173 B AND C10 IN LIGAND VMX 97 D
配列の詳細
CHAIN D IS COMPOSED AS UB/UBI-CYS-GLY-GLY-AVSN.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 2
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試料調製
結晶
マシュー密度: 2.36 Å3/Da / 溶媒含有率: 47.83 %
結晶化
温度: 279 K / 手法: 蒸気拡散法, ハンギングドロップ法 詳細: 22% PEG 2000 MME, 0.2 M di-ammonium tartrate, 3% EtGly, VAPOR DIFFUSION, HANGING DROP, temperature 279K
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データ収集
回折
ID
平均測定温度 (K)
Crystal-ID
1
100
1
2
1
1,2
1
放射光源
由来
サイト
ビームライン
ID
波長 (Å)
シンクロトロン
APS
24-ID-C
1
0.979
シンクロトロン
APS
24-ID-C
2
0.979
検出器
タイプ
ID
検出器
日付
ADSC QUANTUM 315
1
CCD
2008年10月27日
ADSC QUANTUM 315
2
CCD
2008年11月9日
放射
ID
プロトコル
単色(M)・ラウエ(L)
散乱光タイプ
Wavelength-ID
1
SINGLEWAVELENGTH
M
x-ray
1
2
SINGLEWAVELENGTH
M
x-ray
1
放射波長
波長: 0.979 Å / 相対比: 1
反射
解像度: 2.6→25 Å / Num. obs: 33019 / % possible obs: 99 % / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.074 / Net I/σ(I): 15.8
反射 シェル
解像度: 2.6→2.67 Å / Rmerge(I) obs: 0.367 / Mean I/σ(I) obs: 2.7 / % possible all: 96.1